Envirochek
®
and Envirochek HV
Sampling Capsules
• Designed for the concentration and recovery of
Cryptosporidium sp.
oocysts
and
Giardia sp.
cysts.
• Envirochek HV capsule optimized for sampling high volumes of source or
treated water.
Ordering Information
Part Number Description
Pkg
12098
Envirochek HV sampling capsule, bulk pack,
25/cs
individually
bagged
12099
Envirochek HV sampling capsule
1/pkg
12107
Envirochek sampling capsule, bulk pack,
25/cs
individually
bagged
12110
Envirochek sampling capsule
1/pkg
Specifications
Materials of Construction
Filter Media
12107, 12110: Supor
®
(polyethersulfone) membrane
12098, 12099: Polyester
Housing: Polycarbonate
End Caps: Vinyl
Filter Support Material: Polypropylene
Pore Size
1.0
μ
m
Effective Filtration Area
1300 cm
2
Dimensions
Length: 21 cm (8.5 in.)
Diameter: 6 cm (2.4 in.)
Inlet/Outlet Connections
12.7 mm (1/2 in.) straight hose barb
Maximum Operation Pressure
6.9 bar (690 kPa, 100 psi)
Maximum Differential Pressure
12107, 12110: 2.1 bar (210 kPa, 30 psid) at 21 ºC (70 ºF)
12098, 12099: 4.1 bar (410 kPa, 60 psid) at 21 ºC (70 ºF)
Sterilization
Provided non-sterile
Instructions for Use
This method does not address all of the safety concerns that are associated with
pathogen concentration and testing. It is the responsibility of the user of this device
and method to determine and employ all applicable safety measures to ensure the
safety of themselves and others. Check for regulatory limitations that may apply
prior to using this device.
Proper assembly and flow direction is critical to the filter’s performance. Improper
assembly can result in decreased throughput, lower sediment recoveries and, in
extreme cases, damage to the pleated membrane within the device.
Instructions for Use
(continued)
1.0
Materials
1.1
Envirochek or Envirochek HV sampling capsule, one capsule per
sample. The capsules are not reusable.
1.2
Hose-type tubing, 12.7 mm (0.5 in.) I.D.
1.3
Clamps
1.4
Water meter (flow totalizer)
1.5
Flow control valve or flow rate meter with valve
1.6
Pump, electric or gasoline powered (if needed)
1.7
Laboratory Shaker (Pall Life Sciences PN 4821A, 115V; 4822A, 230V)
1.8
Ice chest or cooler with cold packs
1.9
250 mL conical centrifuge tubes
1.10
50 mL polypropylene tubes
1.11
Elution Buffer
1.11.1 10 mL Laureth-12 solution (see below)
1.11.2 10 mL 1 M Tris, pH 7.4 (see below)
1.11.3
0
2 mL 0.5 M Ethylenediamine Tetraacetic Acid (EDTA),
2 Na, pH 8.0 (see below)
1.11.4
150
μ
L Antifoam A
1.11.5
5% w/v of Sodium Hexamethaphosphate (NaHMP) solution
(see below)*
1.11.6
Preparation of Laureth-12 Solution
Weigh 10 g of Laureth-12 (PN 4820) and dissolve in 90 mL
of reagent water using a microwave or hot plate. Dispense in
10 mL aliquots into sterile vials and store at room temperature
for up to 2 months or in a freezer for up to a year.
1.11.7
Preparation of 1 M Tris, pH 7.4
Dissolve 121.1 g Tris in 700 mL reagent water and adjust
pH to 7.4 using 1 N HCl or NaOH. Dilute to a final volume of
1000 mL with reagent water and adjust the final pH. Filter-
sterilize through a 0.2
μ
m membrane (PN 4632) into a sterile
plastic container and store at room temperature.
1.11.8
Preparation of 0.5 M EDTA, 2 Na, pH 8.0
Dissolve 186.1 g EDTA, sodiumdihydrate in 800 mL reagent
water and adjust to pH 8.0 using 6 N HCl or NaOH. Dilute to
a final volume of 1000 mL with reagent water and adjust the
final pH using 1 N HCl or NaOH.
1.11.9
Preparation of 5% w/v Sodium Hexametaphosphate Solution
Dissolve 50 g of sodium hexametaphosphate (NaPO
3
)
n
in
600 mL of reagent water. Dilute to final volume of 1000 mL
using reagent water. Store in a plastic or glass container at
room temperature for up to 3 months. Discard when expiration
date is reached or sooner if microbial growth is apparent.
1.11.10
Preparation of Buffer Solution
• Add the contents of a 10 mL vial of Laureth-12 solution to a
1000 mL graduated cylinder. Rinse the vial several times to
ensure the complete transfer of detergent.
•
Add 10 mL Tris solution, pH 7.4; 2 mL EDTA solution, pH 8.0;
and 150
μ
L Antifoam A to the graduated cylinder.
•
Bring solution to a final volume of 1000 mL with reagent
water. The buffer will have an opaque appearance.
Instructions for Use
(continued)
2.0 Sample
Collection
2.1
Retain the vinyl caps provided with the sampling capsule for sample
shipping and the elution process.
2.2
The exact installation is dependent on whether or not the water is from
a pressurized water source. If the source is not pressurized, a pump is
required. If a pump is required, the system will appear as in Figure 1a.
If a pump is not required, the system will appear as in Figure 1b.
Figure 1a: Sampling system with pump
Figure 1b: Sampling system without pump
2.3
Before connecting the sampling system to the pressurized system
or sample source, turn the pressurized system or pump on, allowing
water to purge residual debris from the water lines for 2-3 minutes, or
until the turbidity becomes uniform.
2.4
Turn off the pressurized source or pump and connect the sampling
system without the sampling capsule.
2.5
Turn on the pressurized source or pump and adjust the flow rate as
recommended by the current approved method for your region.
2.6
Allow approximately 2-10 L source or reagent water to flush the
system.
2.7
Install the sampling capsule in line, securing the inlet and outlet ends
with the appropriate fittings/clamps.
2.8
With a water-resistant marking pen, record the sampling location,
name of the person performing the sampling, date, meter reading, and
turbidity of the sample on the label provided on the filter housing.
2.9 Initiate water flow.
2.10
Vent the residual air in the capsule using the vent valve by turning it in
a counter clockwise direction. When the filter housing is full of water,
quickly close the vent valve.
2.11
After you have collected your sample through the sampling capsule,
shut off your water source. Allow the pressure to decay until flow stops.
2.12
Record the stop time and final meter reading in the appropriate spaces
on the label on the filter.
2.13
Disconnect the inlet end of the sampling capsule, making sure not to
spill any of the water remaining in the capsule. This water is part of
your sample.
2.14
Seal the inlet of the capsule with the vinyl end cap that was saved from
step 2.1.
2.15
Loosen the outlet end and allow water to drain as much as possible.
Water drainage from the capsule through the outlet is acceptable since
the sample has passed through the membrane.
2.16
Seal the outlet of the capsule with the vinyl end cap that was saved
from step 2.1.
2.17
Transport sampling capsules to testing facility according to
temperature and hold time requirements listed in current approved
method for your region.
Envirochek
Sampling
Capsule
Flow Totalizer
(meter)
Flow
Restrictor
Waste
Pump
Water
Source
Pressure
Regulator
Envirochek
Sampling
Capsule
Flow Totalizer
(meter)
Flow
Restrictor
Waste
Open/Close
Valve
Pressurized
Water
Source
*Note:
Use of NaHMP is accepted by EPA Method 1623.1 for the improved
recovery of cysts and oocysts in certain water matrices. If you elect to use this
step, this must be performed on all OPR’s and the same lot of elution buffer.
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