PerkinElmer Lambda 465 Installation Instructions Manual Download Page 13 | Manualshive

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09931238D

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4.

Push the SET/ENT button again.

5.

Push the

button (OFF



ON).

6.

Press the SET/ENT button, MAN LED will blink.

7.

MAN LED will be off after a few minutes.

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Summary of Contents for Lambda 465

Page 1: ...rs See our website http perkinelmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4000 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the ...

Page 2: ...re 40 C Dimensions 310 W x 410 D x 275 H Weight 20 0 kg Coolant Volume 2 L N2 Gas Available 100 psi 6 9 bar Liquid Cooling System Magnetic Stirrer Control Available Emergency Power Off Switch Available Dimensions of Peltier Control Single Cell Physical Characteristic Specification Dimensions 101 W x 189 D x 133 H mm Weight 2 2 kg Temperature Accuracy 0 15 C Temperature Precision 0 1 C Temperature ...

Page 3: ... of the instrument Warning To prevent electrical shock do not open cover Refer all servicing to qualified personnel Avertissement Afin d éviter tout risque d électrocution ne pas enlever Warning For continued protection against electrical shock replace with same type and ratings of fuse Avertissement Pour assurer la protection contre le risque de choc électrique remplacez le fusible seulement avec...

Page 4: ...5 Peltier Control Single Cell a Interface connector between Peltier Single Cell Holder and Peltier Controller b Coolant Inlet Outlet Quick Coupler c N2 gas fitting d Interface slot for Temperature Probe e Cell Lifting knob f Temperature probe Figure 3 Temperature Probe Accessory Figure 4 Base Plate ...

Page 5: ...rm c Manual ON OFF button As the Manual ON OFF button is ON the temperature can be controlled manually by a Manual touch pad with display NOTE Manual operation is used only for the manufacturing process so it should be turned off during the measurement d N2 gas ON OFF button It is used for using N2 gas to remove the frost from the heating and cooling e Stirrer ON OFF button It is used for controll...

Page 6: ...emoving the air in the coolant hose f Buzzer It makes an alarm sound If there is any problem before the measurement the buzzer beeps 6 times every 10 sec If the malfunction occurs during the operation the buzzer beeps twice every 10 sec g Quick Coupler of Coolant lnlet Outlet h Quick Coupler of Coolant Drain i N2 Gas Inlet Outlet ports NOTE N2 gas is not always required Tube for N2 gas is not supp...

Page 7: ...use AC 250V T5AL 2ea Temperature Probe 1ea Installation 1 Place the Lambda 465 Peltier Controller and Peltier Control Single Cell in a location that is compatible with the required environmental conditions for the operation 2 Open the lid of the coolant inlet on top of the Peltier Controller Figure 9 Lid of coolant inlet 3 Fill up the liquid coolant using the funnel CAUTION ATTENTION Make sure the...

Page 8: ...ailable e g liquid coolant including Distilled Water 70 wt Propylene Glycol 27 wt Additives 3 wt Le liquide de refroidissement doit être utilisé comme réfrigérant N utilisez jamais d eau eau du robinet eau déminéralisée etc L eau risque en effet de corroder les composants du contrôleur Peltier et de réduire les performances de ce dernier Utilisez un liquide de refroidissement du commerce de qualit...

Page 9: ...ntrol Single Cell with the Phillips round screw Figure 11 Placing the plate at the bottom of the sample compartment 7 Connect the accessory interface cable of the Peltier Controller to the interface connector of the Peltier Control Single Cell Figure 12 Connecting the accessory interface cable of the Peltier Controller to the interface connector ...

Page 10: ... Figure 13 Connecting the coolant inlet outlet and N2 gas tube 9 Connect the temperature probe to the interface slot of the Peltier Control Single Cell Figure 14 Connecting the temperature probe 10 Connect the Peltier Controller with the PC via the RS 232 or USB to RS 232 cable See Installing the USB to RS 232 driver on page 15 when you use the USB to RS 232 cable Figure 15 Connecting the Peltier ...

Page 11: ...er of the Peltier Controller and also during the operation since it could make a trouble for the communication between Lambda 465 and Peltier Controller Le bouton d actionnement manuel de la ventilation doit être désactivé avant que le contrôleur Peltier ne soit mis sous tension et pendant le fonctionnement de ce dernier afin d éviter qu une erreur de communication entre le Lambda 465 et le contrô...

Page 12: ...e the existing peltier cell holder to the other one you should proceed AUTO TUNING set up to minimize the temperature fluctuation at the target temperature 1 Push the Manual button on the control panel of the peltier controller then button light is on 2 Set any SP temperature between 30 37 C using the button 3 Press and hold the SET ENT button until shown G AT ...

Page 13: ...09931238D 13 4 Push the SET ENT button again 5 Push the button OFF ON 6 Press the SET ENT button MAN LED will blink 7 MAN LED will be off after a few minutes ...

Page 14: ...s les fonctions et contrôlez soigneusement l état du contrôleur Peltier Ensuite tournez à nouveau l interrupteur vers la droite une fois la situation rétablie afin de restaurer les fonctions Figure 19 Emergency switch 14 Turn off the power after the experiment How to drain the coolant from Peltier Controller a Push the Air Vent Manual button to be switched on the rear panel of Peltier Controller b...

Page 15: ...drive properly according to the following procedure 1 Turn on the computer and the Peltier controller 2 Connect the USB to RS 232 cable between the computer and the Peltier controller 3 Select C UV Lab USB Drivers Lambda 465 USB2 folder 4 Double click USBCom CDM_20828 5 Select Extract 6 Select Next ...

Page 16: ...pleted successfully select Finish 8 Change the USB serial ports after finishing driver setup See the following section Setting USB to RS 232 port Change the port setting as follow when communication is not connected by using the USB to RS 232 convert 1 Select My Computer Properties ...

Page 17: ...2 Select Device Manager 3 Select Ports COM LPT to expand the listing These are the devices currently connected to the COM ports USB Serial Port COMx is visible when the driver installation is completed successfully ...

Page 18: ...09931238D 18 4 Double click on the USB Serial Port COMx of the Ports COM LPT section 5 Select the Port Settings tab and select the Advanced button ...

Page 19: ...owing screen 7 Select OK after checking the changed parameter values 8 If the Peltier controller fails to communicate with the PC change the COM Port Number in the following steps 9 Open the Advanced Setting for COMx window following steps 1 to 6 in this procedure ...

Page 20: ...number list to expand it and change the COM port number another one which is not in use from COM 1 to COM 10 11 Make sure that the changed COM Port Number is applied and select OK 12 After the port setting is changed restart the computer ...

Page 21: ...software When the window below is appeared select Experiment Type and click OK 2 The following dialog box will be displayed 3 Setup test parameters as follows a Edit Heating Cooling Rate C min Set the heating cooling temperature rate in each temperature range Sample is measured whenever it reaches the set temperature by the rate C min and the sample is also measured at the Start Temperature and th...

Page 22: ...rt Temperature Enter the start temperature for the calculation e Calculation End Temperature Enter the end temperature for the calculation NOTE Calculation Start Temperature and Calculation End Temperature must be entered within Start Temperature and End temperature range 4 Select Wavelength Range and enter the wavelength range for the measurement 5 After setting parameters for Experiment Setup Ba...

Page 23: ...nd error range as 1 C then the monitoring temperature is set as the temperature of cell block In this case the measurement will start when the temperature of the cell block reaches between 19 C and 21 C NOTE When selecting probe for Monitor in Collect Temperature box the slower the real heating speed may be the smaller the set Error range C is Collect Temperature a Monitor Select which temperature...

Page 24: ... 10 Insert the blank solution into the cell holder Select Blank and then the blank is measured when it reaches the set Start Temperature NOTE To monitor the probe temperature the probes should be immersed in the sample or to use the block temperature for monitoring the cell lid should be closed tightly 11 Remove the blank solution from the sample cell holder and insert the sample solution into the...

Page 25: ...he elapsed time to reach the sampling temperature j Status Shows the status of experimental progress k Error Range C Shows the temperature tolerance between the sampling temperature and monitored temperature The measurement will be made only when the temperature tolerance reaches within the set error range NOTE To pull out the cell easily use the cell lifting knob Figure 20 Pull out the cell 12 Af...

Page 26: ...lick on Insert and Delete to edit the temperature range and the rate Start Temperature C Enter the start temperature for the measurement End Temperature C Enter the end temperature for the measurement Rate C min Enter the heating cooling rate b Holding Time min Enter the holding time before sample measurement after it reaches the each set temperature c Volume Correction If the volume correction is...

Page 27: ...ne Cytosine base pairs is G C 2 44 Tm 81 5 16 66 log M 500 K Where M is the molarity of salt in mol l K is the DNA base pair length If a DNA base pair length K is entered as 0 then the equation becomes G C 2 44 Tm 81 5 16 66 log M g Calculation Start Temperature C Enter the start temperature for calculating the Tm value using the selected method h Calculation End Temperature C Enter the end temper...

Page 28: ...ll start when the temperature tolerance reaches within the set Error Range Example Set the starting temperature as 30 C and error range as 0 5 C then the monitoring temperature is set as the temperature of cell block In this case the measurement will start when the temperature of cell block reaches between 29 5 C and 30 5 C NOTE When selecting probe for Monitor in Collect Temperature box the slowe...

Page 29: ... up or cooling down to the set temperature in the Setup Single Cell Peltier 10 Insert the blank solution into the cell holder Select Blank and then the blank is measured when it reaches the set start temperature NOTE To monitor the probe temperature the probes should be immersed in the sample or to use the block temperature for monitoring the cell lid should be closed tightly 11 Remove the blank s...

Page 30: ...erature is shown out of Block Probe 1 and Probe 2 h Hold time min Shows the set holding time i Elapsed time m s Shows the elapsed time to reach the sampling temperature j Status Shows the status of experimental progress k Error Range C Shows the temperature tolerance between the sampling temperature and monitored temperature The measurement will be made only when the temperature tolerance reaches ...

Page 31: ...ear panel of the instrument 4 Carefully open the compartment latch where the fuse is located 5 Disconnect the fuse 6 Replace with a new T5AL fuse AC 250V One spare is contained in the power module 7 Close the compartment door 8 Plug in the instrument and turn on On Line LED is not lit on 1 Check whether the communication cable is connected tightly 2 Check whether the Air Vent Manual button is swit...

Page 32: ...f the Flow Sensor LED is continuously lit on with an alarm sounding contact your PerkinElmer service representative Pump LED is lit on with an alarm sound 1 Check whether the tubing is bent or whether it is connected correctly and then restart the Peltier Controller 2 Push the Air Vent Manual button to be switched on and check if coolant flows properly for about 1 minute after the error occurs If ...

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