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                                                                                                      A16.1065 

position,  seen  from  the  front  of  the 
microscope:  If  you  select  the  bright-field 
position,  the  filter  cube  is  removed  from  the 
optical  path.  The  three  bright-field  positions 
have the same function. 
Rotate  the  turret  so  that  the  fluorescence 
positions  indicated  by  the  arrows  on  the  left 
and right are the same (all points to “1” in the 
right  figure),  indicating  that  the  filter  cube 
has been switched into the optical path.   

 

One-to-one corresponding relationship 
between filter cube sign, fluorescent 
LED light signs and filter cube should 
be paid attention to, when using. (see 
section 4.3.2 for the position of 
fluorescent LED lights)

 

 

3.9.4

 Fluorescence Filter Cube

 

A filter cube consists of three types of optical 
components: an excitation filter (BP filter), a 
barrier filter (BA filter), and a dichroic mirror 
(DM). 
Referring  to  the  following  items  as  a  guide, 
select  a  desired  combination  of  filter  cubes 
according  to  the  characteristics  of  the 
specimen and fluorophore. 
•  You  can  select  a  combination  of  an 
excitation  filter  and  a  barrier  filter  even  if 
you are using the same excitation method. 
•  Excitation  filters,  barrier  filters,  and 
dichroic mirrors can be purchased separately. 
• Excitation filters are exposed to strong light 
during  operation  and  will  deteriorate  over 
time. Replace the filter as appropriate. 

 

3.9.5

 Fluorescent LED Unit 

The 410-FL allows you to mount up to three 
different fluorescence LED units as the light 
source for episcopic fluorescence 
microscopy. 
When the fluorescence cube turret is rotated, 
the fluorescence LED unit is switched 
automatically. Therefore, mount the 
fluorescence LED unit at the same address as 
the filter cube to be used. 

 

Dichroic mirror 

SIGN 

Summary of Contents for A14.1065

Page 1: ...B G U Instruction Manual This instruction manual is intended for users of the Inverted Series Biological Microscope A16 1065 To ensure safety use perform the best performance and to familiarize yours...

Page 2: ...Specimen Placement and Stage Knob Adjustment 10 3 3 1 Mechanical Stage 10 3 3 2 Pallet 11 3 3 3 Auxiliary Stage 11 3 4 Adjusting the Interpupillary Distance 11 3 5 Focusing on the Specimens 12 3 5 1...

Page 3: ...5 Installing the Eyepiece 20 4 2 6 Installing the Camera 20 4 3 Assembly Episcopic Illumination Microscopy 21 4 3 1 Installing the Filter Cube 21 4 3 2 Installing the Fluorescence LED Unit 21 4 3 3 R...

Page 4: ...event lens glass to stick to fingerprint perspiration other factors influence observation prevent lens and other attachment drop damaged 2 Avoid placing the microscope in place with direct sunlight hi...

Page 5: ...e rear of the main body Do not hold any other parts such as the upper part of the illumination pillar focus knobs eyepiece tube or stage when carrying the microscope Doing so might result in dropping...

Page 6: ...use Cover up must wait for the lamp room to cool down 9 The microscope usage environmental requirements a For indoor use b The environment temperature range 10 35 c Maximum relative humidity 80 at 31...

Page 7: ...tive 4 Phase contrast objective 10 Phase contrast objective 20 Phase contrast objective 40 Infinity plane semi apochromatic objective 10 Infinity plane semi apochromatic objective 20 Infinity plane se...

Page 8: ...Magnification 4 10 20 40 10 20 40 numerical aperture N A 0 10 0 25 0 40 0 60 0 30 0 45 0 60 Work distance mm 30 10 2 12 2 2 7 4 8 3 6 Cover glass thickness 0 17 0 17 0 17 0 17 1 2 1 2 1 2 Conjugate d...

Page 9: ...A16 1065 1 2 Components The microscope A16 1065 components following diagrams A16 1065 Viewing head Camera mount LCD Mainframe Nosepiece Stage Condenser Objective Contrast slider Moving stage Eyepiec...

Page 10: ...nents following diagrams A16 1065 FL Viewing head Camera mount LCD Mainframe Fluorescence illumination Stage Condenser Filter Cube turret Fluorescent baffle Eyepiece Nosepiece Objective Moving stage U...

Page 11: ...adjustment knob 7 Condenser brightness 2 Condenser rotating knob 8 Fine focus knob 3 Condenser adjustment knob 9 Coarse focus knob 4 aperture diaphragm adjusting knob 10 Power indicator 5 Nosepiece 1...

Page 12: ...9 A16 1065 1 Bracket horizontal movement knob 2 Bracket vertical movement knob 3 Fluorescence cube turret A16 1063 3 1 2...

Page 13: ...careful when changing the objective The objective may collide with the specimen because of the short working distance of specimen observation 3 3 1 Movving stage The Movving stage is mounted and used...

Page 14: ...er 3 3 3 Auxiliary stage Connect the auxiliary stage to the left and right of the stage to expand the stage width After connecting the auxiliary stage the stage width is 300 mm 3 4Adjusting the Interp...

Page 15: ...or about 4 mm trip to limit The traveling distance of the objective for each knob is as follows Rotation of knob Distance traveled by objective Fine focus knob 1 rotation 0 2 mm Coarse focus knob 1 ro...

Page 16: ...result in an appropriate contrast and a favorable image The iris aperture adjusting handle is marked with the aperture of the diaphragm 3 7 Nosepiece Turn the nosepiece until it clicks in place stays...

Page 17: ...aphragm image with the phase ring image in the objective Decentering the phase ring and the PH annular diaphragm Basically the phase ring and the PH annular diaphragm should be adjusted so that they a...

Page 18: ...and switch the filter cube by rotating the filter cube switching unit 3 9 3 Filter cube switching unit Rotating the filter cube switching unit switches the filter cube When the filter cube is switched...

Page 19: ...on filter BP filter a barrier filter BA filter and a dichroic mirror DM Referring to the following items as a guide select a desired combination of filter cubes according to the characteristics of the...

Page 20: ...minute scale figures flash then click the button hour scale figures become flashing When the time figures flash three times it means setting successful Double click locking brightness or unlocking Whe...

Page 21: ...h component is shown in the following figure and the digital indicate the installation step Save the supplied hexagonal spanner You need it when you change parts CAUTION Before installation make sure...

Page 22: ...contrast slider horizontally into the condenser socket and notice the adjusting screw side of the phase contrast slider is facing the operator side Push into the process when you hear the click sound...

Page 23: ...itching the objective rotate the objective until it clicks to ensure that the desired objective enters the center of the optical path 4 2 5 Installing the Eyepieces Insert the eyepiece into the eyepie...

Page 24: ...scope and remove the cover 2 Mount the LED unit corresponding to the desired wavelength at the position that matches the address of the filter cube The back cover of the LED unit is marked with positi...

Page 25: ...ff immersion oil from objectives Do not use petroleum benzene to wipe the prism surface of the eyepiece tube or filters 5 1 1 Cleaning the Lenses Keep the lenses free of dust fingerprints and other di...

Page 26: ...disposal If a sample comes into contact with this product check the sample is hazardous or not If the sample is hazardous follow the standard procedure of your laboratory 5 2 Storage Store this produ...

Page 27: ...ced reversely Turn it over A lens the objective condenser eyepiece or collector is dirty Clean it The aperture diaphragm is too large Appropriate down Dirt or dust on the eyepiece Clean it The apertur...

Page 28: ...llary Distance does not adjust Adjust the Interpupillary Distance correctly the illumination is not appropriate Adjust illumination switch 6 3 Electrical System TROUBLE CAUSE SOLUTION The bulb cannot...

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