OligoMaker DNA/RNA Synthesizer
OligoMaker ApS
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Kong Georgs Vej 12
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DK-2000 Frederiksberg
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Tel: +45 321 322 00
www.oligomaker.com
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email:
p.17
9.2 Check Reagents
It is indicated on the display how much reagent is required during synthesis. Make a
visual check on the reagent bottles to verify the amount of reagent which is available.
The numbers can vary and has to be adjusted according to the real use (adjusted on
Synthesis Report, delivered seperately).
On the Opening screen, press ”Prime” on the display. Make sure you have activated the
pressure to be able to prime. Activate the pressure for each reagent and shut down the
pressure to the bottles afterwards. When the pressure is off again, the pressure on P1
and P2 should not fall more than 0,2 during a period of two min. P3 indicates the
pressure on the top gas.
Manometer
Vac
Vacuum when pumping
P1
Pressure on bottles Wash, Deblock, Cap A, Cap B and Oxidizer
P2
Pressure on bottles Activator and Amidites
P3
Pressure on Top gas