41 400-12355-000 Rev. 03
KR2
i
/ KMP
i
TFF Systems
System Functionality
8.2.2
Concentration/Diafiltration Mode
C/D Mode may be used for a Fed-Batch concentration, followed by a Diafiltration.
NOTE:
This constant feed concentration followed by a diafiltration can be done with the CDDC mode
when 2 auxiliary pumps are available (see Section 8.2.4 below). Also the CD mode can be
done without the vacuum addition if the full sample fits in the process vessel on the scale.
On the Feed Reservoir with four ports, set-up the ports as described below to create a vacuum that
will draw excess feed into the Feed Reservoir from the Extra Feed Reservoir:
1.
Feed Line to Filter: Connect tubing from this line through pump head to filter's feed port
2.
Retentate Line from Filter: Connect tubing from filter retentate port to this line
3.
Buffer: Connect through Auxiliary Pump 1 to Diafiltration Buffer Reservoir
4.
Vent: Connect to Extra Feed Reservoir
Feed Reservoir
Module
Permeate Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
Feed Scale
KMP
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Pump
Permeate Pump
(Auxiliary Pump 2)
Permeate Scale
Extra Feed Reservoir
Diafiltration Buffer Reservoir
