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RotaChrom rCPC Operation Manual

Revision History

This document applies to the latest software listed and higher versions. When a subsequent software
version changes the information in this document, a new issue will be released.

OPM-rCPC-121818, December 2018

Software version 1.0

This document was created to:

■

Replace the previous Operation Manual

■

Improve the content and usability of the instructions

■

Update the Hazards section

Summary of Contents for rCPC

Page 1: ...RotaChrom rCPC Operation Manual Version number OPM rCPC 121818 Reviewed by Attila Babits Issued by Zsuzsanna Friedman RotaChrom Technologies LLC 151 F t Dabas 2370 Hungary...

Page 2: ...ts Reserved by RotaChrom Technology LLC Operation Manual rCPC OPM rCPC 121818 2018 RotaChrom Technologies LLC All Rights Reserved All other trademarks are the property of their respective owners Find...

Page 3: ...ftware listed and higher versions When a subsequent software version changes the information in this document a new issue will be released OPM rCPC 121818 December 2018 Software version 1 0 This docum...

Page 4: ...ed in your contractual agreement for the system The customer is responsible for routine preventive maintenance procedures The cost of repairs arising from the failure to perform these maintenance proc...

Page 5: ...ystem The hazards of individual procedures in this manual are included in the warnings or cautions within the instructions Read this section before you operate this system Follow the power requirement...

Page 6: ...gy can cause interference with other equipment In addition other equipment can radiate radio frequency energy to which the system is sensitive If you suspect interference between the system and other...

Page 7: ...eference and one copy offsite Dedicate the computer hardware to only running the system software Do not connect the computer hardware to the Internet unless instructed to do so by RotaChrom Keep the s...

Page 8: ...d creating in the software 20 Cleaning and maintenance 23 Cleaning 23 Cell disassembly for cleaning and cleaning validation 23 Cell assembly after cleaning 24 Maintenance and service 28 Mounting of th...

Page 9: ...es are liquids and the liquid stationary phase is immobilized by a strong centrifugal force CPC system consists of two interconnected parts 1 Preparative unit composed of the following items Stainless...

Page 10: ...mains outlet Before removing any cover or removing safety screws of the front door shut down the system and remove mains plug RotaChrom cell design RotaChrom s unique cell design represents the Keep I...

Page 11: ...onnect some connections and reinstall them by the instructions described in this chapter The flow connection is described on the figure below 5 figure schematic of flow connection 6 figure operation o...

Page 12: ...pump with 3 16 FEP tubing 7 figure Gradient box 8 figure Pump These FEP tubing utilize PEEK nuts with inverted ferrules Pump should be connected to the ASC DSC valve with 1 8 SS tubing with VICI nut a...

Page 13: ...nd and VICI nut and SS PEEK ferrule at valve Valve should be connected to the detector with inverted ferrule at detector end 10 figure UV VIS detector Detector should be connected to fraction collecto...

Page 14: ...the front and rear of the rotor connection The rotary seals are standard units see spare parts list and are connected to rotor with G threads and can be removed by a wrench There is a Swagelok 1 8 tu...

Page 15: ...of peripherals Default IP setting for the Instrument Data server is DHCP assigned It is recommended to note the MAC address of the instrument and set a fixed IP assignment on router contact IT based o...

Page 16: ...tact info rotachrom com or 36 70 885 6922 for registration key Normally the registration is done by RotaChrom during the installation After you execute ECOMAC application you can see next window or si...

Page 17: ...Method configuration dialog by choosing Method Devices On the left side of section are displayed supported devices to you 18 figure Device configuration window You can add selected device to the right...

Page 18: ...e s identification if available Most of the parameters are disabled often because other settings are not possible or parameter is not used 20 figure Communication test of device After communication te...

Page 19: ...s etc and you can observe this process at real time You can change devices parameters now through Devices On line Configuration dialog click on Method Online configuration or press F10 If ready state...

Page 20: ...velength The end of the start method the set up speed of the rotor has to be the operating speed 24 figure Start method writing Secondly the operator has to write a loop method In the loop method inje...

Page 21: ...as to write a stop method It includes stopping the pump and the rotor speed That means the separation is finished 26 figure Loop method writing Fourthly the operator has to write a sequence Here inser...

Page 22: ...ity component 28 figure Chromatogram During the filling with stationary phase it is recommended to pump at least 2 l of new fresh stationary phase to completely exchange previous one especially if dif...

Page 23: ...d with usual detergents 50 ethanol solution and can be wiped with soft cloth During the separation must use filtration For example 20 m filter at the end of the inlet tubing After a purification proce...

Page 24: ...olvent to make a reference solution which is then measured by a sufficient analytical technique for remaining of material under study When cell disassembly is carried out only for swab probing we reco...

Page 25: ...and Fix with torque of 19 20 Nm Connect the cells in line with C type 7 interconnect tubing Connect the cells between lines with L type 8 interconnect tubing When connection the tubes first put on the...

Page 26: ...30 figure schematic of flow connection Cell blocks should be screwed into the rotor on a stand Interconnects between cell blocks should be made with S type interconnects 10 Input and Output interconne...

Page 28: ...ecessary to send the maintenance report Inspection Interval Note Check for leaks At startup purging during sequence Check tubes and nuts for tightness Rotary seals Every week Seals should not leak Rot...

Page 29: ...otor remove any rotary seal tubing and the hex adapter from the axis and make sure that the axis wedge is in place The axis end is slightly conical to ease the putting on of the rotor With the rotor h...

Page 30: ...Reserved by RotaChrom Technology LLC 35 figure inlet and outlet connections on the front and back side of instrument output tube 10 19 hex adapter 11 rotary seal 13 screw 35 33 12 tube holder 32 nut 2...

Page 31: ...procedures disconnect unit from power source by disconnecting the main supply cable The unit may be reconnected to power source only after all service operations are finished Pump piston is made of fr...

Page 32: ...2h Keep the flow rate at 300 ml min and set pressure at 7 MPa Let the pumping for at least 10 min optimally 30 min Then set flow rate at 30 ml min and pressure at 2 MPa Let the pumping for at least 10...

Page 33: ...in supply cable The unit may be reconnected to the power source only after the cover is mounted back on its place UV light is harmful for eyes During regular operation of the detector is UV light perf...

Page 34: ...power supply cord into the instrument socket on the rear panel Unplug serial line cable into connector RS232 for interconnection with PC Connector can be fixed by two screws using cross tip screwdriv...

Page 35: ...t with the new one Connect the tubing with the female nut the new one 37 figure Disconnect of the rotary union 38 figure Disconnected rotary union holder Connect the tubing with the female nut the new...

Page 36: ...flangeless when deformed RA00601 Pump Ceramic piston Leak low flow accuracy RB00101 Working head Teflon piston seal 3 8 Leak low flow accuracy RB00201 Washing head Teflon piston seal 3 8 Leak low flow...

Page 37: ...rmed ferrule Loosen nut exchange ferrule cut from the tubing if necessary and tighten nut Low flow or pressure Piston wear Exchange piston Seal wear Exchange piston seal High pulsation Solvent bubbles...

Page 38: ...ion of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must b...

Page 39: ...especially when toxic or hazardous solvents are used Do not touch the power button and the electrical parts with wet hands During the operation the door of the instrument must be closed In case of em...

Page 40: ...C Operation Manual All Rights Reserved by RotaChrom Technology LLC Secondly switch off the rotation and after the pump on the on line configuration side 42 figure Switch off the rotation 43 figure Swi...

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