PerkinElmer Spotlight 150, User Manual

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78 . Spotlight 150 User's Guide
3. Tighten the knurled thumb-nuts.
4. Move the microscope slide to a fresh area of tape.
5. Lower the crystal onto the tape.
6. Retract the crystal.
7. Look through the microscope at the impression of the crystal on the tape. Decide
whether the impression is in the center of the field of view.
8. If necessary, repeat steps 1 to 7 until the ATR objective is aligned.
Checking the Infrared Alignment
The following procedure allows you to determine the energy reaching the detector when the
ATR crystal is in place. If the crystal is aligned correctly, the energy level measured should be
at least 20% of the maximum energy measured in reflectance mode; see
Measuring the
Energy in Reflectance Mode
on page 75 for details.
1. Place the slide holding the reference mirror (supplied with the microscope) on the
Sample Stage.
2. Look at the video image on the Setup Microscope tab and focus the microscope on the
surface of the mirror.
There are usually dust particles or scratches on the surface that you can use to focus
on.
3. Lower the crystal onto the reference mirror.
4. Enter 100 in the Width and Height text boxes in the Apertures section of the Setup
Microscope tab.
5. Click on the Measurement bar and monitor the energy reaching the detector.
The energy level measured should be at least 20 of that measured in reflectance mode;
see
Measuring the Energy in Reflectance Mode
on page 75 for details.
6. Click .