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PALM

®

 MicroBeam User Manual - 0202 Z

5

Important Basic Information

Legal Notes

1 Important Basic Information 

Congratulations! With your new PALM

®

 Micro-

Beam you obtained the State-of-the-Art Technol-
ogy in microdissection and micromanipulation. The 
PALM

®

 MicroBeam is a high precision instrument, 

which allows to perform microdissection in nearly 
every kind of research as well as in routine exam-
ination with a high throughput.
To meet a very high safety standard and to use all 

the benefits of your PALM

®

 MicroBeam you should 

read this user´s manual carefully and observe all 
instructions given in this manual. Please follow 

also the instructions for the single components of 
your PALM

®

 MicroBeam especially for the micro-

scope, the fluorescence attachment (option), the 

video camera and the laser. P.A.L.M. Microlaser 
Technologies will not be responsible for mistakes 
and possible consequences caused by non-obser-

vance of the user´s manual.
Please consider also the manuals provided for your 
periphery devices!

1.1 Legal Notes

P.A.L.M. Microlaser Technologies AG is not liable 
for damages resulting from the fact that this user 
manual was not or only partly observed.
The warranty extinguishes in case the customer 

opens the laser housing or uses the device for 
other than the specified purposes.
If used parts are replaced by similar parts which 
are, however, not identical to those recommended 

by the manufacturer in this manual, P.A.L.M. 
Microlaser Technologies AG is only liable if the 
product supplied has already been faulty before 

the parts were replaced.
For details concerning the manufacturers war-
ranty, please refer to the contractual conditions.

1.2 Conventions for this User Manual

Special markings on the left margin point out 
information you should observe by all means:

1.3 How You Can Contact Us

Warning

This sign warns you of possible most serious, 

irreversible injuries to persons even going as 
far as to death.

Caution

This sign signalizes possible moderate personal 
damage and possible damage of the device.

Notice

This sign signalizes that information is given 
demanding your special attention.

Info:

This indicates tips on correct use and offers 
useful notes for getting the best use of the soft-
ware.

Business hours

9 h - 15.00 h, Mo - Fr

Phone

+49 (0) 81 58 99 71-100

Fax

+49 (0) 81 58 99 71-249

E-mail

Info@palm-microlaser.com
support@palm-microlaser.com

Service Address P.A.L.M. Microlaser Technolo-

gies AG

Am Neuland 9 + 12
82347 Bernried
Germany

Homepage

www.palm-microlaser.com

Summary of Contents for MicroBeam

Page 1: ...PALM MicroBeam User Manual PALM MicroBeam User Manual 0202 Z ...

Page 2: ...o responsibility for any errors that may appear Printed June 2002 Second Edition The following technologies have been patented by P A L M Microlaser Technologies Laser catapult technology Laser Pressure Catapulting LPCpat Patents US 5 998 129 EP 879408 B1 and others Three dimensional laser beam positioning system Patents US 5 689 109 EP 679325 B1 and others Additional patents pending P A L M Micro...

Page 3: ...ing Risks Infectious Material 9 4 7 4 Remaining Risks EMV 10 4 7 5 Remaining Risks Fire Extinction 10 4 8 How to Ensure a Safe and Ecological Disposal 10 5 The PALM MicroBeam Survey 11 5 1 PALM ControlUnit 12 5 2 Laser Interface 12 5 3 Laser 13 5 4 Microscope Adapter 13 5 5 Microscope Zeiss Axiovert 200 14 5 5 1 Fluorescence attachment 15 5 6 PALM RoboStage 15 5 7 PALM CapMover 16 5 7 1 Control Ap...

Page 4: ...1 Simultaneous Fluorescence Observation 22 8 Trouble Shooting List 23 8 1 Laser is not cutting 23 8 2 No video image 24 8 3 Shift between drawing and cutting 24 9 Maintenance of the PALM MicroBeam 25 9 1 Laser 25 9 2 Microscope 25 9 2 1 Cleaning of the Optical Parts 25 9 2 2 Cleaning When Actually Dirty 25 10 Technical Data 26 10 1 Electricity 26 10 2 Nitrogen Laser 26 ...

Page 5: ...nly partly observed The warranty extinguishes in case the customer opens the laser housing or uses the device for other than the specified purposes If used parts are replaced by similar parts which are however not identical to those recommended by the manufacturer in this manual P A L M Microlaser Technologies AG is only liable if the product supplied has already been faulty before the parts were ...

Page 6: ...tem should be installed in a room which is adequately large about 6 square meters and well aired There should be a free space available about 90cm above the table and all shelves wall cup boards etc should be removed Figure 1 Dimensions Nature of the floor vibration free if not spe cial working table recommended and even Load carrying capacity for steel or granite table 400 kg Minimum space needed...

Page 7: ...croBeam is a laser device of Class 1 M with no special safety requirements if operated according to the instructions given in this user man ual Built in is a Laser of class 3B It bears a potential risk A direct or reflected beam must not strike the unprotected eye do not look directly into the beam In service case the device has to be operated by the service staff or an authorized representative o...

Page 8: ...ing safety markings and the type plate exist and are legible Replace miss ing or illegible safety markings or type plate Figure 2 Safety Markings and Type Plate Caution The safety markings on the device must always be clearly visible and legible and must strictly be observed P A L M MicrolaserTechnologies Class M1 Identification label ...

Page 9: ... 26 4 7 Pay Attention to Remaining Risks and Preventive Measures 4 7 1 Remaining Risks and Protective Measures Laser Remaining risks The laser works with invisible light When the casing is open the laser pertains to the laser protection class 3B The high power radiation leads to dimmed sight of the eye The casing must always kept closed and locked and may only be opened by the ser vice staff of P ...

Page 10: ...adiation in the same room where the PALM MicroBeam is placed 4 7 5 Remaining Risks Fire Extinction To extinguish fire use sand carbon dioxide or fire extinguishing powder Never ever use water because of the electric dangers 4 8 How to Ensure a Safe and Ecological Disposal Dispose the following materials in an environmen tally friendly way Microscope lamps with incorporated bulb or individual mercu...

Page 11: ... the following chapters Figure 3 Survey of the PALM Micro Beam P A L M MicrolaserTechnologies P A L M MicrolaserTechnologies 1 2 3 4 5 6 7 8 12 10 11 13 14 15 16 9 1 microscope 2 CCD video camera 3 lmicroscope stage 4 CapMover option 5 filter wheel option 6 fluorescence illumination option 7 laser interface and laser 8 control unit 9 monitor 10 power supply fluo illum 11 control unit filter wheel ...

Page 12: ... the optical focus of the microscope at the object plane and to adjust the laser energy Figure 5 Laser Interface This is a prerequisite for precise laser micromanip ulations with highest precision Furthermore the laser position stays stable which allows to routinely work with the system without time consuming search for the laser beam focus LED color green yellow red indication status ready busy e...

Page 13: ...aser to the microscope The laser beam is guided into the microscope and deflected into the objective Figure 6 Microscope adapter The especially designed beam splitter see Figure 7 Pos 2 allows to connect the fluorescence illu mination lamp and the filter wheel to the system and simultaneously use fluorescence illumination with laser application Notice The correct laser focus setting is done by the...

Page 14: ...he microscope The filter revolver has to be in the position in which the beam splitter reflects the laserbeam This position is labeled with Laser In case of installed fluorescence attachment option it is possible to use also the installed beam splitters of the fluorescence filtersets only in case of original used P A L M beam splitters The two diaphragms in the epifluorescence channel must be open...

Page 15: ... integrated to speed up routine work In PALM RoboStage there is a holding frame into which the specimen slides and or Petri dishes can be clamped Both transversal sides of the object slide are held by a snap on clip One longitudinal side of the slide entirely rests on the holding frame This reduces the risk that extremely thin slides will bend by the adhesion force of the immersion oil 1 1 filter ...

Page 16: ...e arm moves back to the last position it was moved to If the arm has been moved from the reference position with the joystick then this position is accepted as the last targeted position If the button is pressed for a while then the cur rent position is saved as the new reference posi tion The LED will extinguish briefly as a confirmation signal Right button If the button is pressed briefly this w...

Page 17: ... image handling software for easy image selection and editing as well as archiving With the integrated network card you have the possibility to connect your computer with your local network If you want to connect it please contact your local area network administra tor Notice Depending of the Camera Adapter the com puter monitor only shows a section of the area visible in the ocular Caution The ca...

Page 18: ...lter block or slider in front of the objective is switched into the correct position called laser FL on adjustment failed that the FL shutter in the microscope is open FL appears in the display adjustment aid is in left position Notice If the red system LED of the control unit doesn t turn off automatically please witch off the PALM ControlUnit and after 10 seconds switch it on again Should the LE...

Page 19: ...ase see figure 14a The smallest avail able cutting size can only be achieved within the center of the laser focus using as little energy as possible The catapulting is effected by a laser beam wid ened to the appropriate diameter with its focus placed underneath the specimen to be catapulted please see figure 14b The laser beam is guided through the epifluo rescene channel of the microscope and is...

Page 20: ...tion the laser focus position has to be changed independent from the microscope focus The Laser Focus Correction is done motorized A high precision DC stepping motor moves the cor responding lens Focus setting is conveniently con trolled and regulated on the monitor screen by means of the of the PALM RoboSoftware The dis play allows relocating selected laser focus settings Laser energy and focus i...

Page 21: ...ic tool spanning at least the monitor screen start automatic cutting with the Cut function reduce the laser energy with the Page down key at the keyboard or with the slider for laser energy at the monitor screen until only a faint cut can be seen move the laser focus up and down with the keys home and end at the keyboard or the corresponding slider for laser focus at the mon itor screen to locate ...

Page 22: ...e 7 1 1 Simultaneous Fluorescence Observation The beam splitter in the fluorescence slide switch directly in front of the objective in the reflector slide or in the fluorescence cube is custom made The beam splitter must have a special coating suit able for the simultaneous reflection of the laser light and the fluorescence excitation wavelength as well as for the transmission of the emitted fluo ...

Page 23: ...tion of adjusting aid for the fluorescence lamp see section 5 5 page 14 left position o k right position switch to left check shutter for FL see section 5 5 page 14 shutter open FL on display o k shutter closed open shutter check position of filter revolver filter revolver on position laser o k any other position except you have fluorescence equipment with P A L M simultaneous cutting switch to la...

Page 24: ...ion between camera and computer S VHS plug must be plugged in framegrabber board check position of switching mirror in microscope check alignment of camera the camera must be adjusted parallel to the stage movement test this by moving the stage with the arrow keys in a slow speed g check position of laser marker Calibrate new as described in the User s Manual make sure that you are using the setti...

Page 25: ... be impaired Dirt on surfaces of lenses or filters burnt in by laser light can not be removed any more Slightly moisten a Kleenex tissue with pure alcohol 100 or spirit and wipe the dirty parts very carefully Clean dry objectives and oculars smoothly with a cotton bud or a tooth pick padded with cotton wool Only use surgical cotton from the phar macy Remove cotton fluffs and similar by blowing wit...

Page 26: ...0 Hz Frequency fluctuations 2 Hz Wavelength 337 nm Pulse energy 270 µJ Pulse duration 3 nsec Pulse frequency 1 30 pulses per second Beam divergence 0 3 mrad Laser control Laser control can be changed by impulse packages external by earth free foot stepper or via RS232 interface automatic permanent impulses Impulse width 0 1 ms Pulse frequency of laser control Adjustable via computer ...

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