manualshive.com logo in svg

Ocean Optics CHEM2000, Operating Manual And User Manual

Share
Download
Ocean Optics CHEM2000 Operating Manual And User Manual Download Page 42

38

Independent

Dependent

Values computed from

Variable

Variables

the regression output

True

Wavelength

(nm)

Pixel #

Pixel # 

2

Pixel # 

3

Predicted

Wavelength

Difference

253.65

296.73

302.15

313.16

334.15

365.02

404.66

407.78

435.84

546.07

576.96

579.07

696.54

706.72

727.29

738.40

751.47

175

296

312

342

402

490

604

613

694

1022

1116

1122

1491

1523

1590

1627

1669

30625

87616

97344

116964

161604

240100

364816

375769

481636

1044484

1245456

1258884

2223081

2319529

2528100

2647129

2785561

5359375

25934336

30371328

40001688

64964808

117649000

220348864

230346397

334255384

1067462648

1389928896

1412467848

3314613771

3532642667

4019679000

4306878883

4649101309

253.56

296.72

302.40

313.02

334.19

365.05

404.67

407.78

435.65

546.13

577.05

579.01

696.70

706.62

727.24

738.53

751.27

0.09

0.01

-0.25

0.13

-0.05

-0.04

-0.01

0.00

0.19

-0.06

-0.09

0.06

-0.15

0.10

0.06

-0.13

0.19

4.

 

Now you are ready to calculate the wavelength calibration coefficients. In your spreadsheet program,
find the functions to perform linear regressions.

if using Quattro Pro, look under 

Tools | Advanced Math

if using Excel, look under 

Analysis ToolPak

5.

 

Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number
squared and the pixel number cubed as the independent variables (X). After you execute the
regression, an output similar to the one shown below is obtained.

Regression Statistics

Multiple R

0.999999831

R Square

0.999999663

Adjusted R Square

0.999999607

Standard Error

0.125540214

Observations

22

intercept

Coefficients

Standard Error

Intercept

190.473993

0.369047536

first coefficient

X Variable 1

0.36263983

0.001684745

X Variable 2

-1.174416E-05

8.35279E-07

X Variable 3

-2.523787E-09

2.656608E-10

second coefficient

third coefficient

6.

 

The numbers of importance are indicated in the above figure. You will need to record the Intercept as
well as the First, Second, and Third Coefficients. Also, look at the value for R squared. It should be

very

 close to 1. If it is not, you have probably assigned one of your wavelengths incorrectly.

7.

 

Select 

Spectrometer | Spectrometer Configuration

 from the menu. Update the wavelength coefficients.

Summary of Contents for CHEM2000

Page 1: ...niature Fiber Optic Spectrophotometers Ocean Optics Inc 380 Main Street Dunedin FL 34698 727 733 2447 727 733 3962 fax For the latest information consult our web site www OceanOptics com Or e mail our...

Page 2: ...without the prior consent of Ocean Optics Inc in any form of binding or cover other than that in which it is published Trademarks Microsoft Windows Windows 95 Windows 98 and Windows NT are either reg...

Page 3: ...inetics Values 14 Printer Setup 14 Print Spectra and Kinetics 14 Exit 14 Edit Menu Functions 15 Clear Spectrum Overlays 15 Clear Kinetics Values 15 Autoscale X 15 Autoscale Y 15 Show Kinetics Values 1...

Page 4: ...s 28 Reflection Experiments 29 Relative Irradiance Experiments 30 Concentration Experiments 31 Kinetics Experiments 33 Appendix A Changing the Settings on the PC2000 34 Appendix B PC2000 Pin outs and...

Page 5: ...ber Optic Spectrometer nearly a thousand times smaller and ten times less expensive than previous systems Due to this dramatic reduction in size and cost of optical sensing systems applications once d...

Page 6: ...is our standard Windows operating software for the CHEM2000 and CHEM2000 UV VIS systems It is a basic acquisition and display software that provides a real time interface to a variety of spectral pro...

Page 7: ...l find your spectrometer in a silver gray anti static bag Your software diskettes and Wavelength Calibration Report are packaged with this manual A Wavelength Calibration Report comes with every spect...

Page 8: ...able I O Range Base Address 2 Note these available settings When you first run OOIChem you must enter these values in the Configure Hardware dialog box 3 Turn off the computer and take off the compute...

Page 9: ...o the spectrometer for synchronizing data with an external event Graph and chart display mode Choose Spectrum Only to only view live spectra from one spectrometer channel Choose Spectrum Kinetics to v...

Page 10: ...ne the values These default values are set in the operating software as well Most of the time these default settings will work with your computer However if you have many devices installed in your com...

Page 11: ...assigned a unique IRQ Install the PC2000 1 Turn off the computer and remove the computer cover 2 Ground yourself to the computer chassis or power supply 3 Remove the PC2000 spectrometer A D card comb...

Page 12: ...opens 1 Under Spectrometer Type choose S2000 PC2000 2 Under A D Converter Type choose ADC1000 PC2000 3 Under Base Address choose the same setting as the dip switches on the A D converter and the same...

Page 13: ...lows users to monitor and report up to 4 single wavelengths or up to 2 calculated values from a combination of wavelengths for example an absorbance value of 400 minus an absorbance value of 700 A kin...

Page 14: ...actors the intensity of the light source the reflectivity of the grating and the mirrors in the spectrometer the transmission of the fibers the response of the detector and the spectral characteristic...

Page 15: ...ned The plot is then used to determine the unknown concentrations of solutions See the Experiment Tutorial beginning on page 27 The absorbance of a solution is related to the concentration of the spec...

Page 16: ...r the signal to noise ratio The S N improves by the square root of the number of scans averaged Boxcar Smooth Enter a value to implement a boxcar smoothing technique that averages across spectral data...

Page 17: ...just the spectrum section of the graph If the function is enabled choosing the scan button results in the collection of data in both the spectrum section of the graph and the kinetics section of the...

Page 18: ...le Open Spectrum Overlay from the menu to open a dialog box that allows you to open a previously saved spectrum and to open it as an overlay a static spectrum while still acquiring live data You can o...

Page 19: ...Spectrometer Spectrometer Configuration from the menu and make sure that Spectrum Kinetics is selected next to Graph and chart display mode Then configure your experiment by selecting Spectrometer Kin...

Page 20: ...of light absorbed are constants Then a linear plot from taking these scans is obtained This Calibration Curve is used to determine the unknown concentrations See pages 31 32 for step by step instructi...

Page 21: ...ge ensures that the spectrometer will receive the appropriate voltage for the trigger event 2 Supply a line from Pin 8 of the J2 Accessory Connector to your triggering device See figure for pin locati...

Page 22: ...h you wish to collect data You can collect data from up to 4 single wavelengths characterized as a b c and d Display If you want the data graphed from these single wavelengths or from the mathematical...

Page 23: ...ould ignite flammable materials that come in contact with the metal housing There is an exposed fan on the bottom of the light source sample holder Handle with care Operation Adjusting the Fit of the...

Page 24: ...also hold the two front legs in place Once the screws are loosened gently pull the fan along with the legs away from the lamp 4 Now that the fan is removed use an Allen wrench to loosen the set screw...

Page 25: ...s Bulb life 900 hours Bulb color temperature 3100K Output to bulb 5 volts 1 3 amps Output regulation 0 2 voltage Time to stabilized output 30 minutes The useable range of the ISS 2 is limited to the w...

Page 26: ...light is being transmitted Bending the fiber will cause attenuation To minimize this effect add extra strain relief to both ends of the fiber Do not exceed the temperature specifications for the mate...

Page 27: ...ook directly into the light source sight down the beam into the source or stare at the diffuse reflected beam Dangerous voltages present NO serviceable parts inside unit To replace bulbs contact Ocean...

Page 28: ...rn the light source in the ISS UV VIS on and off 4 When you want to control the ISS UV VIS through our more advanced OOIBase32 Spectrometer Operating software select or deselect the Strobe Enable box...

Page 29: ...Deuterium bulb life 800 hours Tungsten bulb life 2 000 hours Ignition delay 1 5 seconds to 60 seconds Peak to peak stability 0 3 in 30 minutes 1 0 in 10 minutes Connector SMA 905 Power input 12 V Powe...

Page 30: ...re hydrogen loaded but their solarization resistant properties do not last as the hydrogen eventually leaks out of the silica meaning that these hydrogen doped solarization resistant fibers have a she...

Page 31: ...absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum yo...

Page 32: ...he reference reading by clicking the Reference button in the software display area This command merely stores a reference spectrum To save a spectrum you must select File Save Spectral Values from the...

Page 33: ...blocking the light path going to your spectrometer The analyte you want to measure must be absent while taking a reference spectrum Take the reference reading by clicking the Reference button in the s...

Page 34: ...r temperature in Kelvin of the reference lamp you are going to use is entered here The color temperature of the tungsten halogen light source that came with your CHEM2000 has a color temperature of 31...

Page 35: ...hile still in Scope Mode take a dark spectrum by first completely blocking the light path going to your spectrometer If possible do not turn off the light source If you must turn off your light source...

Page 36: ...you may also select a label for your concentration values such at Moles per Liter in the Concentration Units box This is only a label and does not affect the data in any way 14 Select Edit Show Legend...

Page 37: ...ted in a kinetics process Be sure to select hours minutes and seconds Data from a timed acquisition is stamped with a time that is accurate to 1 millisecond 5 Under Wavelength enter the single wavelen...

Page 38: ...f 768 A few of the many combinations for Base Address settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See page...

Page 39: ...to 7 Other combinations for IRQ settings are below After you have changed the switches reinstall the card and change the software settings to match the hardware settings See pages 6 8 for instructions...

Page 40: ...er Pin Function 1 Channel 1 slave 1 2 Channel 2 3 Channel 3 4 Channel 4 5 Channel 5 6 Channel 6 7 Channel 7 8 GND 9 GND 10 S0 11 Reserved 12 Multiple Strobe 13 Single Strobe 14 Reserved 15 F read ROG...

Page 41: ...ither a spreadsheet program Excel or Quattro Pro for example or a calculator that performs third order linear regressions If you are using Microsoft Excel choose Tools Add Ins from the menu and check...

Page 42: ...ind the functions to perform linear regressions if using Quattro Pro look under Tools Advanced Math if using Excel look under Analysis ToolPak 5 Select the true wavelength as the dependent variable Y...

Reviews:

No comments

Brands by name

Popular brands

Load more brands