ELC-01MX User Manual
version 2.6
page 21
b) For simulation of an experiment using a sharp electrode
❏
Connect the BNC jack labeled 50 M
Ω
of the cell model to the BNC connector P
EL
at the
headstage.
For a) and b)
❏
Connect GND of the cell model to GND of the headstage.
Important: When using a differential headstage (optional) the REF connector at the
headstage must not be left open. It must be connected to ground.
Simulation of electrode in the bath
❏
Set switch #4, Figure 8 to the lower position.
❏
Set switch #5, Figure 8 to GROUND position. The 1 k
Ω
resistor simulates the resistance
of the bath solution. This can be used to train cancellation of offsets, using the bridge
balance and using the capacity compensation.
Simulation of SEAL formation
❏
Set switch #4, Figure 8 to the lower position.
❏
Set switch #5, Figure 8 to SEAL position. The 1 G
Ω
resistor simulates the SEAL
resistance when forming a GIGASEAL in patch clamp experiments.
Simulation of intracellular recording
Intracellular recordings can be mimicked with one of two cells with different properties. Use
the 50 M
Ω
electrode connector (#1, Figure 8) for an experiment with sharp electrodes or the
10 M
Ω
electrode connector (#3, Figure 8) for simulating an experiment with patch electrodes.
❏
Switch the CELL membrane switch (see #4, Figure 8) to the desired position (CELL 1 or
CELL 2).
❏
Turn all controls at the amplifier to low values (less than 1) and the OFFSET in the range
of 5 (zero position) and the OSCILLATION SHUTOFF in the DISABLED position.
❏
Turn POWER switch of the EPMS housing on.
Now you can adjust the amplifier (see below) and apply test pulses to the cell model. The
upper position the CELL membrane switch (CELL 1) simulates a cell with a resistance of
50 M
Ω
and a capacitance of 22 pF. In the lower position (CELL 2) a cell membrane with
200 M
Ω
and 100 pF is simulated.
