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22

TROUBLESHOOTING GUIDE

Problem

Cause

Remedy

Page

29

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Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the
entire list, please contact your local Olympus representative for assistance.

1. Optical System

a) Bulb does not light.

Bulb is burned out.

Replace bulb.

Power cord is unplugged.

Plug power cord into the power outlet.

b) Bulb operates, but field of view re-

mains dark.

Aperture and field iris diaphragms are not
opened wide enough.

Adjust them to proper sizes.

c) Field of view is obscured or not

evenly illuminated.

Revolving nosepiece is not correctly en-
gaged.

Make sure that the revolving nosepiece
clicks properly into place.

Condenser top lens is not set correctly.

Swing out the top lens.

Condenser is not properly centered.

Center the condenser.

Turret is set in an intermediate position.

Set turret to a click position.

Field iris diaphragm is stopped down too
far.

Open the field iris diaphragm until it
circumscribes the field.

Bulb is not mounted correctly.

Push the pins of halogen bulb all the
way until the stop position.

d) Dirt or dust is visible in the field of

view.

Dirt/dust on the eyepieces.

Clean thoroughly.

Dirt or dust on condenser top lens, turret
upper surface covering glass

Dirt/dust on the specimen.

e) Visibility is poor.
 · Image is not poor.
 · Contrast is poor.
 · Details are indistinct.
 · Image glares.

A non-UIS2/UIS objective is used.

Use only UIS2/UIS series objectives
with this microscope.

Aperture iris diaphragm is stopped down
too far.

Open aperture iris diaphragm.

Correction collar on correction collar
equipped objective is not properly ad-
justed.

While focusing, turn the correction
collar to find the best position.

Front lens of objective is dirty.

Clean objective.

Immersion oil is not being used with an
oil immersion objective.

Use immersion oil.

Immersion oil contains bubbles.

Remove the bubbles.

Recommended immersion oil is not
used.

Use the provided immersion oil.

Dirt/dust on specimen.

Clean it.

Dirt/dust on condenser top lens, turret or
upper surface covering glass.

Inappropriate object side or cover glass
thickness.

Replace with glass of recommended
thickness.

3

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17

30

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Summary of Contents for BX45

Page 1: ...he safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this in...

Page 2: ......

Page 3: ...entering the Condenser 2 Compatibility of Objectives and Condensers 3 6 Immersion Objectives 17 1 Using Immersion Objectives 3 7 Objectives with Correction Collar 17 3 8 Marker U MARKER 18 4 OBSERVATI...

Page 4: ...7 OPTICAL CHARACTERISTICS 27 8 ASSEMBLY See this section for the replacement of the light bulb 28 30 PROPER SELECTION OF THE POWER SUPPLY CORD 31 32...

Page 5: ...NT SAFETY PRECAUTIONS Fig 1 Fig 2 This microscope employs a UIS2 UIS Universal Infinity System optical design and should be used only with UIS2 UIS eyepieces objectives and condensers for the BX2 seri...

Page 6: ...at parts where special precaution is required when handling and using the microscope Always heed the warnings Should warning stickers become soiled peeled off etc contact Olympus for replacement 1 A m...

Page 7: ...limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio freque...

Page 8: ...16 Y axis knob Page 12 X axis knob Page 12 Turret Page 5 Brightness adjustment knob Page 8 Lamp voltage adjustment knob Field iris diaphragm ring Page 15 Marker U MARKER Allen screwdriver accommodatio...

Page 9: ...Page 15 Turret View when disassembled from the microscope frame Aperture iris diaphragm Page 16 Filter mount Bottom side Page 9 Mountable filters 32 mm diameter 32C provided 32LBD 32LND6 32LND25 32IF...

Page 10: ...er X Y axis knobs Engage the 10X objective in the light path Then engage the top lens in the light path Revolving nosepiece Top lens swing out lever Bring the specimen in focus Coarse fine adjustment...

Page 11: ...BX45 7 Copy the observation procedure pages on separate sheets and post it near your microscope Trinocular observation tube only...

Page 12: ...switch lights when it is ON 2 Using a small flat blade screwdriver turn the preset adjustment screw to obtain the required light intensity Turning the screw clockwise increases brightness 3 When the...

Page 13: ...the bottom part are determined by the aperture iris diaphragm positioning and optical devices inserted in the upper part Examples Aperture iris 32C ND 32LBD Phase contrast PH 32IF550 Darkfield DFA No...

Page 14: ...ng the tension ad justment ring The coarse adjustment knob tension is preadjusted for easy use How ever if desired you can change the tension using the tension adjust ment ring Turning the ring in the...

Page 15: ...ent knob to raise the objective 2 Open the spring loaded curved finger on the slide holder and place one or two specimen slides on the stage from the front 3 After placing the sides as far as they wil...

Page 16: ...it hits the stoppers Rubber Caps for X and Y Axis Knobs Optional When the X and Y axis knobs are fitted with the rubber caps the knobs can be adjusted without slipping and fine adjustment is possible...

Page 17: ...rough the eyepiece without the diopter adjustment ring rotate the coarse and fine adjustment knobs to bring the specimen into focus 2 Looking through the eyepiece with the diopter adjustment ring turn...

Page 18: ...e peripheral sections of the field may be dark With the U ETBI U TTBI Fig 22 The U ETBI and U TTBI are ergonomic observation tubes with normal field capable of the tilting adjustment as well as the ad...

Page 19: ...e condenser is properly cen tered if the iris image is centered and inscribed in the field of view 7 During actual use open the field diaphragm slightly until its image cir cumscribes the field of vie...

Page 20: ...ture scale to about 80 of the NA value of the respective objective Fig 27 Example With the UPlanFLN40X NA 0 75 set the scale to 0 75 x 0 8 0 6 Aperture iris diaphragm image Fig 26 Fig 27 N A scale Obj...

Page 21: ...use immersion oil in the space between the top lens and specimen 4 After use remove immersion oil from the objective front lens by wiping with gauze slightly moistened with absolute alcohol If immersi...

Page 22: ...Remove the centering knobs and place the turret in the original posi tion Insert the turret in the direction of the arrow on the dovetail Fig 32 Mounting the marker on a 10X objective allows you to ma...

Page 23: ...sert the two centering screws into the optical device centering thread holes above the markings and adjust centering of the PH ring so that the bright ring overlaps the dark ring in the field Fig 34 I...

Page 24: ...look into the eyepiece sleeves to locate the objective pupil and turn the optical device centering screw holes above the markings to center the DF ring so that no light exits through the objective pup...

Page 25: ...tted brightfield observation light path 2 Engage the objective in the light path When a Ph objective is used the contrast may be weakened 3 Turn the polarizer lever slightly so that the field becomes...

Page 26: ...aphragm is stopped down too far Open the field iris diaphragm until it circumscribes the field Bulb is not mounted correctly Push the pins of halogen bulb all the way until the stop position d Dirt or...

Page 27: ...intensity Install bulb adjustment knob Light intensity preset switch is set to ON Press switch to OFF Bulb is not installed Install bulb Bulb is burned out Replace bulb Lamp socket is not connected Co...

Page 28: ...Place specimen correctly c X and Y axis knobs are too tight or too loose Tension of X and Y axis knobs is too high or too low Adjust tension d Stroke has reduced Stage guide is deviated Correct deviat...

Page 29: ...position revolving nosepiece fixed with a slider inlet 5 Observation tube Type U BI30 2 U TBI3 U ETBI U TTBI Widefield binocular Widefield tilting binocular Widefield erect inverted tilting binocular...

Page 30: ...NA in widefield observation is 22 in any mode 8 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperatures up to...

Page 31: ...27 2 5 5 10X 0 3 10 0 1 12 100X 22 4 2 2 20X 0 5 2 1 0 17 0 67 200X 7 0 1 1 40X 0 75 0 51 0 17 0 45 400X 2 52 0 55 40XO 1 3 0 2 0 17 0 26 400X 1 27 0 55 Oil immersion 60X 0 9 0 2 0 17 0 37 600X 1 5 0...

Page 32: ...or touching glass surfaces Assembly steps enclosed in will be detailed on the subsequent pages All assembly operations are possible by using the Allen screwdriver provided with the microscope ASSEMBL...

Page 33: ...th the socket gently push the lamp socket all the way into place Caution for Bulb Replacement During Use or Right After Use The bulb and the lamp socket are areas near these will be extremely hot duri...

Page 34: ...is instruction manual 1 Connect the power cord plug to the AC receptacle The power cord should be connected to a grounded 3 conductor power outlet If the power outlet is not grounded properly Olympus...

Page 35: ...ing Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The...

Page 36: ...ter sterreichischer Verband f r Elektrotechnik VE Danmarks Elektriske Materialkontroll DEMKO National Standards Authority of Ireland NSAI Norges Elektriske Materiellkontroll NEMKO Asociacion Electrote...

Page 37: ...MEMO...

Page 38: ...MEMO...

Page 39: ......

Page 40: ...2 Corporate Center Drive Melville NY 11747 3157 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 2 8 Honduras Street London EC1Y OTX United Kingdom 31 Gilby Road Mt Wav...

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