Perkin-Elmer FL 6500 Installation Instructions Manual Download Page 18 | Manualshive

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Summary of Contents for FL 6500

Page 1: ...part numbers See our website http perkinelmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4000 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outsi...

Page 2: ...430 5 D x 331 H Microplate Reader Cover Weight Kg 4 25 Available Microplates Physical Characteristic Specifications The number of Wells Default 96 or 384 and customize available Microplate Size mm 85 4 x 127 6 Configuration of the Microplate Reader Microplate Reader Figure 2 Microplate Reader a Microplate fixing jig b X and Y axis stage c Microplate Reader fixing bolt d Collimator Lens Holder Emis...

Page 3: ... Figure 3 Microplate Reader Cover Two cradles for the Microplate Reader Figure 4 Cradles Installation 1 Prepare the FL 6500 8500 Fluorescence Spectrometer to install this accessory 2 Connect the instrument power cord and the communication cable 3 Loosen the accessory fixing bolt and remove the existing sample accessory Figure 5 Loosening the accessory fixing bolt ...

Page 4: ...cover of the FL 6500 8500 using a screwdriver Figure 7 Front Cover 6 Place the front cover of Microplate Reader and fasten the three bolts using a screwdriver Figure 8 Front Cover of Microplate Reader 7 Undo the four screws which attach the Hinge Guide to the sample compartment lid Figure 9 Remove the Hinge Guide ...

Page 5: ...0 8500 Figure 10 Remove the sample compartment lid 9 Tighten the four screws that secure the cover of the plate reader to the FL 6500 8500 Figure 11 Cover for the Microplate Reader 10 Attach the Microplate Reader cradles to the sample compartment Figure 12 Cradles of the Microplate Reader ...

Page 6: ...4 bolts 12 Connect the M1 and M2 cables of the Microplate Reader into the accessory port Figure 14 Connect M1 and M2 cables 13 Fit the groove hole of the Microplate Reader into the position of the cradles If positioned well the pogo pin will fit also Figure 15 Install the Microplate Reader ...

Page 7: ...of Microplate Reader 15 Close the Microplate Reader cover and then lock the latch at the left and right side of the front cover Figure 17 Lock the latches NOTE Sample loading can be done by opening the upper door of the Microplate Reader Figure 18 Upper door of the Microplate Reader ...

Page 8: ... Accessory icon 3 The following window will appear Select the plate you want to use 4 Enter the required parameters on each tab if you know the whole plate information List Function Plate Setting Select the type of well plate Default is 96 or 384 wells Customized setting is available Column Count Enter the value for the number of columns ...

Page 9: ...eload Open the previously saved parameters Save Save the setting parameters Manual Alignment Refer to the Manual Alignment chapter Show Dimensions If you check Show Dimensions it shows the range of each parameter 5 Click Save 6 Click the Lamp On button in the Operation to turn on the lamp Enter the A1 and click Move to button 7 If the A1 position is not correct Go to the next Manual Alignment sect...

Page 10: ...ation or A1 position is not correct proceed the manual alignment 1 Prepare a micro plate and Scotch MagicTM Tape Attach the magic tape onto the A1 and H12 96 well or P24 384 well area as shown below 2 Load the micro plate 3 Click Manual Alignment in the Plate Information ...

Page 11: ...ntrol Use the arrows to align the probe Each time you click on one of the arrows the probe is moved in the indicated direction The distance the probe is moved is determined by the alignment distance Operation List Function Move to Start Position Move the micro plate to the start position Turn on Lamp Turn on the lamp Turn off Lamp Turn off the lamp 5 Enter the Row and Column counts in the Step 1 T...

Page 12: ...931528A 12 7 Click Done in Step 2 8 Align the beam to the centre of A1 using the Movement Control icons and click Done 9 Align the beam to the centre of H12 using the Movement Control icons and click Done ...

Page 13: ...10 Align the beam to the edge of H12 using the Movement Control icons and click Done 11 Click Finish to save the plate information and close the window 12 You can see that the Plate Information tab has been changed ...

Page 14: ...rement 1 Open the Spectrum FL software and select a Microplate Reader mode in the Method Setup 2 In the Data Collection tab set the measurement parameters in turn NOTE For more detail of method refer to Spectrum FL Software Users Guide 3 When you click the Excitation of the Instrument Setting enter the number of the wavelength for measurement in the Measured Num The ID in the Excitation Setup is g...

Page 15: ...ll to set a measurement point the measurement point is automatically set to the center of the well Cycle Number This is the number of measurement cycle Time Interval The time interval is the time between last measurement of current cycle and the 1st measurement of next cycle Before the next cycle measurement a message window will pop up as shown below Click Skip to measure immediately b When you s...

Page 16: ...veraged Sum If selected multiple reads for a given well are summed integrated The results spreadsheet is updated when all readings are performed for a given well Cycle Number This is the number of measurement cycle Time Interval The time interval is the time between last measurement of the current cycle and the first measurement of next cycle Before the next cycle measurement a message window will...

Page 17: ...ellation Click the selected position again Area selection Drag the mouse Area cancellation Click the mouse outside the plate anywhere 8 Click Save to save the method after setting up the parameters 9 Prepare a Microplate filled with the sample for measurement 10 Load the Microplate 11 Select the Run icon 12 Input the sample name and select OK 13 Save or print the data if required ...

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