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A16.1065
3.8 Operation the Phase Contrast Microscope
Phase contrast microscopy requires phase contrast (PH) objectives and PH sliders
.
If PH Slider is used, it is possible to center the annular diaphragm. Turn the centering
screw of the PH slider to merge the annular diaphragm image with the phase ring
image in the objective.
Decentering the phase ring and the PH annular diaphragm
Basically, the phase ring and the PH annular diaphragm should be adjusted so
that
they
are concentric
.
However, slightly decentering them will produce a shadowing effect,
resulting in a three-dimensional image.
Annular diaphragm
Centering hold
(on the side)
Hollow position
Centering hold
(on the side)
Hexagonal
screwdriver
Centering screws
Hexagonal
screwdriver
