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BX45

15

Fig. 23

3-5 Condenser

@

²

Fig. 25

1

    Centering the Condenser

(Figs. 23 - 25)

Fig. 24

|

³

1. Turn the condenser height adjustment knob @ to raise the condenser to

its upper limit, then use the top lens swing-out lever ² move the top lens
into the light path.

2. Focus on the specimen using the 10X objective.
3. Rotate the field iris diaphragm ring ³ in the direction of the arrow so that

the diaphragm image comes inside the field of view.

4. Manipulate the condenser height adjustment knob @ to focus on the

diaphragm image.

5. Insert the two condenser centering screws | into the condenser center-

ing thread holes (below the   marking) and turn the screws to move the
iris diaphragm image to the center of the field of view.

6. Gradually open the field iris diaphragm. The condenser is properly cen-

tered if the iris image is centered and inscribed in the field of view.

7. During actual use, open the field diaphragm slightly until its image cir-

cumscribes the field of view.

}After completing the condenser centration, store the centering screws in

the accommodation positions on the right side of the microscope frame
(page 4) so as not to lose them.

Effects of Field Iris Diaphragm  (Fig. 25)

The field iris diaphragm restricts the diameter of the beam of light enter-
ing the objective and thus excludes extraneous light, improving image
contrast. The diameter of the field iris should e adjusted for objective
power to the extent that it just circumscribes the field of view. (See “Com-
patibility of Objectives and Condensers” on the next page.)

}With the 100X objective, the field iris diaphragm image cannot be ob-

served unless the iris diaphragm is minimized. With the 4X objective,
maximize the iris diaphragm to observe it.

Summary of Contents for BX45

Page 1: ...he safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the microscope Retain this in...

Page 2: ......

Page 3: ...entering the Condenser 2 Compatibility of Objectives and Condensers 3 6 Immersion Objectives 17 1 Using Immersion Objectives 3 7 Objectives with Correction Collar 17 3 8 Marker U MARKER 18 4 OBSERVATI...

Page 4: ...7 OPTICAL CHARACTERISTICS 27 8 ASSEMBLY See this section for the replacement of the light bulb 28 30 PROPER SELECTION OF THE POWER SUPPLY CORD 31 32...

Page 5: ...NT SAFETY PRECAUTIONS Fig 1 Fig 2 This microscope employs a UIS2 UIS Universal Infinity System optical design and should be used only with UIS2 UIS eyepieces objectives and condensers for the BX2 seri...

Page 6: ...at parts where special precaution is required when handling and using the microscope Always heed the warnings Should warning stickers become soiled peeled off etc contact Olympus for replacement 1 A m...

Page 7: ...limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio freque...

Page 8: ...16 Y axis knob Page 12 X axis knob Page 12 Turret Page 5 Brightness adjustment knob Page 8 Lamp voltage adjustment knob Field iris diaphragm ring Page 15 Marker U MARKER Allen screwdriver accommodatio...

Page 9: ...Page 15 Turret View when disassembled from the microscope frame Aperture iris diaphragm Page 16 Filter mount Bottom side Page 9 Mountable filters 32 mm diameter 32C provided 32LBD 32LND6 32LND25 32IF...

Page 10: ...er X Y axis knobs Engage the 10X objective in the light path Then engage the top lens in the light path Revolving nosepiece Top lens swing out lever Bring the specimen in focus Coarse fine adjustment...

Page 11: ...BX45 7 Copy the observation procedure pages on separate sheets and post it near your microscope Trinocular observation tube only...

Page 12: ...switch lights when it is ON 2 Using a small flat blade screwdriver turn the preset adjustment screw to obtain the required light intensity Turning the screw clockwise increases brightness 3 When the...

Page 13: ...the bottom part are determined by the aperture iris diaphragm positioning and optical devices inserted in the upper part Examples Aperture iris 32C ND 32LBD Phase contrast PH 32IF550 Darkfield DFA No...

Page 14: ...ng the tension ad justment ring The coarse adjustment knob tension is preadjusted for easy use How ever if desired you can change the tension using the tension adjust ment ring Turning the ring in the...

Page 15: ...ent knob to raise the objective 2 Open the spring loaded curved finger on the slide holder and place one or two specimen slides on the stage from the front 3 After placing the sides as far as they wil...

Page 16: ...it hits the stoppers Rubber Caps for X and Y Axis Knobs Optional When the X and Y axis knobs are fitted with the rubber caps the knobs can be adjusted without slipping and fine adjustment is possible...

Page 17: ...rough the eyepiece without the diopter adjustment ring rotate the coarse and fine adjustment knobs to bring the specimen into focus 2 Looking through the eyepiece with the diopter adjustment ring turn...

Page 18: ...e peripheral sections of the field may be dark With the U ETBI U TTBI Fig 22 The U ETBI and U TTBI are ergonomic observation tubes with normal field capable of the tilting adjustment as well as the ad...

Page 19: ...e condenser is properly cen tered if the iris image is centered and inscribed in the field of view 7 During actual use open the field diaphragm slightly until its image cir cumscribes the field of vie...

Page 20: ...ture scale to about 80 of the NA value of the respective objective Fig 27 Example With the UPlanFLN40X NA 0 75 set the scale to 0 75 x 0 8 0 6 Aperture iris diaphragm image Fig 26 Fig 27 N A scale Obj...

Page 21: ...use immersion oil in the space between the top lens and specimen 4 After use remove immersion oil from the objective front lens by wiping with gauze slightly moistened with absolute alcohol If immersi...

Page 22: ...Remove the centering knobs and place the turret in the original posi tion Insert the turret in the direction of the arrow on the dovetail Fig 32 Mounting the marker on a 10X objective allows you to ma...

Page 23: ...sert the two centering screws into the optical device centering thread holes above the markings and adjust centering of the PH ring so that the bright ring overlaps the dark ring in the field Fig 34 I...

Page 24: ...look into the eyepiece sleeves to locate the objective pupil and turn the optical device centering screw holes above the markings to center the DF ring so that no light exits through the objective pup...

Page 25: ...tted brightfield observation light path 2 Engage the objective in the light path When a Ph objective is used the contrast may be weakened 3 Turn the polarizer lever slightly so that the field becomes...

Page 26: ...aphragm is stopped down too far Open the field iris diaphragm until it circumscribes the field Bulb is not mounted correctly Push the pins of halogen bulb all the way until the stop position d Dirt or...

Page 27: ...intensity Install bulb adjustment knob Light intensity preset switch is set to ON Press switch to OFF Bulb is not installed Install bulb Bulb is burned out Replace bulb Lamp socket is not connected Co...

Page 28: ...Place specimen correctly c X and Y axis knobs are too tight or too loose Tension of X and Y axis knobs is too high or too low Adjust tension d Stroke has reduced Stage guide is deviated Correct deviat...

Page 29: ...position revolving nosepiece fixed with a slider inlet 5 Observation tube Type U BI30 2 U TBI3 U ETBI U TTBI Widefield binocular Widefield tilting binocular Widefield erect inverted tilting binocular...

Page 30: ...NA in widefield observation is 22 in any mode 8 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperatures up to...

Page 31: ...27 2 5 5 10X 0 3 10 0 1 12 100X 22 4 2 2 20X 0 5 2 1 0 17 0 67 200X 7 0 1 1 40X 0 75 0 51 0 17 0 45 400X 2 52 0 55 40XO 1 3 0 2 0 17 0 26 400X 1 27 0 55 Oil immersion 60X 0 9 0 2 0 17 0 37 600X 1 5 0...

Page 32: ...or touching glass surfaces Assembly steps enclosed in will be detailed on the subsequent pages All assembly operations are possible by using the Allen screwdriver provided with the microscope ASSEMBL...

Page 33: ...th the socket gently push the lamp socket all the way into place Caution for Bulb Replacement During Use or Right After Use The bulb and the lamp socket are areas near these will be extremely hot duri...

Page 34: ...is instruction manual 1 Connect the power cord plug to the AC receptacle The power cord should be connected to a grounded 3 conductor power outlet If the power outlet is not grounded properly Olympus...

Page 35: ...ing Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The...

Page 36: ...ter sterreichischer Verband f r Elektrotechnik VE Danmarks Elektriske Materialkontroll DEMKO National Standards Authority of Ireland NSAI Norges Elektriske Materiellkontroll NEMKO Asociacion Electrote...

Page 37: ...MEMO...

Page 38: ...MEMO...

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Page 40: ...2 Corporate Center Drive Melville NY 11747 3157 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 2 8 Honduras Street London EC1Y OTX United Kingdom 31 Gilby Road Mt Wav...

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