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RotaChrom rCPC Operation Manual
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New seals should be run in before first use.
Disconnect all equipment connected to the pump output.
Connect reduction valve (or another reliable hydrodynamic resistor) directly to the pump output. Put
the inlet tubing and output capillary into same reservoir with solvent isopropanol: water 1:1. Set the
flow rate to 300 ml/min., start pumping and set pressure at 2MPa using reduction valve. Let the
pumping for at least 30 min (optimally 2 h). Keep the flow rate at 300 ml/min. and set pressure at 5
MPa. Let the pumping for at least 30 min (optimally 2h). Keep the flow rate at 300 ml/min. and set
pressure at 7 MPa. Let the pumping for at least 10 min (optimally 30 min.). Then set flow rate at 30
ml/min. and pressure at 2 MPa. Let the pumping for at least 10 min (optimally 30 min). Seals are now
Remove the seal using a suitable
tool. Wash and clean the piston
and interior of the head with
isopropanol.
Usually it is not necessary to
exchange the seal in washing
heads, but in case it is needed,
follow the same steps as with
working heads.
Wet new seal in isopropanol and
place it on the piston carefully.
The spring is facing toward the
head.
Pushing the head back on the
piston must be straight.
Leaning
the head to sides may cause
seal damage.
Fasten the screws and mount
tubing and capillary back on their
place. The steps for mounting are
same as for dismounting, but in
reverse order.
