Qiagen QIAgility, User Manual

Page: 245 / 334

Instrument Setup and Calibration
QIAgility User Manual 11/2011 7-11
6. Use the up/down arrow buttons in the manual height
calibration window to raise and lower the pipetting
head.
7. To adjust the height correctly, the distance between the
tip and the tube base must be adjusted as described
above. In most cases it is not possible to see the distance
between the tip and the base, the distance must be ‘felt’
by manually lifting the tube from its support plate.
8. If the tip makes forceful contact with the base of the
tube/well, the “Level at bottom of well” does not reflect
the true distance of the base of the tip from the home
position. Use the “Home Z-axis” button to reset the “0”
position and repeat steps 5–7, starting with a lower
“Level at bottom of well” value to avoid driving the tip
forcefully into the base of the well.
9. Click on the “OK” button when the correct height
calibration has been set.
10. Click on the “Cancel” button when all calibrations are
complete.
7.1.4 Optimal height calibration
For optimal height calibration, please refer to “Optimal plate
height calibration” in Appendix F.
7.1.5 Calibrate tip offsets
It is recommended that this calibration is performed upon
initial installation and when the user starts a new lot number
of tips.
The collar size of 50 µl and 200 µl tips should be equal, but
small variations sometimes exist.