Photon Systems Instruments AquaPen-C AP 110-C, Instruction Manual

Page: 14 / 52

Page | 14
5 G
ETTING STARTED
For more detailed information on steps of AquaPen operation please refer to chapter Error! Reference source not found. .
The device is powered by built in Li-Ion battery. Ensure that the battery if fully charged by plugging it into a PC via USB cable or the AC
outlet via USB adapter (not included) and the cable.
The AquaPen is controlled using two buttons:
•
Use the MENU key to scroll through sequential menu options shown on the digital display. And to turn the device off (hold for 3
sec).
•
Use the SET key to turn the device on (hold for 3 sec) and select a menu option based on cursor (>) position.
5.1 M
EASUREMENTS BASED ON FLUORESCENCE
5.1.1 P
ULSES DESCRIPTION AND SETTING
Flash pulse
This function serves for setting of measuring pulses intensity. The measuring pulses are weak light pulses, which are able to induce the
minimal
chlorophyll fluorescence (F
0
or F
t
). It takes only 30 µs and the maximum intensity is
3,000 µmol.m
-2
.s
-1
. It means 30 µs * 3,000 µmol.m
-2
.s
-1
= 0.09 µmol.m
-2
per pulse is the maximal intensity of the flash pulse.
Super pulse
This function serves for setting intensity of the saturating pulse. Saturating light pulse is able to induce maximum chlorophyll fluorescence
(F
m
). 100% of intensity represents approximately 3,000 µmol.m -2 .s -1 .
Actinic pulse
This function serves for setting intensity of actinic light. It is the ambient light in which the algae are growing. 100% of intensity equals
approximately 1,000 µmol.m
-2 .s -1 .
Pulses used in predefined protocols:
Measurements based on fluorescence Used pulses
Ft
Flash pulse
QY Flash pulse, Super pulse
OJIP Super pulse
NPQ protocols Flash pulse, Super pulse, Actinic pulse
Light Curves Flash pulse, Super pulse (Actinic pulse is preset)