MiPNet19.18
B: OroboPOS service
21
OROBOROS INSTRUMENTS
high-resolution respirometry
B
•
Mounting a membrane onto the OroboPOS
, using
the OroboPOS-Mounting Tool which consists of two
parts, the membrane guide and the membrane ring
holder.
No gas bubbles
should be trapped
in the electrolyte
reservoir
after
membrane
application.
No
folds should be
visible
in
the
membrane in the
central
area.
Inspect
the
electrolyte
reservoir under a
binocular. Small
folds
in
the
membrane near
the
outer
circumference
have
no
influence, but large folds should be avoided. Wash excess electrolyte off the
POS and POS connector. Apply a wet
4.
Electrical cable connection
Insert the male plug of the cable of the
next to the control light of the stirrer (O2k-Main
Unit). The red dot on the male plug has to face
accurately upwards when inserting the plug.
Avoid bending and torsions of the cable.
» MiPNet19.01 for O2k Series B to C.
After sensor service and membrane mounting, the POS needs some
time in operation to stabilize while the O2k is switched on. Such
stabilization may require several hours. For this purpose the O2k may be
switched on and run overnight.
5.
Storage of the OroboPOS
5.1. Short-term storage in the O2k
For short periods of days or several weeks, the POS is maintained in the
O2k chamber. The chamber is washed with distilled water and completely
filled with 70% ethanol for chemical sterilization. The stopper is inserted
loosely without pushing it down beyond the point where the sealing ring is
inserted into the glass chamber. This ensures a longer life time of the
sealing rings. The receptacle of the stopper is completely filled with
ethanol from the top, and is sealed with a black cover slip to avoid
