6
Prior
Scientific
7. Setting
K
ö
hler Illumination
A.
Follow the procedure in section 6 to set up the instrument for your eyesight.
B.
With a specimen in sharp focus and the analyser in the “out” position using the 10x
objective, close the field diaphragm until it impinges on the field of view, Figure 1,
Picture A.
Figure 1
C.
Focus the condenser using the side mounted rack and pinion controls until the leaves
of the diaphragm are in sharp focus, Figure 1, Picture B.
D.
Using the condenser centration controls move the diaphragm into the centre of the
field of view, it may help to open it until it is nearly touching the outside of the field
to attain perfect centration, Figure 1, Pictures C & D.
E.
Fully open the field diaphragm.
F.
Fully open the condenser diaphragm and then slowly close it until you see the
contrast within the image increase. If you then remove an eyepiece and look directly
down the tube from a distance of 20-30cm you should see an image similar to
picture D in Figure 1. The aim here is to have the “bright” area occupying
approximately 70% of the total field. The amount it occupies will change dependant
on the objective lens in use. If you have set up the instrument using the 10x
objective (recommended) then as you increase the magnification this diaphragm will
need opening to optimise the contrast and resolution. Often it is set for the
objective which is either most frequently used or most critical in terms of resolution
and left in that position
Summary of Contents for Lux POL
Page 1: ...Operating Instructions PriorLux POL Advanced Polarising Microscope...
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Page 6: ...4 Prior Scientific 4 Component Parts...
Page 13: ...PriorLux POL 11...
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