5
5. Slide this sandwich into the Outer Chamber, matching respective colors of the
plug (red and black) to those as indicated on the Outer Chamber. Once
reaching the bottom of the chamber a slight resistance can be felt.
Stabilize the Inner Chamber by inserting the Wedges straight down into the
corner between the Gel Cartridges and the Outer Chamber.
Note: Place the smooth side of each Wegde facing to the Inner Chamber and
the side with the bar facing to the notches of the Outer Chamber.
6. Fill the Inner Chamber with Running Buffer up to 2 mm below the top edge of
the Gel Cassettes. After a few minutes, check for leakage (lowering of the
level of the inside buffer). If leakage is observed, disassemble the inner
Chamber and repeat the above steps.
Note: Avoid a short circuit; do not overfill the Inner Chamber.
7. Fill the Outer Chamber with running buffer up 2.5 cm below the top edge of
the Outer Chamber.
Note: Pour the buffer gently to avoid rapping air bubbles along the bottom of
the Inner Chamber, minor bubbles can be ignored.
8. Load a sample into the well. (Please refer to the Instruction Manual of Precast
Gels for preparation of samples.)
9. Ensure the power cables of the Lid are not connected to the power supply
10. Place the Lid on the Outer Chambers that the plugs are connected with the
jacks firmly.
11. Connect the power cables of the Lid to the power supply with the proper
polarity.
Note: When attaching the Lid, the power supply must be switched
off.
