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PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi, Installation Instructions Manual

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PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi Installation Instructions Manual Download Page 3

09931463A 

3

 

Safety Warnings  

 

WARNING 

 

AVERTISSEMENT 

 When this label is attached to an instrument it means refer to the 

manual. 

 

 

Lorsque cette étiquette est attachée à un instrument, il est 

nécessaire de voir le manuel. 

 

 

WARNING 

 

AVERTISSEMENT 

There is risk of receiving a fatal electric shock if the fuses are 

replaced with the power cord connected. 

 

 

Il y a un risque d’électrocution si les fusibles sont remplacés tandis 

que le cordon d'alimentation est encore branché. 

See the following figure for the location of warning labels on the back of the instrument: 

 

 

 

Warning 

FUSE 

 

Avertissement 
FUSE 

Warning 

To prevent electrical shock,  

do not open cover. 

Refer all servicing to qualified 

personnel. 

 

Avertissement 
Afin d’éviter tout risque 
d’électrocution ne pas enlever ce 
couvercle. 
Faire appel au service Après-Vente. 

Warning 

For continued protection against electrical 

shock, replace with same type and ratings of 
fuse. 

 

Avertissement 

Pour assurer la protection contre le risque de 

choc électrique, remplacez le fusible 

seulement avec le même type et la même 
valeur nominale. 

 

Summary of Contents for Lambda 365 Peltier Temp Ctrl Unit Multi

Page 1: ...art numbers See our website http perkinelmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4000 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outsid...

Page 2: ...able 1 5 bar N2 Gas Available 1 bar Liquid Cooling System Magnetic Stirrer Control Available 6 Position Peltier Controlled Cell Changer with and without heated reference Physical Characteristic Specification The number of Sample Cells 6 The number of Reference Cell 1 with or without heated reference Dimensions 134 5 W x 384 D x 142 5 H mm 5 3 W x 15 1 D x 5 6 H in Weight With heated reference 3 4 ...

Page 3: ... d alimentation est encore branché See the following figure for the location of warning labels on the back of the instrument Warning FUSE Avertissement FUSE Warning To prevent electrical shock do not open cover Refer all servicing to qualified personnel Avertissement Afin d éviter tout risque d électrocution ne pas enlever ce couvercle Faire appel au service Après Vente Warning For continued prote...

Page 4: ...or Coolant 1 each Waste Hose for Coolant 1 each Hose for Coolant circulation 1 each Phillips round h d screw with washer 2 each Macro Cell with PTFE Stopper 2 each NOTE Peltier Temp Ctrl Unit Multil includes two 2 macro cells in the standard configuration Additionally required macro cells for the 6 Position Peltier Controlled Cell Changer must be purchased separately Description 6 Position Peltier...

Page 5: ...k Coupler d Interface slot for Temperature Probe e Cell Lifting knob for sample cells f Cell Lifting knob for reference cell g Phillips round headscrew with washer Used to fix a cell holder a base plate or a front plate for the Lambda 365 Spare screws 2each are enveloped with the accessory Figure 3 Phillips round headscrew with washer M4 12L h Macro Cell with PTFE stopper Figure 4 Macro Cell with ...

Page 6: ...e operation of Coolant Circulation Fan Pump etc If there is any problem in the components Red LED will flash with beep alarm d Display pad Display temperature of sample and reference block e Stirrer ON OFF button It is used for controlling of the stirring f AC Power Main power ON OFF Switch symbol IEC 60417 5268 2002 10 In position of a bi stable push control IEC 60417 5269 2002 10 Out position of...

Page 7: ... NOTE Air Vent Manual button is recommended to be manipulated by service engineer b Buzzer It makes an alarm sound If there is any problem before the measurement or the malfunction occurs during the operation Buzzer beeps c USB port d Fuse AC socket Fuse Holder e Interface Interface cable is connected with the 6 Position Peltier Controlled Cell Changer f Quick Coupler of Coolant lnlet Outlet g Qui...

Page 8: ...1 in a location that is compatible with the required environmental conditions for the operation 2 Connect the hose for coolant circulation to the Peltier Temperature Controller 201 Figure 8 Connect the hose for coolant circulation 3 Connect the power cord to the Peltier Temperature Controller 201 Figure 9 Connect the power cord 4 Remove the Phillips screws 4 each on the Air Vent Manual cover using...

Page 9: ...on on Figure 11 Push the button 6 Turn on the AC power switch of the Peltier Temperature Controller 201 Figure 12 Turn on the AC power switch 7 Check if the LEDs of LOW LEVEL and FLOW SENSOR are lit on Figure 13 LEDs of LOW LEVEL and FLOW SENSOR ...

Page 10: ...rigerant the PeltierTemperature Controller 201 s components might be corroded and its performance may be deteriorated Use any kind of liquid coolant of normal grade that is available e g liquid coolant including Distilled Water 70 wt Propylene Glycol 27 wt Additives 3 wt Le liquide de refroidissement doit être utilisé comme réfrigérant N utilisez jamais d eau eau du robinet eau déminéralisée etc L...

Page 11: ... Fasten Phillips screws 4 each on the Air Vent Manual button cover using Phillips screwdriver 15 Install the 6 Position Peltier Controlled Cell Changer referring to the Installation section page 13 How to Drain the Coolant 1 Turn off the Peltier Temperature Controller 201 2 Remove the Phillips screws 4 each on the Air Vent Manual cover using Phillips screwdriver Figure 17 Removing Phillips screws ...

Page 12: ...ure 20 Turn on the AC power switch 6 Connect the waste hose to the Coolant Drain port on the rear panel Figure 21 Connect the waste hose NOTE The Coolant flows out as soon as the waste hose is connected so the other side of the waste hose should be placed into the waste basket before connected 7 The coolant will be drained automatically ...

Page 13: ...on 1 Prepare the Lambda 365 and Peltier Temperature Controller 201 in a location that is compatible with the required environmental conditions for the operation 2 Connect the power cord and communication cable of the Lambda 365 DO NOT turn on the power of the instrument 3 Remove the two Phillips round head screws with washer M4 12L to disassemble the existing cell holder and base plate Figure 23 L...

Page 14: ...ell Changerin the sample compartment Figure 25 Inserting the 6 Position Peltier Controlled Cell Changer 6 Gently press the 6 Position Peltier Controlled Cell Changer to connect the communication port male under the bottom of the 6 Position Peltier Controlled Cell Changer to the port female in the sample compartment Figure 26 Connecting the communication ports ...

Page 15: ...e cable of the Peltier Temperature Controller 201 to the interface connector of the 6 Position Peltier Controlled Cell Changer Figure 28 Connecting the accessory interface cable 9 Disconnect the hose for coolant circulation Figure 29 Disconnecting the hose for coolant circulation NOTE If you disconnect the hose the remaining coolant in the hose may flow out of the hose Be careful not to touch the ...

Page 16: ...necting the coolant inlet outlet and N2 gas fitting NOTE N2 gas is not always required If no gas is applied user does not have to connect N2 gas tube 11 Open the lid of Interface slot for Temperature Probe by hand Figure 31 Opening the lid of Interface slot 12 Connect the temperature probe to the Interface slot for temperature probe Figure 32 Connecting the temperature probe ...

Page 17: ...probe Figure 33 Stuffing the probe line into the cell compartment 14 Connect the Peltier Temperature Controller 201 with the PC via the USB cable Figure 34 Connecting the Peltier Temperature Controller 201 with the PC 15 Connect the power cord to the Peltier Temperature Controller 201 Figure 35 Connecting the power cord ...

Page 18: ...ivé avant que le contrôleur Peltier ne soit mis sous tension et pendant le fonctionnement de ce dernier afin d éviter qu une erreur de communication entre le Lambda 365 et le contrôleur Peltier ne se produise Figure 36 Turn on the AC power switch 17 Check that the Power LED is on as a blue light Figure 37 Location of Power LED NOTE Peltier Temperature controller 201 can be compatible with various ...

Page 19: ...when installing the Lambda 365 and UV Express software user does not need to install it again When the communication by the USB is not established change the port setting as follows 1 In Windows 7 select My Computer Properties In Windows 10 press Windows Pause Break on the keyboard 2 Select Device Manager ...

Page 20: ... are the devices currently connected to the COM ports USB Serial Port COMx is listed when the driver installation is completed successfully 4 Double click on the USB Serial Port COMx of the Ports COM LPT 5 Select the Port Settings tab and select the Advanced button ...

Page 21: ... 7 Select OK after checking the changed parameter values 8 If the Peltier Temperature Controller 201 fails to communicate with the PC change the COM Port Number by the following steps 9 Open Advanced Setting for COMx window again by repeating the steps 1 to 6 ...

Page 22: ...umber list to expand it and change the COM port number to another one which is not in use from COM 1 to COM 10 11 Make sure that the changed COM Port Number is applied and select OK 12 After the port setting is changed restart the computer ...

Page 23: ...the System Self Test after warming up the system for at least 20 minutes 3 Double click on the UV Express folder and select Thermal Denaturation mode 4 Select OK after finishing the System Self Test 5 Select New to open a new window and select OK NOTE When the 6 Position Peltier Controlled Cell Changer is connected for the first time you need to correct the cell position Calibrate the beam positio...

Page 24: ...e pairs is G C 2 44 Tm 81 5 16 66 log M 500 K Where M is the molarities of salt in mol L K is the DNA base pair length G C will be calculated with estimated Tm and input values Molarities M and length of DNA K f DNA Pair Length K Enter the DNA base pair length This value will be used calculating G C g Calculation Start Temperature C Enter the start temperature for calculating the Tm value h Calcul...

Page 25: ...01 see section Setting USB Serial Port page 19 if the Method Setup is not activated 9 The Peltier window will be shown Select the Multi Cell Peltier tab and set parameters according to the experiment conditions Multi Cell holder a Available Cells Indicate cells that are available for measurement In the case of 6 Position Peltier Controlled Cell Changer six cells are available ...

Page 26: ...e for the measurement j Interval C Enter the measurement interval temperature For instance if you enter 5 C the sample will be measured from start temperature to end temperature every 5 C k Rate C min Enter the heating or cooling temperature rate in each temperature range NOTE The rate has to be set under or equal to interval value l Hold min Enter the holding time For example if you set 1 min mea...

Page 27: ...be measured when it reaches the set Start Temperature NOTE To monitor the probe temperature the probes should be immersed in the sample or to use the block temperature for monitoring the cell lid should be closed tightly a Start Temp C The starting temperature of experiment b SP Temp C The set temperature to go to the next measurement temp c Block Temp C The temperature of Cell Block d Probe 1 Tem...

Page 28: ...nt is finished the results are displayed in the result window Save or print the results as required Other Measurement Modes NOTE Peltier Temperature Control Unit is available in all methods however unlike Thermal Denaturation the measurement is only performed with isothermal state 1 Install the 6 Position Peliter Controlled Cell Changer referring to section Installation page 13 2 Close the sample ...

Page 29: ... 33 6 Open the method in main menu or click the mode icon on the left side of the main window Set parameters for Experiment NOTE If you want to know about the meaning of each parameter of method tabs and other modes more detail please refer to X Measurement Modes of the UV Express Software User Guide 7 After setting parameters for Experiment Lamp and Kinetics click Accessory tab Select Multi Cell ...

Page 30: ...ed positions If not zero baseline will be only measured at the 1st cell position among the using cells Multi Baseline is only available at the Scan and Scanning Kinetics mode NOTE To prevent inadequate baseline zero measurement because of the difference in transmittance among cuvettes or any other reasons Multi Baseline Multi Zero measurement is recommended d Temperature C Enter the returning temp...

Page 31: ...e parameter setup select Apply and OK Then the LED of ON LINE is turned on it will start heating up or cooling down to the set temperature in the Setup Multi Cell Peltier tab 11 Insert blank solutions into the both reference and sample cell holder Select Baseline or Zero and then baseline or zero will be measured when it reaches the set Temperature NOTE To monitor the probe temperature the probes ...

Page 32: ...ill start when the temperature tolerance reaches within the set Error Range h Hold Time It shows the set holding time i Elapsed Time s It shows the elapsed time to reach the sampling temperature j Status It shows the status of experimental progress 12 Remove blank solution from sample cell holder and insert sample solution into the sample cell holder Select Sample then the measurement will be star...

Page 33: ...trolled Cell Changerwhen the Multi Cell is installed for the first time or beam position is incorrect 1 Execute one measurement mode of UV Express software Scan for example and open the Method Setup of the Multi Cell Peltier 2 Select the Calibration tab in the Peltier setup The following dialog box will appear ...

Page 34: ...p operation during Multi Cell calibration Save Result It is used to save the calibrated result Reset Multicell Move to 0 step of Multi Cell position NOTE Although eight positions steps are displayed only the first six 6 positions are used for 6 Position Peltier Controlled Cell Changer The last two positions remain 0 steps 4 Click Reset Multicell to format the Multi Cell steps Click OK 5 Click Mult...

Page 35: ...libration will start The current process of calibration will be shown in the main window 7 When calibration is finished the following box appears Click OK 8 Click Save Result to save the result If the following message box appears click OK ...

Page 36: ... minimize the temperature fluctuation at the target temperature 1 Connect the accessory interface cable of the Peltier Temperature Controller 201 to the connector of the peltier cell changer 2 Turn on the AC power switch of the Lambda 365 and the Peltier Temperature Controller 201 3 Insert UV Express installation CD into the CD ROM drive Select Computer CD Drive Double Click on Peltier Auto Calibr...

Page 37: ...alimentation est encore branché 2 Turn off and unplug the instrument 3 Locate the fuse cover on the left rear panel of the instrument 4 Carefully open the compartment latch where the fuse is located 5 Disconnect the fuse 6 Replace with a new T5AL fuse AC 250V One spare is contained in the power module 7 Close the compartment door 8 Plug in the instrument and turn on On Line LED is not lit on 1 Che...

Page 38: ... sound 1 Tunn off the power of the peltier temperature controller 201 2 Remove Phillips screws 4 each on the Air Vent Manual button cover using Phillips screwdriver 3 Push the Air Vent Manual button on 4 Restart the Peltier Temperature Controller 201 and check the coolant level and if it is lower the L low mark fill up the coolant more ...

Page 39: ...urn off the AC power switch of the Peltier Temperature Controller 201 8 Push the Air Vent Manual button off 9 Fasten Phillips screws 4 each on the Air Vent Manual button cover using Phillips screwdriver FLOW SENSOR and PUMP LED is lit on with an alarm sound 1 Turn off the power of the Peltier Temperature Controller 201 ...

Page 40: ...nd check if coolant flows properly for about one minute 6 Check if the LED blinks of FLOW SENSOR and PUMP stop 7 Close the lid of the coolant inlet 8 Turn off the AC power switch of the Peltier Temperature Controller 201 9 Push the Air Vent Manual button off 10 Fasten Phillips screws 4 each on the Air Vent Manual button cover using Phillips screwdriver 11 If the FLOW SENSOR and PUMP LED is continu...

Page 41: ...l holder s hole 2 If the intensity value is still low after the recalibration the lamps of the light source may be deteriorated and need replacing Contact your PerkinElmer service representative When 6 Position Peltier Controlled Cell Changerdoes not reset 1 If reset is failed the photo interrupt switch inside the 6 Position Peltier Controlled Cell Changerneeds replacing Contact your PerkinElmer s...

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