22
7. Phase Contrast Observation
7.1 Components Name
7.2 Installation And Use
Fig.33
①
②
③
④
7.1.1 Phase Contrast Objective
The optional objective magnification of
the phase contrast is :10×,20×,40×,100×.
If you want to know how to mount the
phase contrast objective, please see
2.2.2
. You ought to mount it on the
nosepiece.
7.1.2 Phase Contrast Slider
（
Fig.33
）
The diaphragm has been centered
beforehand, so it needn’t to adjust in the
use process. If the diaphragm is not in the
center, it can be adjusted by the
centering screw
①
.
The 10×/20×/40×diaphragm
②
matches the
10×/20×/40×phase contrast objective, and
100x diaphragm matches 100×phase
contrast objective, while the opening
③
is
used for bright field .
7.2.1 Installing the Phase Contrast
Slider
（
Fig.34
）
◎
The phase contrast slider has been installed
before leaving factory, so the user needn’t
to install it by self.
1. Keep the lettering face of phase contrast
slider forward, then insert it into the
corresponding hole of condenser from left
to right. After inserting, tighten the screws
④
on the left and right sides
2. Every diaphragm or opening has its own
located position, so you need to move
them until you heard the “clicked” to
ensure the diaphragm or the opening
reach the center of the light path.
3.
The aperture iris diaphragm must be
opened to maximum for phase contrast
observation.
★
The screws
④
on the left and right
sides have positioning effect to prevent
the phase contrast slider from being
pulled out.
Fig.34
