NuAire NU-5710 Operation And Maintenance Manual Download Page 1

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OM0249

Page 1 of 64

Rev 4 June/2020

Air-Jacketed Automatic CO

Incubator

Models

NU-5710, NU-5720, NU-5731, NU-5741

NU-5710E NU-5720E, NU-5731E, NU-5741E

Operation and Maintenance Manual

November 2018

Revision 4

Series 3

For 115 Vac, 50/60 Hz Only

Manufactured By:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: (763)-553-0459

Summary of Contents for NU-5710

Page 1: ...0 NU 5720 NU 5731 NU 5741 NU 5710E NU 5720E NU 5731E NU 5741E Operation and Maintenance Manual November 2018 Revision 4 Series 3 For 115 Vac 50 60 Hz Only Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 ...

Page 2: ...eter Heater Adjustments Affecting Temperature Calibration 9 3 Setting Air Injections 9 4 CO2 Calibration 9 5 RH Calibration 9 6 O2 Calibration Section No 10 Maintaining Your DHD Invitrocell 10 1 Heated Decon Cycles 10 2 Chemical Decontamination 10 3 Power Loss recovery mode 10 4 Shutting down the incubator 10 5 Automatic Filter Change Notification Section No 11 Error Indicators Troubleshooting Sec...

Page 3: ...ors that measure CO2 volume as a percentage in the chamber for monitoring and control 1 3 Incubator Control Electronics The NuAire Incubator Control Electronics is a state of the art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment providing optimum programmable conditions for culture growth The microcomputer is user f...

Page 4: ...ns are settable by the user to help control condensation See section 8 8 for this option Models NU 5720 and NU 5741 use a humidity monitoring control system for chamber humidity the system is external to the chamber The chamber air is routed through the water vapor saturated air in an evaporator box to add humidity when the RH sensor indicates that more humidity is required maintain a set point 1 ...

Page 5: ... CAUTION Safety alert symbol indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury WARNING Note Biohazard Hazardous Gases Personal Protection Equipment Required Chemical Hazard Flammable Hazard Lead Free CAUTION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property damage Potent...

Page 6: ...er Most electronics motors pumps and valves are fully accessible from the back of the unit Access to the filters and CO sensor through the front of the incubator A CO2 sample port is provided on the front panel just below the door to check the concentration of CO2 in the chamber The CO2 percentage is controlled by a solid state gas infrared sensor using a single light source with dual wave length ...

Page 7: ...iters 3 3 Performance Parameters Temperature Control Available on All Models Performance Determined with O Set Point 21 0 Temperature Range 5 C to 55 C 37 C default 5 C above ambient to 30 C maximum ambient Temperature Uniformity 0 3 C 37 C Temperature Accuracy 0 1 C Temperature Recovery 0 12 C minute Average Temperature Display Resolution 0 1 C Temperature Sensor Type Precision Integrated Circuit...

Page 8: ...rm RS 485 2way communication and 4 20 mA analog output signals for system performance Note Performance ratings apply ONLY when the Incubator is installed and set up properly per the instructions in the manual has stabilized at all set points and is in a stable environment See sections 8 and 9 for proper set up While idle a system will not inject gas or RH and will not alarm 3 4 Standard Items Pack...

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Page 10: ... the green bonding conductor of the supply cord and any dead metal part of the cabinet shall not exceed 0 10 ohms 4 3 Functional Tests These functional tests are performed on every Incubator over a minimum of a 48 hour burn in period The results are recorded on the final Inspection report and a copy is included with each incubator that is shipped All failures are noted corrected and any failed tes...

Page 11: ... 6 1 Damaged Shipments 6 1 1 Terms are F O B factory unless stated otherwise Therefore it is important to check each shipment before acceptance 6 1 2 If there is visible damage the material can be accepted after the driver makes a notation on the consignee s copy of the freight bill Then an inspection must be made to verify the claim against the carrier This inspection is the basis of your filing ...

Page 12: ...nging door Note When locating Incubators side by side they should be spaced at least 10 apart to permit one Incubator to be in a heated Decon Cycle with a minimal heating disturbance on adjacent Incubators running in their normal run mode 7 2 Leveling Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber The Incubator should have all 4 leveling feet fir...

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Page 14: ...he air inlet on the back of the incubator is connected to the filter by a hose at the factory and does not require installation CAUTION This is a free air supply DO NOT connect to a pressurized air source 7 6 CO2 N2 Gas Supply Setup 7 6 1 Gas and Air supply HEPA filtering is installed at the factory on the front of the incubator as illustrated in the picture Each incubator is equipped with the fil...

Page 15: ...ave two gauges USE A TWO STAGE REGULATOR All NuAire Incubators use CO2 N2 in such quantities that precise metering of CO2 N2input pressure is important for maximum performance To connect the regulator First Open the CO2 cylinder slightly for an instant this is termed cracking the valve This will blow out dust or dirt that may have collected in the valve outlet BE SURE to keep your face away from t...

Page 16: ...he Incubator is installed correctly connected to the power source it is rated for gas connections made properly the water pan filled the shelves in place and the unit is leveled Read section 8 and follow all instructions for setting set points at the desired value Then calibrate the Incubator control systems as explained in section 9 If the Incubator is installed and calibrated correctly it will m...

Page 17: ...hieved through the process of water evaporation vapor water pressure from a stainless steel water pan placed on the bottom of the chamber Materials of different thermal resistance e g glass plastic do not offer sufficient thermal recovery and are not recommended for water pan usage Although some metals offer better thermal coefficients than stainless steel dissimilar metals cause electrolysis in t...

Page 18: ...ng the recovery time to 90 5 3 is 25 minutes on average The control system has a 3 accuracy from any given set point 90 and below Much of the information regarding the water pan can be applied with the exception of the water pan The control system uses a solid state capacitance humidity sensor to monitor the relative humidity within the chamber If humidity is required the control system will activ...

Page 19: ...sing the Incubator Control Screens Accessing the many Control screens performing functions and diagnostic tests and maneuvering through the NuTouch LCD menu system is accomplished through the following methods Touching a graphic Icon like the NuAire Logo in the Main Screen which access the System Settings menu or lead you to an Enter Password screen when a password has been established Touching a ...

Page 20: ...y touching any control system numerical value in the MAIN SCREEN Performance Monitor Views a function s Set Point and Measured values over the last seven days Service Settings Protected by the password 9876 it facilitates Calibrations Option settings and access to Diagnostics System Information Views the units Model Number Serial Number Control board assembly CCBA Software Revision and the Display...

Page 21: ...omize incubator performance perform system diagnostics and to Factory or Master reset for returning the incubator to Default settings 2 A selectable 4 digit numerical password that is entered by the Incubator user to prevent others that are not authorized to change the Incubator set points CO2 Temp running conditions of the Incubator initiate a Decon Cycle or changing passwords The password protec...

Page 22: ...g the top middle of the front panel After the door is closed the text will display Door Closed Delay indicating the delay time 60 sec adjustable 8 4 6 USB status The USB status icon appears in the lower right side of the Main screen when a memory stick is placed in the USB port to the right of the NuTouch display while down loading performance and event history This icon disappears when the memory...

Page 23: ...ent O2 percentage readings 8 5 4 CO2 Inlet All models The CO2 inlet provides a fitting for vinyl tubing Be sure to follow the recommended inlet pressure to insure proper flow rates and consistent CO2 percentage readings 8 5 5 Power Switch The power switch located at the top of the rear panel controls all power to the Incubator 8 5 6 Standard Communication Interface s Alarm Three receptacle connect...

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Page 25: ...instructions found in Maintenance Section 10 8 6 3 CO2 Supply Filter The CO2 supply filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10 8 6 4 Air Inlet Filter All models The air inlet filter is installed at the factory and should be changed following the instructions found in Maintenance Section 10 This is a free air supply Note DO NO...

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Page 27: ... interaction is required when the incubator is first turned on then to perform calibration functions and address abnormal conditions status If an abnormal condition has or does exist for a particular parameter an alarm becomes active and the Alarm Status button will appear This could be a catastrophic alarm condition which could harm the tissue culture cells It does indicate an operational abnorma...

Page 28: ...h achieving these lower O set points NU 5731 and NU 5741 8 8 1 4 Automatic Adjustment of Post Inject Delay Times when O control system is active Post inject delays are automatically adjusted according to the O set point to facilitate gas and RH recovery after a door opening The value displayed in the option menu will not change but is the basis for a formula in the software that calculates the new...

Page 29: ...stic Mode To initiate the diagnostic mode perform the following from the Main screen Select the NuAire Logo to enter System Settings Select Service Settings and enter 9876 and then hit enter ENT In the Service Settings menu select the Diagnostics button for the desired function Output Input Diagnostic Parameter Functions Temperature 1 Safety Relay on off 2 Chamber Temp Sensor shows current temp 3 ...

Page 30: ...e turned on at different percentages 0 50 and 100 percent The LCD display will show phase firing percentage chosen default is 0 6 Perimeter Heater This function shows the current state of the perimeter heater This function also allows the perimeter heater to be turned on at different percentages 0 50 and 100 percent The LCD display will indicate percentage of phase firing chosen default is 0 CO2 C...

Page 31: ...Un Calibrated sensor signal 2 N2 Inject Valve on off This function indicates the on off default off state of the N2 Inject solenoid valve and allows the activation of it 3 O2 Bypass valve on off THIS FEATURE IS NOT ACTIVE 4 Blocking Solenoid on off This function indicates the on off default off state of the Blocking solenoid valve and allows the activation of it General Control Diagnostics 1 Power...

Page 32: ...Low Time Out Timer 10 minutes 0 240 4 Minimum Below Set Point 0 5 1 10 0 5 Post Inject Delay Time PIDT 60 seconds 0 250 Relative Humidity 1 System enable On On Off in Settings Screen 2 Maximum above Set Point 8 0 0 0 30 0 3 RH Low Time Out timer 60 minutes 0 300 4 Minimum below Set Point 3 0 0 1 15 0 5 Post Inject Delay time PIDT 90 seconds 0 300 O2 1 System Enable On On Off in Settings Screen 2 M...

Page 33: ...the chamber heaters in half degree increments during a temperature recovery cycle The delay time is required to prevent temperature overshoot of the control set point CO2 1 CO System Enable Enabled disabled Is located in the bottom right of the screen This function will enable or disable the CO system If the system is Enabled it will be displayed in the MAIN screen If it is disabled it will not be...

Page 34: ... chamber O 1 System Enable Enabled disabled on models NU 5731 and NU 5741 Is located in the bottom right of the screen This function will enable or disable the O system If the system is Enabled it will be displayed in the MAIN screen If it is disabled it will not be displayed in the Main or Option Settings screen for the O system System Enabled with check mark means the System is enabled System En...

Page 35: ...8 0 disables the feature View time remaining until current notice is triggered The Clear Password button that clears the 4 digit password that was input through the Set Password screen Change language Button Pressing this button brings up a screen with the language choices of English default Spanish French German Pressing the button in that screen of the language desired will change the user scree...

Page 36: ...on is correct you can press Save which will save the information displayed in the screen then take you to the date and time set screen If the date and time are correct you can press exit and go to the MAIN screen If you press Exit you will go to the main screen however if there is a power cycle the incubator will reboot to the Factory set up screen again All setup information will show correctly b...

Page 37: ...key pad screen and you will return to the Factory Set Up screen showing the serial number you just entered Default is blank if no number is entered Touch the Set Language text then touch the save button This sets the language on all of the user screens The language in the Service Settings menu screen will remain in English English is default language if none is chosen Touch the green save button t...

Page 38: ...alibration button Determine actual temperature within chamber by reading temperature measurement instrument Select the number digits below the Current Calibrated Sensor Reading blue text button Input the current value displayed on your temperature measurement instrument and exit calibration Note THE DISPLAY DOES NOT UPDATE IMMEDIATELY IT TAKES 2 3 SECONDS The chamber temperature calibration is com...

Page 39: ...re 50 and 20 Press Done to set current value and exit calibration The reset button for both the door and the perimeter heaters resets the number displayed to 0 0 removing any adjustment previously made Adjusting Door and perimeter heater duty cycles to control condensation Location of condensation Adjustment On the inner glass door Increase the door heater duty cycle in increments no greater than ...

Page 40: ... enter the CO2 Calibrations menu Press the CO2 Offset Calibration text button Use an independent instrument to determine actual CO2 percentage compare the display CO2 to the independent measurement If these two readings have a difference equal to or less than 0 5 percent proceed to enter the CO2 Fyrite value This calibration only allows a maximum of 0 5 offset See Note below If the difference is g...

Page 41: ...ssure of the Incubator is changed or is the CO2 flow control valve is disturbed The following steps should be taken for the CO2 injection calibration Enter CO2 Calibrations by means of the Service Settings menu Select the CO2 Injection Time text Follow the on screen instructions 9 5 Relative Humidity sensor calibration System available on Models NU 5720 and NU 5741 Relative humidity calibration ca...

Page 42: ...en to input the independently measured value and press the ENT button on the key pad This returns you to the Calibrate RH offset screen I might take a couple of seconds for the new value to appear in this screen Then press the SAVE button to return to the Service settings screen Pressing the exit button in that screen will return you to the MAIN screen NOTES 1 When you use the Water pan method of ...

Page 43: ... side of filter when replacing filter Sensor Bay Capsule Filter P N RF566 Capsule Uni Directional In Line Wet This filter should be replaced EVERY TWO YEARS to assure optimum performance A visual check should be performed when maintenance reminder appears in the main screen to assure filter integrity Remove sensor bay cover to perform visual check Air Supply Filter P N X 980385 50 mm Disk Uni dire...

Page 44: ...INATION CYCLE OR IF CHAMBER TEMPERATURE IS OVER 55 THE DECONTAMINATION CYCLE WILL HEAT THE INCUBATOR INTERIOR SURFACES TO 145 C CONTACT WITH ANY SURFACE INSIDE OF THE OUTER DOOR DURING THIS CYCLE CAN RESULT IN BURNS PREPARATION Following the prompts in the incubator display screen you will Remove culture cells samples dishes instruments or other user introduced equipment from the chamber Wiping ou...

Page 45: ...p portion of the cycle Temperature display shows chamber temperature Text displays that the unit is currently in the Heating Up cycle A timer tracks how long the unit has been heating thus far DECONTAMINATION 3 hours The unit automatically starts a countdown timer for the correct length of cycle when the decontamination temperature is reached Temperature display shows chamber temperature Text disp...

Page 46: ...our cycle or if the temperature drops more than 10 it will go back to the heat up phase then resume the cycle when decon temperature is reached It is considered normal for some odor to occur during the Decon Cycles This odor reduces with use of the Decon Cycle Some discoloration due to heat of the chamber and its components can be expected If decon temperature is not reached within the time allott...

Page 47: ... with a disinfectant then fill with 300ml of water Place it back in the chamber on the highest shelf for the decon cycle FOLLOW ALL FEDERAL STATE AND LOCAL REGULATIONS THAT APPLY TO THE DISINFECTANT USED TO CLEAN THE CHAMBER Note CO2 RH and O systems will be idle during the cycle Protection of the CO2 and O sensors during the heated cycle is automatic RH sensor must be removed from all models equi...

Page 48: ...phase Temperature display shows chamber temperature during cycle The Text displays that the Decontamination cycle is in progress A timer tracks the remaining time in Decontamination COOL DOWN 3 hours When the humidity reduction period is complete the heaters are shut off to cool the chamber to the user chosen temperature set point Temperature display shows chamber temperature during cycle The Text...

Page 49: ...e meaning they will not control or alarm while in this mode o Power Loss Recovery shows in place of RUN in the main screen o Heaters and Air Pump run as normal Once entered this mode will run for 1 hour A count down timer screen see below can be found when pressing the CO2 or O2 Diagnostics button in the Service Settings screen It is also recorded in the event log 10 4 Shutting down the Incubator ...

Page 50: ...This is recommended Change the timer by sliding the green button right to decrease or left to increase the amount of time until the next notification The number of months showing will change as the button is moved Press the Save button to save the timer setting and go the General Setting screen Pressing the Exit button will not save the new timer setting Setting the timer to 0 months deactivates t...

Page 51: ...ors can be cleared by pressing the Alarm Status button and entering the Alarms Menu and pressing the Clear All button if the error is still present the indicator will return when the alarm time reaches 0 Step 3 MONITOR REOCCURRENCE OF ERROR INDICATORS If reoccurrence of the error indicator is immediate or daily use the Error Indicator Troubleshooting Guide on the following pages to address the iss...

Page 52: ... in at board connector P6 Replace CO2 shroud temperature sensor Sensor Bay Temp Low Sensor bay is below temp set point 2 See CO2 diagnostics screen Check control board output to relay replace board if faulty Check that all connections are secure Check sensor bay heater for function replace if faulty Sensor Bay Temp High Sensor bay temp is above 65 deg C Check control board output to relay replace ...

Page 53: ...oid for loose connections Evaporator Temp low Water temperature in the water tank is below the alarm limit for 120 minutes Check for loose connections on the tank heater Check for loose connections on the tank temperature sensor Check the tank heater for function replace if faulty O2 System Displayed Error Code Code Description Checks and Corrections O2 Under Set Point O2 under set point Perform O...

Page 54: ...wer Failure Low voltage detected on the 12 VDC power circuit Check output Power Supply If good could be Control Bd Check power supply connection to Control board DC power supply failed replace 12 VDC Power Failure Low voltage detected on the 12 VDC power circuit Check output Power Supply If good could be Control Bd Check power supply connection to Control board DC power supply failed replace 5 VDC...

Page 55: ...n 10 4 for more detail on all responses Message is RH Watertank Fill with maximum 3 liters of distilled single water no purer Than 1 Mega Ohm removes message on main screen For further assistance call NuAire Customer Service at 1 800 328 3352 or 763 553 1270 USA 12 0 Communication Outputs 12 1 Remote Alarm Contacts The NuAire DHD Incubator contains a set of contact points to connect to a remote al...

Page 56: ...d its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply 13 5 Pollution Degree 2 0 Pollution degree describes the amount of ...

Page 57: ...ed Component Material Incubator Cabinet Painted Steel Outer Door Painted Steel Chamber Stainless Steel Outer Door Gasket Vinyl Clad Magnet Inner Door Gasket Silicon Rubber Front Service Panel Painted Steel Electronics Panel Painted Steel Sensor Bay Aluminum Exhaust Filter Aluminum Hosing Silicon Rubber Tubing Stainless Steel Air Pump Various Steel Copper Nylon Motor Various Steel Copper PVC Printe...

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