24
8. Dark Field Observation
8.1 Bright and Dark Filed
Components (Fig.36)
The halo
①
used in the dark filed
observation is dark filed illumination
board, while the opening
②
is used for
bright filed
8.2 Installation and Use
(
Fig.37
)
1. Before installing the bright and dark filed
component, pull out the phase contrast
slider in the reverse order of
7.2.1
.
2. Keep the lettering face of bright and dark
filed component forward, then insert it
into
the
corresponding
hole
of
condenser. After inserting, tighten the
screws
③
on the left and right sides
★
When observation in the dark filed, a
drop of cedar oil between the
condenser and specimen should be
filled. Otherwise the light will be
completely reflected on the condenser
and cannot reach the inspected
object,
so
that
the
dark
filed
illumination is not obtained.
★
Before observing specimens in dark
field, the center adjustment and focus
of the condenser must be carried out
to make the focus consistent with the
inspected object.
Fig.36
Fig.37
①
②
③