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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM248 · Revised 12/18
www.promega.com
4.B. Thermal Cycling: Protocols for use with the Perkin-Elmer Model 480 and GeneAmp
®
PCR System
9600 or 9700 Thermal Cyclers
Place the tubes in the thermal cycler, and run the recommended program. The preferred protocols for use with the
Perkin-Elmer Model 480 and GeneAmp
®
PCR System 9600 and 9700 thermal cyclers are provided below. It may be
necessary to optimize the program with other thermal cyclers.
Note:
It is important to preheat the instrument to 94°C before placing tubes into the machine.
Program for the Perkin-Elmer Model 480 Thermal Cycler
Preheat the thermal cycler to 94°C before placing tubes inside.
Cycling Profile:
94°C for 2 minutes, then:
94° for 1 minute
57°C for 30 seconds
72°C for 1 minute
For
35 cycles
, then:
72°C for 5 minutes
4°C soak
After completion of the thermal cycling protocol, store the samples at –20°C.
Program for the Perkin-Elmer GeneAmp
®
PCR System 9600 Thermal Cycler
Preheat the thermal cycler to 94°C before placing tubes inside.
Cycling Profile:
94°C for 2 minutes, then:
94°C for 30 seconds
Ramp 68 seconds to 58°C, hold 30 seconds
Ramp 50 seconds to 70°C, hold 45 seconds
For
35 cycles
, then:
68°C for 2 minutes
4°C soak
After completion of thermal cycling protocol, store samples at –20°C.
!
