OROBOROS O2k-Core Manual
Mitochondrial Physiology Network 19.18(B04):17-22 (2016)
Version B04: 2016-08-08 DatLab 7
2014-2016 OROBOROS
B
:
http://wiki.oroboros.at/index.php/MiPNet19.18B_POS-Service
OROBOROS INSTRUMENTS
high-resolution respirometry
Service of the
polarographic
oxygen sensor
OroboPOS
Gnaiger E
OROBOROS INSTRUMENTS
Schöpfstr 18, A-6020 Innsbruck, Austria
1. OroboPOS-Service Kit ............................... 18
2. Cleaning ................................................. 18
3. Membrane mounting ................................ 20
4. Electrical cable connection ........................ 21
5. Storage of the OroboPOS .......................... 21
6. Replacement of the OroboPOS head ........... 22
7. References .............................................. 22
Supplement: Videosupport
Summary
Service of the polarographic oxygen sensors (
POS
) is the basis of signal stability, low noise and high time resolution.
Performance specifications of the OROBOROS O2k can be met only with
oxygen sensors which are maintained in a well monitored functional state.
POS service may not be required
for several months of operation.
POS service is necessary if (1) a
new sensor is prepared, (2) the
signal at air calibration is not stable
over time, (3) signal noise is high,
(4) the time response is prolonged
and biphasic (time constant >10 s),
(5) the oxygen signal at zero calibration does not decline rapidly to 0% to
max. 5% of the signal at air saturation. For each sensor, the
frequency of POS service is optimized on the basis of long-term
calibration records for
quality control
(
O2-calibration.xlsx
).
B
