Photometrics Cascade 128, User Manual, Page: 31 / 54

20 Cascade User Manual
Note: The Cascade:128+ and Cascade:128 are only available with a multiplication port.
The Cascade:512F and the Cascade:512B are also available in "multiplication port only"
configuration. Please check the configuration for your system.
If your camera is configured with two readout amplifiers (ports), the software
automatically allows port selection. An example of a software dialogue to select
the ports is shown in
Figure 15 .
Figure 15. Software Selection of Readout Port
Note: As the user interface may be different, please refer to your software manual for
specific information on readout amplifier/port selection.
1. On-Chip Multiplication Amplifier (Port #1): Operation of
multiplication gain in the Cascade:512F, 512B, 128+, and 128 is similar to
that in Cascade:650. A setting of 0 (zero) results in unity gain and the
subsequent DAC settings are exponentially related to the multiplication
gain. Since the multiplication gain can be used to overcome the read
noise of the fast amplifier, this mode is most useful in applications
requiring low-light sensitivity at high frame rates (e.g., Single molecule
fluorescence, ion imaging, etc.).
Note: When used with a standard lens, Port #1 (multiplication port) produces an
image in the correct orientation. The first pixel is read out at the bottom left of
the array.
Readout Port
selection