KrosFlo® Systems
User Guide
17 repligen.com IF.UG.028 R1
Press the
0 - 20 lpm/0 -8 lpm
toggle button to specify the range of the Permeate flow meter.
Press
Tubing Selection
to select the size of the tubing to be used with the KTF/KPS/TFDF® peristaltic
pump (Product Pump (P-02)). You may also enter a calibration factor for the selected tubing on this
popup screen.
Figure 6. Tubing Selection popup screen
Press the Fed Batch Enabled / Fed Batch Disabled toggle button to enable or disable fed batch
processing.
With Fed Batch disabled, you may press the Starting Vol = Enter Above/Starting Vol. = Use Scale
toggle button to specify whether the Starting Volume (that is, the volume in the feed tank at the
start of the concentration process) is entered as a setpoint or is based on the weight of the tank
(WE-01).
With Fed Batch enabled, the
Level Setpoint During Concentration
and
Sample Feed Pump Stop
Delay
setpoints are available for configuration.
The process setpoints that may be configured from the Settings screen are defined as follows:
•
Starting Volume
- the volume in the feed tank at the start of the concentration process. This
setting will be used to calculate the Concentration Factor, over time based on the remaining
volume after the start of the process.
•
System Holdup Volume
- used to compensate for the volume of liquid that is in the flow
path during the concentration process.
•
Level Setpoint During Concentration
- the level that the system will maintain in the feed
vessel while in fed batch concentration mode.
•
Initial Concentration Setpoint
- setpoint for the start of the Initial Concentration Sequence,
if run.
•
Final Concentration Setpoint
- setpoint for the end of the Final Concentration Sequence.
•
Sample Feed Pump Stop Delay
- the amount of time that the sample pump will run after the
weight on the feed tank drops below the setpoint volume.
•
DV Setpoint
- end point of the diafiltration sequence. This number signifies the number of
diafiltration volumes that will be processed.
•
TMP Setpoint
- Constant Transmembrane Pressure setting for TFF processes.
•
Recirc Flow Setpoint
- controls the speed of the recirculation pump (P-01).
•
Feed Temp
- used in calculating Normalized Water Permeability (NWP). A description of
lists the ranges of values for all input parameters on the Settings screen.
