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Olympus IX81-ZDC2, Instructions Manual, Page: 9 / 16

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Olympus IX81-ZDC2 Instructions Manual Download Page 9

6

IX81-ZDC2

7

} The IX81-ZDC2 can be combined with the same modules as the IX81 microscope systems, but certain modules are 

subject to restrictions with regard to the combination with the stages, thermal incubation boxes (IBMU/IBML) and interme- 
diate attachments.

  In addition, troubles such as “obscuring” or “insufficient peripheral brightness” may occur depending on combination of 

modules (see “6. Restrictions on combined modules” on page 5).

MODULE NOMENCLATURE

Transmitted Light Lamp 

Housing

Condenser

 · IX2-LWUCDA2

 · IX2-LWUCD

 · IX-ULWCD

 · IX2-DICD

 · IX2-MLWCD

 · U-UCD8 

   (Used together

    with IX-ADUCD)

Eyepieces

 · WHN10X

 · WHN10X-H

Stage

 · IX2-SFR

 · IX2-GS

 · IX2-SP 

   (Can be used

    together with

    IX-MVR)

Observation Tube

 · U-BI90CT

 · U-BI90

 · U-TBI90

 · U-TR30H-2 

   (Used together

    with IX-ATU)

Transmitted Illumination 

Column

Fluorescence Mirror Unit 

Cassette

 · IX2-RFACA

 · IX2-RFAC

Reflected Fluorescence 

Illuminator
 · IX2-RFA

 · IX2-RFAL

 · IX2-RFAW

 · IX2-RFAEVA-2

 · IX2-ARCEVA

IR slider

Microscope Frame

 · IX81F-ZDC2
 · IX81S1F-ZDC2

Reflected Light

Lamp Housing

Auto Focus Sensor Unit
IX2-ZDC2

Do not remove this sensor unit 

since it is a part of the IX81-ZDC2 

system  and  assembled  by  the 

manufacturer.  Do  not  apply  ex-

cessive force to the auto focus 

sensor  unit  and  do  not  place 

anything on it.

Reflected Light 

Filter Wheel

Shutter

High-Intensity

Power Supply 

Unit

Camera 

adapter

CCD camera

1

Summary of Contents for IX81-ZDC2

Page 1: ...oscope Model IX81 ZDC2 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference A X 7 9 0 6 ...

Page 2: ... likely to cause harmful interference in which case the user will be required to correct the interference at his own expense FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Compliance This device complies with the requirements of both directive 2004 108 EC concerning electromagnetic compati...

Page 3: ...6 7 8 11 12 IMPORTANT Be sure to read this section for safe use of the equipment 1 MODULE NOMENCLATURE 2 NOMENCLATURE 3 OPERATION 4 SPECIFICATIONS 5 TROUBLESHOOTING GUIDE 1 5 CONTENTS IX81 ZDC2 ...

Page 4: ... equipment damage 7 To prevent the microscope system from falling down do not install modules which cause the microscope system height to exceed 1 meter 8 Do not use an objective other than those specified to be applicable see page 4 Safety Symbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Ex...

Page 5: ...for example electrical equipment that is being switched on or off which could cause ignition of a fire Also remember to always use it only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble or modify any...

Page 6: ...multi point time lapse observation 2 Continuous focus mode In this mode the laser beam is focused continuously on the top surface of the cover glass of the glass bottom dish at the same time as the target cell During this the focus is corrected automatically even when the change in environmental temperature due to heat cause defocusing The range in which continuous focusing is possible is determin...

Page 7: ... 60X O 1 42 0 15 UPLSAPO 60X O UPLSAPO 100X O 1 35 1 40 0 15 0 13 UPLFLN 100X O2 UPLFLN 100X OI2 1 30 1 30 0 60 0 20 0 20 UPLSAPO 20X UPLSAPO 40X2 UPLSAPO 60X W 0 75 0 95 1 20 0 60 0 18 0 28 UPLFLN 20X UPLFLN 40X UPLFLN 60X 0 50 0 75 0 90 2 10 0 51 0 20 LUCPLFLN 20X LUCPLFLN 40X LUCPLFLN 60X 0 45 0 60 0 70 6 60 7 80 2 70 4 00 1 50 2 20 UAPO 40X OI3 340 1 35 0 65 0 1 Compatible with one shot focus ...

Page 8: ...sed the only TV adapter that can be used is the U TV1X The FN of the right side port is specified as 16 When a 10X or lower power objective a CCD camera are used the brightness in the peripheral area may be slightly insufficient When a 2X 4X or 10X objective is used with FN 22 the brightness in the peripheral area may be slightly insufficient This problem does not occur with FN 16 When a 10X objec...

Page 9: ...r with IX ADUCD Eyepieces WHN10X WHN10X H Stage IX2 SFR IX2 GS IX2 SP Can be used together with IX MVR Observation Tube U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU Transmitted Illumination Column Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Reflected Fluorescence Illuminator IX2 RFA IX2 RFAL IX2 RFAW IX2 RFAEVA 2 IX2 ARCEVA IR slider Microscope Frame IX81F ZDC2 IX81S1F ZDC2...

Page 10: ...em Focus Handle Interface U IFFH CCD camera 2 Focus Handle U FH AF port switching lever Pulled out position OUT Pushed in position IN Wavelength 785 nm Beam divergence Objectives attached 0 to 1 49 NA Depend on objective lens 2Objectives removed 0 14 to 0 03 deg Depend on offset lens position Pulse duration 2 5 ms Pulse repetition rate 100 Hz Maximum power output 127 5 µW AF port switching unit PC...

Page 11: ...be obtained by judging the image resolution and contrast When a satisfactory image is not obtained after focusing rotate the correction collar to the left and right refocus each time and compare the images at both sides Then rotate the collar in the direction yielding a better image and rotate the collection collar to the left and right refocus each time and compare the images Repeat this cycle un...

Page 12: ...ded Make sure the oil is free of air bubbles a To check for air bubbles remove the eyepieces completely open the field iris diaphragm and aperture iris diaphragm and look at the objective exit pupil looking like a bright circle in the observation tube Any air bubbles can be seen in this way b To remove air bubbles slightly rock the revolving nosepiece manually to engage and disengage the oil immer...

Page 13: ...t mode Offset Setup of the amount of compensation between the cover glass top surface and target position AF zone AF search zone AF center width and range In continuous focus mode Confirm that the cover glass top surface is detected in the one shot mode before switching to the continuous focus mode For details refer to the instruction manual and Help of the application When using the MetaMorph app...

Page 14: ...servations Field number 22 AF focusing speed One shot focus mode only Approximately 1 to 5 sec from the focusing range Excluding the offset drive time Note The AF speed may slow down depending on the PC setup AF repeatability 20X and 40X objectives 0 1 μm with constant temperature 0 17 μm with less than 5 C temperature change 60X objective or 40X 60X 100X oil water immersion objectives 0 07 μm wit...

Page 15: ...sh is focused Set the AF zone so that its center is located on the interface between the culture fluid and glass bottom dish and the AF zone width is 10 µm 10 b Autofocusing takes long time or fails AF is set improperly Set up AF properly For the setting method refer to the instruction manual for the application software 10 Electrical noise is interfering with op eration Ground the IX2 UCB Be also...

Page 16: ...2 Printed in Japan on May 19 2010 M 003 01 ...

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