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REPLIGEN KrosFlo KPS, User Manual

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REPLIGEN KrosFlo KPS User Manual Download Page 25

 

KrosFlo® Systems 

User Guide 

 

 

 

 

25          repligen.com           IF.UG.028   R1 

 

 

Table 10. Recommended spare parts 

 

12.1

 

Accessing the Scale Settings screen 

The Scale Settings screen is a “hidden” screen that enables you to configure the maximum weight 

value for the scale (

WE-01

) connected to the KrosFlo® System. 

 

To access the Scale Settings screen and configure the maximum weight value for the  

scale (

WE-01

): 

1.

 

Tap the lower right corner on the Alarm Setup screen. 

 

 

 

 

 

Item 

Description 

Quantity 

1 

2 

3 

4 

5 

6 

7 

8 

9 

10 

11 

12 

13 

14 

15 

16 

FU-1, Main power 220VAC 

FU-2, Spare 

FU-2A, Pump P-03 

FU-2B, Spare 

FU-3, PS-2 Input supply 220VAC 

FU-4, PLC Input supply 220VAC 

FU-5, PS-1 Input supply 220VAC 

FU-6, PS-3 Input supply 220VAC 

FU-13, BPCV-01 

FU-17, PS-1 Output 24VDC 

Repligen Fittings Kit 

1 ½” Tri-clamp 

1 ½” Tri-clamp gasket 

1” Tri-clamp gasket 

Permeate tubing 

Retentate tubing 

AGC-10   10A 

 

AGC-5   5A 

 

AGC-5   5A 

AGC-2   2A 

AGC-5   5A 

AGC-10 10A 

AGC-1   1A 

AGC-5   5A 

1 

2 

5 

5 

5 m 

5 m 

Summary of Contents for KrosFlo KPS

Page 1: ...UG 028 R1 KrosFlo Systems User Guide For use with KrosFlo KTF Tangential Flow Filtration TFF System KrosFlo KPS Tangential Flow Filtration TFF System KrosFlo TFDF Tangential Flow Depth Filtration TFDF System ...

Page 2: ... or consequential damages in connection with the furnishing performance or use of this material No part of this document may be photocopied reproduced or translated to another language without the prior written consent of Repligen Corporation Products are not intended for diagnostic or therapeutic use or for use in vivo with humans or animals For further information please contact Repligen Corpora...

Page 3: ...User Guide User Guide KrosFlo Systems 3 IF UG 028 R1 Contents 7 8 1 Warning alarms 20 ...

Page 4: ...s 21 Table 8 System fuses 24 Table 9 Installed major components and identification tags 24 Table 10 Recommended spare parts 25 List of figures Figure 1 Caution and warning examples 8 Figure 2 Polysulfone pressure sensors example 11 Figure 3 Installed flow path pressure sensor ferrite bead 12 Figure 4 Main screen example 14 Figure 5 Settings screen Fed Batch disabled 16 Figure 6 Tubing Selection po...

Page 5: ...terface Kg Kilograms TFDF Tangential Depth Filtration System Lbs Pounds LCD Liquid crystal display lpm Liters per minute M Meter mA Milliampere or milliamp MBT Module bag tubing NWP Normalized Water Permeability PLC Programmable logic controller Psi Pounds per square inch rpm Revolutions per minute SAS Standard alert symbol TFF Tangential Flow Filtration TMP Transmembrane pressure VAC Volts AC pow...

Page 6: ...distinguish the severity level of a hazard Signal word definitions and colors are listed in Table 1 Table 1 Signal words definitions and colors 2 2 Safety symbol icons The typical safety symbol icons hazard prohibition and mandatory action used on Repligen systems are listed in Table 2 The icons are pictograms that communicate hazards quickly and across language barriers Description Safety Alert S...

Page 7: ...epligen com IF UG 028 R1 Table 2 Safety symbol icons Electrical Hazard Heavy Object Heat Hazard Radiation Hazard Crush Hazard Pinch Hazard Inhalation Hazard Authorized and Qualified Personnel Only Read the Manual Noise Level Hazard ...

Page 8: ... plug in the system during Mechanical Assembly WARNING Do not plug the system in during Electrical Connections procedure until instructed WARNING System must be turned off when replacing fuses to prevent electrical hazard Any electrical work beyond the electrical connections procedure and fuse replacement should be performed by trained personnel only WARNING Peristaltic pumps must be turned off wh...

Page 9: ...en Bradley Integrated Panel Display Computer Human Machine Interface ProConnex Flow Path Designed flexible tube flow path from Repligen Support structure Stainless steel filter stand and lab cart from Repligen Weight 300 lb approximately 135 Kg 500 lb approximately 225 Kg Dimensions L x W x H 32 x 24 x 52 5 inches 81 x 61 x 133 cm 47 x 24 x 80 inches 120 x 61 x 203 cm with extension Power requirem...

Page 10: ...ale interface cable If the tanks are more than one accordingly connect the scales as WE 02 WE 03 etc 7 Route and connect all cables Cables are marked based on their destination All cables should be routed to minimize kinking and obstructions 8 Once the filter stand is assembled and pump motor is mounted ready the pump head and flow path MBT assembly items Make sure all necessary components to comp...

Page 11: ...ct P 01 P 02 and P 03 connectors to panel 2 Connect flow meter FL 01 FL 02 connectors to the flow meters 3 Connect three pressure sensor cables PE 01 feed PE 02 retentate PE 03 permeate to the flow patch pressure sensors 4 Connect Main Panel power cord to suitable electrical outlet 200 240VAC 10 15 Amp Service 5 Make sure that the Emergency Stop E Stop button is pulled to OUT inactive position 6 T...

Page 12: ...supplied flow path pressure sensor ferrite bead 1 Locate the ferrite bead as close as possible to the flow path pressure sensor while ensuring there is enough cable to wrap around the ferrite bead 2 Open the ferrite bead 3 Seat the flow path pressure sensor cable in the bead 4 Wrap the cable around the bead 5 Snap the bead closed ...

Page 13: ...n completed To run KrosFlo Systems 1 Pull the E Stop button located on front of Control Panel to the OUT position 2 Turn the front panel power switch to ON position 3 Press the BLUE reset button on control panel Once the computer is booted up use the touchscreen to navigate to the TFF icon on the screen Double click on the TFF icon to enter the KrosFlo Operating System The control system will open...

Page 14: ...point input data is displayed in the BLUE highlighted boxes Auto and Manual modes of operation for the Recirculating and Product Pumps are selected here The system can operate independently outside of an automated process sequence allowing the user greater flexibility in performing trials to optimize filter performance prior to entering a run Setpoint data is entered by touching a BLUE input box T...

Page 15: ...mary recirculation vessel being used The calculations for process estimate the remaining volume based on the permeate flow and scale reading for the main vessel When the main vessel weight reduces below the Level Setpoint During Concentration setpoint for the time defined by the Sample Feed Pump Stop Delay setpoint the KrosFlo System will assume the feed vessel is empty and switch its volume calcu...

Page 16: ... the Filter Model Selecting a filter model will populate the Fiber Count Lumen ID and Surface Area These parameters are used for the calculation of shear and flux In the event a filter is not listed the Fiber Count and Lumen ID must be entered in the BLUE boxes shown in the Filter Model box to ensure correct shear rate data Figure 5 Settings screen Fed Batch disabled The Settings screen also allow...

Page 17: ...k at the start of the concentration process This setting will be used to calculate the Concentration Factor over time based on the remaining volume after the start of the process System Holdup Volume used to compensate for the volume of liquid that is in the flow path during the concentration process Level Setpoint During Concentration the level that the system will maintain in the feed vessel whi...

Page 18: ... 0 100 0 600 0 600 0 100 0 600 0 100 0 300 0 100 0 30 0 85 0 100 Up to 20 liters liters liters kg X kg X seconds DV psi lpm C characters Parameter Range Units Filter Model Filter Model Number Fiber Count Lumen ID MWCO Chemistry Effective Length Surface Area Entry Qty of Modules Up to 13 0 50 000 0 100 Up to 10 Up to 10 Up to 5 Up to 200 000 Up to 1 characters fibers lumen characters characters cha...

Page 19: ... setting the shutdown alarm Table 6 Alarm Setup screen setpoint range Alarm setpoints Range Units Low Feed Pressure PE 01 High Feed Pressure PE 01 Feed High Pressure Shutdown PE 01 Feed High Pressure Duration Low Retentate Pressure PE 02 High Retentate Pressure PE 02 Low Permeate Pressure PE 03 High Permeate Pressure PE 03 High Permeate Volume FL 02 Low Permeate Flow FL 02 High Permeate Flow FL 02...

Page 20: ...he flashing ALARM button once the system is operating in the normal range Alarms can be enabled or disabled manually using the ALARM SETUP screen To disable or prevent an alarm select the Disabled button Disabled should be displayed for alarm to be disabled Likewise Enabled will be displayed if the alarm is enabled High alarms will trigger when process value rises to SP or above Low alarms will tr...

Page 21: ...Table 7 7 9 Chart screen Figure 9 Chart screen example Table 7 Chart screen parameters Data can be viewed on the chart in real time or reviewed from a previous time period Some features of the chart may be customized using the chart menu The chart menu can be accessed by placing cursor on the chart and using left double click Tag Range Units FL 01 FL 02 PE 01 PE 02 PE 03 P 01 TMP Shear Rate Feed F...

Page 22: ...s Screen After input of all Setup data navigate to the Main Screen to initiate a Process run The preferred start up technique is to start the TFF1 process with Pump P 01 in Manual Mode at setpoints below parameters of the selected Auto Mode Once started run the system in Manual Mode for a few minutes to completely flood the MBT and ensure air has been displaced Once this is complete stop the syste...

Page 23: ...nowledge and Reset the Production Stop Alarm The desired sequence will have to be started Restarted by the operator 11 Maintenance The robust design of KrosFlo Systems are intended for use with process equipment As such lab or plant cleanliness and care are all that is needed to ensure quality operation when used in the systems intended manner The frame cabinet and pumps can be cleaned by manual w...

Page 24: ...ing model System P 01 P 02 P 03 FL 01 FL 02 PE 01 02 03 Recirculating Pump Motor Recirculating Pump Head Product Pump Motor Product Pump Head Product Pump Motor Product Pump Head Retentate flowmeter Permeate flowmeter Flow converter Pressure sensors Levitronix LPM 600 3 10 Levitronix DCP 600 MasterFlex 77420 10 I P peristaltic pump MasterFlex MasterFlex 77420 10 I P peristaltic pump MasterFlex Son...

Page 25: ...e for the scale WE 01 1 Tap the lower right corner on the Alarm Setup screen Item Description Quantity 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 FU 1 Main power 220VAC FU 2 Spare FU 2A Pump P 03 FU 2B Spare FU 3 PS 2 Input supply 220VAC FU 4 PLC Input supply 220VAC FU 5 PS 1 Input supply 220VAC FU 6 PS 3 Input supply 220VAC FU 13 BPCV 01 FU 17 PS 1 Output 24VDC Repligen Fittings Kit 1 Tri clamp 1 Tri...

Page 26: ...f transmembrane pressure typical 5 15 PSIG for UF membranes or 2 5 PSIG for MF membranes for a given membrane area were the flow is normalized based upon temperature and is expressed as LMH PSIG 20C This information is used to determine productivity impact to productivity as well as effectiveness of cleaning solution if membrane is going to be re used NWP Post Cleaning Membrane Recovery is defined...

Page 27: ...com IF UG 028 R1 13 Index Alarm 13 19 20 22 23 25 Caution 6 8 Components 6 9 10 22 23 24 Instructions for use 10 Maintenance 23 Note 13 18 22 Safety 6 7 8 23 Setup 13 19 22 25 System overview 13 System specifications 9 Warning 8 10 11 13 23 ...

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