Instructions for Use
(continued)
3.0 Elution
Method
3.1
Assemble the Laboratory Shaker with the arms at full extension and
the clamps aligned in a vertical position so the capsules will be aligned
horizontally, as demonstrated in Figure 2.
Figure 2: Proper assembly of Laboratory Shaker
3.2a
*Dispersant Addition
3.2a.1
Record the elution date and time on the bench sheet. Using
a ring stand or other means, clamp each capsule in a vertical
position with the inlet end up.
Note:
Dispersant Addition cannot be performed on a sampling capsule
through which water can no longer be filtered (i.e. clogged). Record on
the bench sheet that the sampling capsule is clogged, and proceed to
Elution Method.
3.2a.2
Remove the inlet cap, pour NaHMP solution through the inlet
opening, and allow the liquid level to stabilize. Sufficient NaHMP
solution must be added to cover the pleated white membrane
with NaHMP solution or NaHMP solution may be measured to
125 mL. Replace the inlet cap.
3.2a.3
Securely clamp the capsule in one of the clamps on the
laboratory shaker with the bleed valve positioned at the top on a
vertical axis (in the 12 o’clock position). Turn on the shaker and
set the speed to maximum (700-900 rpm or per manufacturer’s
instructions). Agitate the capsule for approximately 5 minutes.
Time the agitation using a lab timer, rather than the timer on the
shaker to ensure accurate time measurement.
3.2a.4
Remove the filter from the shaker, remove the outlet cap, and
attach the capsule filter outlet to tubing, upstream of a pump.
Holding the filter upright, remove the inlet cap, being careful not
to pour any liquid from the inlet, turn on the pump and allow
pump to pull all the NaHMP through the filter, turn off pump. Do
not allow the filter pleats to collapse during the pumping process.
3.2a.5
Fill the capsule with reagent water, pinching the outlet hose
if necessary, to cover the white pleated membrane and the
plastic above the membrane; allow the liquid level to stabilize.
Sufficient reagent water must be added to cover the pleated
white membrane. Turn on the pump and allow pump to pull all
the water through the filter. Turn off the pump.
3.2a.6
Replace the inlet cap. Disconnect the outlet tubing from the
filter, and replace the outlet cap. Proceed directly to elution
within the same working day.
3.2
Prepare sufficient elution buffer so all the samples to be eluted that day
can be eluted with the same stock elution buffer solution. (see 1.11)
3.3
Designate a 250 mL conical centrifuge tube for each sample and label
with the sample number.
3.4
If the upper chamber of the capsule has water remaining in it, pull the
remaining fluid through the filter before eluting the filter.
3.5
Hold the capsule in a vertical position with the inlet end up by using a
ring stand or other means. Remove the vinyl end cap.
If eluting standard volumes of source water, proceed to step 3.6, If
eluting high volumes of drinking water continue with step 3.5.1:
3.5.1
Fill the sampling capsule with a 5% weight by volume of sodium
hexametaphosphate in reagent water.
3.5.2
Replace the vinyl end cap on the inlet and securely clamp the
sampling capsule on the laboratory shaker.
3.5.3
Turn on the shaker, set the speed at approximately 600 rpm,
and agitate for 5 minutes.
3.5.4
Turn off the shaker, remove the sampling capsule, hold upright
and remove the vinyl end cap from the outlet.
3.5.5
Loosen the vent valve and allow the sodium hexametaphosphate
solution to drain through the capsule membrane and out the outlet.
3.5.6
Tighten the vent valve and replace the vinyl end cap on the outlet
of the sampling capsule.
Instructions for Use
(continued)
3.5.7
Hold the capsule in the vertical position with the inlet end up by
using a ring stand or other means. Remove the vinyl end cap
from the inlet.
3.5.8 Repeat steps 3.5.1 through 3.5.7 using reagent water.
3.6
Add elution buffer to the inlet end of the capsule and allow liquid level
to stabilize. Sufficient elution buffer should be added to the capsule to
ensure the covering of the pleated white filter module by approximately
13 mm (0.5 in.) (see Figure 3). Replace the vinyl end cap to the inlet
end of the capsule.
Figure 3: Elution buffer level
3.7
Securely clamp the capsule on the Laboratory Shaker so the vent valve
is facing toward you. Make sure the vent valve is in the 12 o’clock
position (see Figure 4).
3.8
Turn on the shaker and set the speed at approximately 600 rpm and
agitate for 5 minutes.
3.9
Remove the sampling capsule from the shaker. Carefully remove the
inlet end cap and decant the contents of the capsule into a 250 mL
conical centrifuge tube.
3.10
Add new elution buffer to the inlet end of the capsule and allow liquid
level to stabilize. Sufficient elution buffer should be added to the
capsule to ensure the covering of the pleated white filter module by
approximately 13 mm (0.5 in.) (see Figure 3). Replace the vinyl end cap
to the inlet end of the capsule.
3.11
Again, securely clamp the capsule on the Laboratory Shaker so the vent
valve is facing toward you. This time make sure the vent valve is in the
4 o’clock position (see Figure 4).
3.12
Turn on the shaker and set the speed at approximately 600 rpm and
agitate for 5 minutes.
3.13
Turn off the shaker and loosen the clamp but
do not remove the
capsule and decant the elution buffer.
3.14
Rotate the capsule until the vent valve is in the 8 o’clock position
(see Figure 4), secure the capsule in place, and agitate at 600 rpm for
5 minutes.
3.15
As in step 3.9, remove the capsule from the shaker, carefully remove
the inlet end cap and decant the contents of the capsule into the same
250 mL conical centrifuge tube labeled for that sample.
Figure 4: Orientation of sampling capsules during elution
Fill to this level
with elution buffer.
1.0 m Sampling Capsule
PN. 12099
Pall Corporation
Vent Valve
Vent Valve
Vent Valve
First Cycle
Second Cycle
Third Cycle
12 o’clock position
4 o’clock position
8 o’clock position
International Of
Ä
ces
Pall Corporation has of
Ä
ces and plants throughout the world in; Argentina, Australia, Austria, Belgium, Brazil, Canada,
China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, New Zealand, Norway, Philippines,
Poland, Russia, Singapore, South Africa, Spain, Sweden, Switzerland, Taiwan, Thailand, United Kingdom, and
Vietnam. Distributors in all major industrial areas of the world. To locate the Pall of
Ä
ce or distributor nearest you, visit
www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be
subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
Visit us on the Web at www.pall.com/lab
E-mail us at [email protected]
Pall Life Sciences
25 Harbor Park Drive
Port Washington, NY 11050 USA
Instructions for Use
(continued)
4.0 Concentration: Adjustment of Pellet Volume
4.1
Spin the sample at 1500 x G for 15 minutes. Allow the centrifuge to
coast to a stop. DO NOT USE THE BRAKE!
4.2
Record the volume of the pellet with date and time the concentration
was completed. Use a Pasteur pipette to carefully remove the
supernatant to 5 mL above the pellet. Refer to current approved
method for instructions on pellet analysis.
5.0 Detection
5.1
The sample is now ready for the detection stage of the process. Refer
to the current approved detection method for your region.
WARNING
Employment of the products in applications not specified, or failure to follow
all instructions contained in this product information insert, may result in
improper functioning of the product, personal injury, or damage to property
or the product. See Statement of Warranty in our most recent catalog.
This method is in accordance with EPA Method 1622:
Cryptosporidium
in
Water by Filtration; & EPA Method 1623 & 1623.1:
Cryptosporidium
and
Giardia
in Water by Filtration; however, consult your local regulations to
ensure compliance.
Go to www.pall.com/lab for these instructions translated into multiple languages.
© 2014, Pall Corporation. Pall,
, Envirochek, and Supor are trademarks of Pall Corporation. ® indicates a
trademark registered in the USA.
09/14
PN 87997D
1401_87997D.indd 2
1401_87997D.indd 2
8/27/14 10:32 PM
8/27/14 10:32 PM
