5. Operation
Sample application
Apply 5-100 µl sample/well depending on gel type and sample
concentration.
Running conditions
Place the electrode holder with the EPH/IEF electrodes on the Multiphor II
unit. Align the electrodes so that they rest on the outer edges of the electrode
strips.
Connect the cables of the electrodes to the unit. Place the safety lid in
position. Connect the power supply. A low starting voltage for smooth
sample entry generally improves the result.
Recommended electrical settings for anodal electrophoresis with Native
Buffer Kit pH 8.9
Phase
Voltage
Current
Power
Time
(V)
(mA)
(W)
(min)
Pre-run
300
18
10
10
Run
900
50
30
60*
* Approximate time, or until the buffer front (dye marker Bromophenol Blue) reaches the
anodic wick.
Further information about the gels and running conditions are supplied with
the product.
Staining and preserving
For automated silver and coomassie staining of polyacrylamaida gels see
Protocol guide, Hoefer Automated Gel Stainer (80-6343-34).
CleanGel can be stained with most protein and DNA staining methods. The
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