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Photon Systems Instruments AquaPen-C AP 110-C, Instruction Manual

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Photon Systems Instruments AquaPen-C AP 110-C Instruction Manual Download Page 5

 

 

Page | 5 

 

 

1

 

I

NFORMATION BEFORE USING 

A

QUA

P

EN DEVICE

 

 

Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for 
clarification. 

 

By accepting the device, the customer agrees to follow the instructions in this guide. 

 

Always follow corresponding manuals while working with the AquaPen device or doing the maintenance. 

It  is  forbidden  to  interfere  with  the  hardware  or  software  of  the  AquaPen  device  in  any  way  without  previous  agreement  with  the 
manufacturer. 

 

The following table presents basic highlight symbols used in this manual: 

Symbol 

Description 

 

Important information, read carefully. 

 

Complementary and additional information. 

Tab. 1 Used symbols. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Summary of Contents for AquaPen-C AP 110-C

Page 1: ......

Page 2: ...he specifications of the device However deviations cannot be ruled out Therefore a complete correspondence between the manual and the real device cannot be guaranteed The information in this manual is...

Page 3: ...rement 18 5 1 3 OJIP protocol 18 5 1 4 Non photochemical quenching NPQ protocols 20 5 1 5 Light curve LC protocols 23 5 2 Optical density measurement AquaPen C only 26 5 2 1 Calibration 26 5 2 2 Measu...

Page 4: ...Page 4 10 1 GPS AquaPen operation 50 10 2 Data download 50 11 Warranty terms and conditions 52 12 Troubleshooting and Customer Support 52...

Page 5: ...instructions in this guide Always follow corresponding manuals while working with the AquaPen device or doing the maintenance It is forbidden to interfere with the hardware or software of the AquaPen...

Page 6: ...teria which tend to absorb inefficiently the blue light AquaPen P AP 110 P is a probe version which allows detection of chlorophyll fluorescence in liquid samples by directly submersing the probe in t...

Page 7: ...logging Up to 149 000 measurements depending on protocol Data transfer USB cable Bluetooth transfer up to 3Mbps for distance up to 20m PC software FluorPen 1 1 Windows 7 and higher Battery Li Ion rec...

Page 8: ...C ID T9J RN42 Europe ETSI EN 301 489 1 V1 8 1 ETSI EN 301 489 17 V2 1 1 ETSI EN 300 328 V1 7 1 Canada IC RSS 210 low power comm device Certification number 6514A RN42 EMC USA FCC CFR47 Part 15 subclas...

Page 9: ...Page 9 3 DEVICE DESCRIPTION Fig 1 Device description...

Page 10: ...CE AquaPen P AP 110 P Never submerge the whole device in the liquid Only the optical tip can be submerged Rinse the optical tip of the AquaPen P in freshwater after each use Inspect visually the optic...

Page 11: ...measures optical density at 680 and 720 nm To use measurements of chlorophyll fluorescence to analyze photosynthesis researchers must distinguish between photochemical quenching and non photochemical...

Page 12: ...performed with a dark adapted sample see More in chapter 5 1 4 LC Light Curve Photosystem II Quantum Yield estimated from fluorescence that is measured sequentially at several different light levels...

Page 13: ...Page 13 Fig 3 AquaPen C AP110 C Fig 4 AquaPen P AP 110 P...

Page 14: ...es intensity The measuring pulses are weak light pulses which are able to induce the minimal chlorophyll fluorescence F0 or Ft It takes only 30 s and the maximum intensity is 3 000 mol m 2 s 1 It mean...

Page 15: ...pted suspension following the first exposure to flash pulse during QY measurement the sample may be inhibited and it is recommended to use a new dark adapted sample for future measurements or allow su...

Page 16: ...easurements of OJIP or sufficient time should be allowed for the sample to be dark adapted again The Super pulse setting recommended by manufacturer is 80 When performing the OJIP measurement with dif...

Page 17: ...Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application If Overflow is observed on display during measurement there are two options to r...

Page 18: ...s to be download from the AquaPen to the PC computer via USB cable or the Bluetooth connection using FluorPen Software page 44 All measured data are stored in the device memory and can be downloaded t...

Page 19: ...curve between F40 s and F1s background subtracted SM SM Area Fm F0 multiple turn over SS SS the smallest SM turn over single turn over N N SM M0 1 VJ turn over number QA Phi_P0 Phi_P0 1 F0 Fm or Fv F...

Page 20: ...n pulses See Table 1 NPQ Protocols Table 1 Phase Duration of pulses 1st pulse Pulse interval NPQ1 Light 60 s 5 7 s 12 s Dark recovery 88 s 3 11 s 26 s NPQ2 Light 200 s 10 10 s 20 s Dark recovery 390 s...

Page 21: ...details please refer to Oxborough K Baker N R 1997 Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non photochemical components calculation of qP and F...

Page 22: ...Page 22 Fig 8 NPQ Protocol...

Page 23: ...sities as shown in Table 2 below The visual representation of the LC1 and LC2 protocols is shown in Fig 9 and belowFig 10 of phases Phase duration Light intensities mol m 2 s 1 LC1 6 60s 10 20 50 100...

Page 24: ...Page 24 Fig 9 LC1 Protocol...

Page 25: ...Page 25 Fig 10 LC2 Protocol...

Page 26: ...te in the AquaPen Press SET to do the measurement The display should show value of 0 000 If the OD value is different than 0 000 repeat OD calibration again Remember that the calibration is specific t...

Page 27: ...nload to the PC page 44 Modes of Multiple measurement There are two modes of the multiple measurements in the AquaPen 1 The device is connected via USB to the computer The device measures according to...

Page 28: ...c shows the Main Menu first level Sub Menus and second level Sub Menus The blue color represents the Main Menu and its Options The yellow color represents the first level Sub Menus and their Options T...

Page 29: ...Page 29...

Page 30: ...Page 30...

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Page 32: ...Page 32...

Page 33: ...Page 33...

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Page 35: ...Page 35...

Page 36: ...se follow steps below in Fig 11 Fig 11 How to connect AquaPen with PC A connector on the AquaPen device B Portion of the USB cable with pins C E Position the cable horizontally and line up the green c...

Page 37: ...th documentation for more information Bluetooth must be switched on and be visible on both devices To pair the AquaPen with another Bluetooth device such as a computer ensure that Bluetooth is switche...

Page 38: ...da device 4 Selecting the AquaPen Select PSI AquaPen icon Click Next Fig 14 5 Starting the Pairing Process This step is different for old and new version of the AquaPen that are equipped with disparat...

Page 39: ...erification code is not important for the BT connection Select Next 6 Completing the AquaPen Pairing Select Close Fig 17 The Bluetooth pairing is now complete and the next step is to open the program...

Page 40: ...file SN txt which is included on the enclosed USB flash disk Please Note it is not possible to download data from the AquaPen device without software registration 3 Switch on the AquaPen and enable B...

Page 41: ...Erases data from the AquaPen memory Online Control Online control of AP device Attach GPS File Used for download data from GPS module active only in AquaPen version AP 100 MENU Setup Device ID Detects...

Page 42: ...red data are visualized in one graph i e the value of each new measurement is added to the currently used graph window If the box is not checked a new graph is opened for every new measurement 9 2 3 M...

Page 43: ...ically repeated measurement of one specific protocol over a time period The measurement result is represented by the values or graphs obtained in the specific data points The Multi interval represents...

Page 44: ...the previous settings can be started in this window Once the measurement is completed the data is saved to the device and can be downloaded to the PC later Measuring of OD 680nm and OD 720nm is activ...

Page 45: ...tion between the AquaPen and the PC needs to be established via USB cable or Bluetooth module see chapter 7 and 8 for details 2 Click the Download icon or select Device Download 3 Once the download is...

Page 46: ...Select data type to export Ft QY OJIP Fig 26 Selected only exports only one measurement that is selected by mouse otherwise it will export everything Source data exports raw data in case of OJIP poin...

Page 47: ...Page 47 Fig 26 Data export...

Page 48: ...ware update download all your data from the AquaPen memory to the computer 1 Starting Update Select Setup Update Firmware From File Fig 27 Fig 27 Firmware Update 2 Warning Select OK to start update Fi...

Page 49: ...ile 4 Finishing Upload Select OK to start uploading of the update Fig 30 The bottom bar indicates the upload progress Fig 31 Fig 31 Upload progress Press OK to finish upload Fig 32 Fig 30 Data loss wa...

Page 50: ...l appear followed by coordinate If the GPS module has difficulties mapping the coordinates a message stating GPS not locked will appear on the display It may be necessary to take the device outside in...

Page 51: ...Page 51 Fig 34 GPS coordinates...

Page 52: ...ses associated with the repair or installation of replacement parts at the customer s site The manufacturer repairs or replaces faulty instruments as quickly as possible the maximum time is one month...

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