5. Operation
Place the electrode holder on the electrophoresis unit in the shallow
depressions.
Align the EPH/IEF electrodes with the centre of the buffer strips by
loosening the clamping nuts and sliding the electrodes to the appropriate
position. Retighten the clamping nuts. Lift the electrode holder slightly and
reposition the supporting feet over the deep holes. Lower carefully, so that
the electrodes rest on the buffer strips. Connect the electrodes to the buffer
tank.
During electrophoresis, the socket on the bridging cable MUST be attached
to the pin connector at the front of the buffer tank as shown in the picture.
Connect the two electrodes to the buffer tank using the spring-loaded
cables on the electrodes.
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