6
3.9
Connect the incubator to an earthed power supply using the power cord provided. The display
will illuminate immediately. NB:- Do not programme the CO
2
level yet
3.10
The chamber set points are pre-programmed at 37.0°C and 0% CO
2
. Leave the incubator
until the programmed chamber temperature has been reached.
3.11
Carefully unpack the humidity tray. Fill with 0.5 litres of warm distilled water (at approx.
37.0°C). For cell culture work – we recommend the use of copper sulphate (or recognised
biocide) in the humidity tray (one small teaspoonful copper sulphate recommended).
For IVF and other sensitive work – we do not recommend the use of any biocide in the
humidity tray. To reduce the possibility of contamination, the humidity tray water should be
changed and the humidity tray cleaned with 70% Alcohol / 30% distilled water solution every
10 – 14 days. To remove / replace the humidity tray: - turn the tray through 45
°
and remove
for refilling.. To fit the humidity tray - Turn the humidity tray through 45
°
and pull it forward as
shown in the pictures on the following page:-
NB – ensure that the Humidity Tray is located centrally between the racks and pushed fully
back against the stop as shown above right.
3.13
Fit the shelves and leave the incubator for at least two hours (preferably overnight) to allow
conditions to stabilise.
3.14
Programme the CO
2
level (i.e. 5%) and ensure that the CO
2
level stabilises at the
programmed level
3.15
To carry out the Auto Zero, press the
‘
∗
’
and
‘
↵
’
keys simultaneously. The Inner Door must
be opened and closed according to the displayed instructions, after which the process is
completely automatic.
3.16
The incubator is now ready for use.
