5. Operation
Immobiline DryStrip pH gradient shapes
length (mm) of the strip
110
180
pH gradient
Shape
Shape
4-7
linear
linear
3-10
linear
linear
3-10
non-linear*
*The pH gradient is flattened in the pH range 5-7 to achieve a high resolving area in this part
of the gradient. The pH gradient is steeper in the pH ranges 3-5 and 7-10 than the linear pH
3-10 gradient. The linear 3-10 gradient gives improved resolution at pH values above 7.
Second dimension:
ExcelGel SDS polyacrylamide gradient gels are 0.5 mm thin precast
polyacrylamide gradient gels cast on plastic support film. The gels are
available in two different sizes with two different polyacrylamide gradients,
consisting of a stacking gel zone followed by the polyacrylamide gradient.
ExcelGel SDS gels
Gel
Gradient
Size (mm)
ExcelGel SDS
8-18
245 x 110
ExcelGel XL SDS
12-14
245 x 180
ExcelGel SDS buffer strips supply the gel with buffer ions and form a
discontinuous buffer system together with the buffer in the gel.
A summary of the 2-D method
Day 1: Rehydration of Immobiline DryStrip
Activities:
- Prepare solutions
- Wash reswelling cassette
- Assemble cassette with dry strips
- Fill Cassette with rehydration solution
Hands-on:
1 Hour
Run Time:
Overnight
Day 2: 1st Dimension IEF in Immobiline DryStrip
Activities:
- Prepare samples
- Remove strips from rehydration cassette
- Assemble running tray with strips and electrodes
- Overlay oil and load samples
- Turn on power supply
Hands-On:
1-2 Hours
Run Time:
Overnight
39
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
