Photometrics Cascade 128, User Manual, Page: 34 / 54

Chapter 3. Operating Features 23
Summary Table 5, below, summarizes typical Cascade:512F and 512B settings for two
types of applications and is provided as a setup aid. Actual settings will vary
based on the exact nature of your experiment design.
Application
Readout
Amplifier
(Port)
Readout
Speed
Multiplication
Gain
Conversion
Gain
(e-/ADU)
Readout
Mode
Kinetic
samples
(single
molecule
fluorescence
or tracking) Multiplication
(Port #1) 10 MHz
or
5 MHz Use as required
to obtain best
signal-to-noise
ratio (SNR).
Typical DAC
values are around
3000.
#2
(6 e-/ADU) Overlap
mode
(Frame
Transfer,
Pre-
Sequence)
Fixed samples
(bright field) Non-
multiplication
(Port #2) 1 MHz or
5 MHz Not Applicable #1
(4 e-/ADU)
Overlap
or Non-
overlap
Table 6. Application Type and Cascade:512F Setup Parameters