Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
11
www.promega.com
TM248 · Revised 12/18
If multiple amplification bands are missing, and those bands do not map to adjacent regions of the Y chromosome
(Table 2), they represent dropout bands and are not deletions. See the troubleshooting guide (Section 5) for help if you
observe dropout of alleles in individual master mixes.
We have observed some nonspecific bands that appear above or below the expected amplification products on the
agarose gel. If your negative controls show no detectable PCR products, these nonspecific bands should have no effect
on analysis.
Figure 4. Y chromosome deletion analysis.
The amplification products from Multiplex A Master Mix, Multiplex B
Master Mix, Multiplex C Master Mix, Multiplex D Master Mix and Multiplex E Master Mix reactions are shown. Lanes:
1, Male Genomic DNA control; 2, sample male DNA (containing deletions). Bands generated with Male Genomic DNA
control are compared to those generated with a sample containing Y chromosome deletions. (Note the deleted bands in
Multiplex A–D. Multiplex E shows no deletions.) The marker (M) lanes contain the 50bp DNA Step Ladder
(Cat.# G4521).
M
1
2
M
1
2
M
1
2
4389TA11_3A
A.
B.
C.
M
1
2
M
1
2
D.
E.
