Olympus IX81-ZDC2 Instructions Manual Download Page 4

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1. The focus drift compensation function uses a laser diode (wavelength 785 nm) for autofocusing. It is designed to be safe 

by reducing the laser power (Class 1), it is still not recommended to view the laser light directly by removing the cover, etc.

  Never remove the warning and caution labels on the microscope.
  The semiconductor laser for autofocusing incorporated in this unit makes it designated as a product of the following 

class.

 

CLASS 1 LASER PRODUCT (IEC60825-1:1993+A1:1997+A2:2001)

2. Never attempt to remove the cover using a tool. There is a risk of exposure to the internal strong laser beam as well as 

other malfunction or failure.

  [Semiconductor laser, wavelength 785 nm, output (max.) 85 mW, equivalent to Class 3B]
3. Always use the power cord provided by Olympus. If the proper power cord is not used, product safety performance 

cannot be warranted.

4. Always ensure that the 

grounding terminal

 is properly grounded. If the equipment is not grounded, Olympus can no 

longer warrant the electrical safety performance of the equipment.

5. Distribute the connection cables at a distance from the lamp housing. If a connection cable contacts the lamp housing 

or its surroundings, the cable may be melted and cause n electric shock hazard.

6. Never insert metallic objects into the air vents of the microscope as this could result in electrical shock, personal injury 

and equipment damage.

7.  To prevent the microscope system from falling down, do not install modules which cause the microscope system height 

to exceed 1 meter.

8. Do not use an objective other than those specified to be applicable (see page 4).

  Safety Symbols

  The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment 

in the safest possible manner.

Symbol

Explanation

Before use, carefully read the instruction manual. Improper use could result in personal injury to 
the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

IMPORTANT

} The IX81-ZDC2 microscope features the capability of compensating for the focus drift that poses problems 

during long hours of time-lapse observation of live cells.

  The microscope has two focus drift compensation modes including the one-shot mode and continuous focus 

mode, which are to be selected according to the observed specimen.

} This manual pertains only to the information related to the IX81-ZDC2 (focus drift compensation) facility of the 

microscope. Please also refer to the instruction manuals for the microscope and associated modules.

} The IX81-ZDC2 can be controlled with the MetaMorph application software. For the compatible version of the 

software, consult the respective company.

} For the applicable modules, contact Olympus or check the latest catalogue for this product.

Safety Precautions

This product complies with 21 CFR 1040.10 and 1040.11 except for deviations 
pursuant to Laser Notice No.50, dated (June 24, 2007).

Summary of Contents for IX81-ZDC2

Page 1: ...oscope Model IX81 ZDC2 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference A X 7 9 0 6 ...

Page 2: ... likely to cause harmful interference in which case the user will be required to correct the interference at his own expense FCC WARNING Changes or modifications not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Compliance This device complies with the requirements of both directive 2004 108 EC concerning electromagnetic compati...

Page 3: ...6 7 8 11 12 IMPORTANT Be sure to read this section for safe use of the equipment 1 MODULE NOMENCLATURE 2 NOMENCLATURE 3 OPERATION 4 SPECIFICATIONS 5 TROUBLESHOOTING GUIDE 1 5 CONTENTS IX81 ZDC2 ...

Page 4: ... equipment damage 7 To prevent the microscope system from falling down do not install modules which cause the microscope system height to exceed 1 meter 8 Do not use an objective other than those specified to be applicable see page 4 Safety Symbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Ex...

Page 5: ...for example electrical equipment that is being switched on or off which could cause ignition of a fire Also remember to always use it only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble or modify any...

Page 6: ...multi point time lapse observation 2 Continuous focus mode In this mode the laser beam is focused continuously on the top surface of the cover glass of the glass bottom dish at the same time as the target cell During this the focus is corrected automatically even when the change in environmental temperature due to heat cause defocusing The range in which continuous focusing is possible is determin...

Page 7: ... 60X O 1 42 0 15 UPLSAPO 60X O UPLSAPO 100X O 1 35 1 40 0 15 0 13 UPLFLN 100X O2 UPLFLN 100X OI2 1 30 1 30 0 60 0 20 0 20 UPLSAPO 20X UPLSAPO 40X2 UPLSAPO 60X W 0 75 0 95 1 20 0 60 0 18 0 28 UPLFLN 20X UPLFLN 40X UPLFLN 60X 0 50 0 75 0 90 2 10 0 51 0 20 LUCPLFLN 20X LUCPLFLN 40X LUCPLFLN 60X 0 45 0 60 0 70 6 60 7 80 2 70 4 00 1 50 2 20 UAPO 40X OI3 340 1 35 0 65 0 1 Compatible with one shot focus ...

Page 8: ...sed the only TV adapter that can be used is the U TV1X The FN of the right side port is specified as 16 When a 10X or lower power objective a CCD camera are used the brightness in the peripheral area may be slightly insufficient When a 2X 4X or 10X objective is used with FN 22 the brightness in the peripheral area may be slightly insufficient This problem does not occur with FN 16 When a 10X objec...

Page 9: ...r with IX ADUCD Eyepieces WHN10X WHN10X H Stage IX2 SFR IX2 GS IX2 SP Can be used together with IX MVR Observation Tube U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU Transmitted Illumination Column Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Reflected Fluorescence Illuminator IX2 RFA IX2 RFAL IX2 RFAW IX2 RFAEVA 2 IX2 ARCEVA IR slider Microscope Frame IX81F ZDC2 IX81S1F ZDC2...

Page 10: ...em Focus Handle Interface U IFFH CCD camera 2 Focus Handle U FH AF port switching lever Pulled out position OUT Pushed in position IN Wavelength 785 nm Beam divergence Objectives attached 0 to 1 49 NA Depend on objective lens 2Objectives removed 0 14 to 0 03 deg Depend on offset lens position Pulse duration 2 5 ms Pulse repetition rate 100 Hz Maximum power output 127 5 µW AF port switching unit PC...

Page 11: ...be obtained by judging the image resolution and contrast When a satisfactory image is not obtained after focusing rotate the correction collar to the left and right refocus each time and compare the images at both sides Then rotate the collar in the direction yielding a better image and rotate the collection collar to the left and right refocus each time and compare the images Repeat this cycle un...

Page 12: ...ded Make sure the oil is free of air bubbles a To check for air bubbles remove the eyepieces completely open the field iris diaphragm and aperture iris diaphragm and look at the objective exit pupil looking like a bright circle in the observation tube Any air bubbles can be seen in this way b To remove air bubbles slightly rock the revolving nosepiece manually to engage and disengage the oil immer...

Page 13: ...t mode Offset Setup of the amount of compensation between the cover glass top surface and target position AF zone AF search zone AF center width and range In continuous focus mode Confirm that the cover glass top surface is detected in the one shot mode before switching to the continuous focus mode For details refer to the instruction manual and Help of the application When using the MetaMorph app...

Page 14: ...servations Field number 22 AF focusing speed One shot focus mode only Approximately 1 to 5 sec from the focusing range Excluding the offset drive time Note The AF speed may slow down depending on the PC setup AF repeatability 20X and 40X objectives 0 1 μm with constant temperature 0 17 μm with less than 5 C temperature change 60X objective or 40X 60X 100X oil water immersion objectives 0 07 μm wit...

Page 15: ...sh is focused Set the AF zone so that its center is located on the interface between the culture fluid and glass bottom dish and the AF zone width is 10 µm 10 b Autofocusing takes long time or fails AF is set improperly Set up AF properly For the setting method refer to the instruction manual for the application software 10 Electrical noise is interfering with op eration Ground the IX2 UCB Be also...

Page 16: ...2 Printed in Japan on May 19 2010 M 003 01 ...

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