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3.5 Focusing
1) With the 10x objective in position, raise the mechanical stage using the coarse
focus knob until the specimen is close to the objective
2) Turn the coarse focus knob until the specimen is in
focus. Use the fine focus knob to obtain a sharp
image. You may now switch to another magnification
objective.
3) To get a good focused image, you may need to
combine the focus knob adjustment and interpupillary
distance adjustment, along with eyepiece diopter
adjustment stated in
3.3
and
3.4
.
Tips:
T
o prevent your specimen slide from making contact with an objective, turn the
100X objective in position and adjust the focusing rack stop screw (
Fig. 5
) so that
the 100X objective will not contact the specimen while the stage is adjusted to its
highest position. Give the stage a tiny extra moving space to ensure the objective
can be focused every time.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in
Fig. 6
(a)
.
2)
Place a drop of immersion oil on the slide cover as shown in
Fig. 6
(b)
.
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the camera cleaning kit to clean the 100X
objective lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens
and the specimen.
Caution (important):
When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
When you apply the immersion oil with the 100X objective, do not let the
immersion oil to contact with the dry objective lenses (especially the 40x). If the
immersion oil is on the dry objectives lens, please use the camera cleaning kit to
clean the objectives lens in time. The oil will damage the dry objective lenses.
Fig. 5
Focusing Rack Stop Screw
(a)
Fig. 6
(b)
(a)
