Optika B-1000 POL, Instruction Manual

Page: 10 / 80

Page 10
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Fully insert the phase contrast condenser into the holder, pushing it until it is well inserted:
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Rotate the condenser annuli disc to the “BF” position (brightfield).
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Completely open the aperture diaphragm:
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Turn on the light and put a sample slide on the stage (an opaque specimen will make alignment easier).
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Using the 10x objective, center your sample (moving the stage) and focus using the coarse and fine focus
knobs.
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Rotate the condenser height adjustment knobs until you obtain a uniform white illumination on the sample.
Important: the optimal position corresponds to the top lens of the condenser 1-2mm below the bottom side
of the slide.
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Rotate the condenser annuli disc to the “10” position.
Condenser must fully enter into its guide