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TM248 · Revised 12/18
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Y Chromosome Deletion
Detection System, Version 2.0
1. Description .........................................................................................................................................2
2. Product Components and Storage Conditions ........................................................................................3
3. System Requirements ..........................................................................................................................3
3.A. Template ....................................................................................................................................3
3.B. Thermal Cyclers .........................................................................................................................4
3.C. Contamination Control ...............................................................................................................4
3.D. Control Reactions .......................................................................................................................5
4. Amplification Reactions .......................................................................................................................6
4.A. Reaction Setup for Large Sample Numbers ...................................................................................6
4.B. Thermal Cycling: Protocols for use with the Perkin-Elmer Model 480 and GeneAmp
®
PCR System
9600 or 9700 Thermal Cyclers .....................................................................................................8
4.C. Agarose Gel Electrophoresis ........................................................................................................9
4.D. Data Analysis—Controls ............................................................................................................ 10
4.E. Data Analysis—Experimental Samples ........................................................................................ 10
5. Troubleshooting................................................................................................................................ 12
6. References ........................................................................................................................................ 14
7. Appendix .......................................................................................................................................... 15
7.A. Composition of Buffers and Solutions ......................................................................................... 15
7.B. Related Products ...................................................................................................................... 15
7.C. Reaction Setup for Small Sample Numbers ................................................................................. 15
7.D. Preparation of Agarose Gels ...................................................................................................... 17
7.E. Worksheets .............................................................................................................................. 18
8. Summary of Changes ......................................................................................................................... 21
