CyFlow
®
Cube Instrument Operating Manual
Rev018_2012-01-27
9/36
Example of a histogram
Example of a dotplot
Peaks
Number of events
Threshold of
lower limit
(L-L)
Linear
representation
of channels
Log representation
for fluorescence
A 2D dotplot presents correlated data over 2
dimensions. In the image on the right a sample of
leukocytes (after lyse of the red blood cells) is
plotted with their relative light scattering (SSC)
property against the intensity of the CD45 antigen.
The Z value represents the number of events that
have the same coordinates. 1 event will be
represented by a black point; if 10 events have
the same coordinates, the point representing
them will be grey. It will be red if more than 20
points are overlaying one another. The Z scale is
dynamic and will adapt during the measurement
to a scale of 1 (black) to the maximum overlaying
event coordinate colour coded in red.
A histogram represents a distribution of measured
signals (events) over 1 dimension. Data can be
presented on the dimensions of relative particle
size or optical particle structure (Forward Scatter
(FCS) or Side Scatter (SSC)), resp. or on their
relative fluorescence intensities in different light
colours (fluorescence parameters FL1 to FL6).
In this example, the dimension represented is the
relative size (FSC) on a logarithmic scale in X,
and the number of events on a linear scale in Y.
Two peaks are visible.
