Nikon TIRF Instructions Manual Download Page 16 | Manualshive

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I Name and Function of Each Part

3 TIRF attachment

- 14 -

Illustration of the specimen cover

(6) Specimen cover

This cover employs a filter that can attenuate the intensity of light in the proximity of 488 nm and
523 nm wavelengths by a factor of up to 1000. Make sure that the cover is always in place,
except when you are adjusting the laser focal point.

(6) Specimen cover

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Summary of Contents for TIRF

Page 1: ...M301 E 03 6 CF 2 TIRF SYSTEM FOR TE2000 INSTRUCTIONS ...

Page 2: ......

Page 3: ...oducts are designed to provide the utmost safety during use incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage To ensure correct use read the instruction manual carefully and thoroughly before using the instrument Do not discard the manual keep it handy for easy reference Safety instructions within this manual are accompanied by the f...

Page 4: ...s a whole conforms to the safety standards for FDA Class II and EN IEC Class 2 laser products Das System ist in Übereinstimmung mit dem Compliance Guide for Laser Products der CDRH Center for Devices and Radiological Health der amerikanischen FDA Food and Drug Administration sowie der europäischen Norm EN60825 1 und der internationalen Norm IEC 60825 1 entworfen und gebaut worden FDA Klasse II Las...

Page 5: ...e stage warn that laser beams are emitted from these areas Never attempt to look into the objective or the areas near the objective from above or below the stage as the lens emits a powerful laser beam toward the top and bottom sides of the stage Your eyes or skin may be exposed to the laser beam resulting in injury Laser warning label Laser product label Aperture label ...

Page 6: ...iert ist Wenn die oben aufgeführten Vorsichtsmaßnahmen nicht eingehalten werden besteht die Gefahr dass Laserlicht aus den Löchern des Objektivrevolvers der Montageöffnung für die Kassettenhalterung oder der Befestigungslöcher des Lampengehäuses austritt 3 Do not remove the TIRF attachment Die TIRF Beleuchtungsvorrichtung Do not remove the TIRF attachment from the microscope Removing the attachmen...

Page 7: ...move the cassette holder when laser is on Während der Bedienung nicht die Kassettenhalterung entfernen Never attempt to remove the cassette holder when the laser is on Removing the cassette holder may lead to exposing your eyes or skin to the laser beam Nicht die Kassettenhalterung entfernen während der Laser eingeschaltet ist Anderenfalls besteht die Gefahr dass Laserlicht aus der Einstecköffnung...

Page 8: ... the power cords specifies below Use of an improper power cord can result in fire or other hazard Using units in areas where the supply voltage is 100 to 120 V UL listed detachable power cord set 3 conductor grounding Type SVT No 18AWG 3 m long maximum rated at 125 V AC minimum Using units in area where the supply voltage is 220 to 240 V Approved according to EU EN standards 3 conductor grounding ...

Page 9: ...n and failure never attempt to use the system under conditions of high temperature and humidity Make sure that the system is not subject to strong vibrations which may degrade image quality Consult with your Nikon representative about preventive measures you may take when your system is installed To prevent degradation malfunction or failure of your laser units always use power supply that is free...

Page 10: ...eflection Interference Observations Using Epi fl Attachment 26 4 1 Objectives 26 4 2 Attachment Setup Procedure 26 III Maintenance 27 1 Replacing the halogen lamp 27 2 Replacing the objective and the filter block 28 3 Moving or relocating the system 28 4 Cleaning the lens section 28 IV Shutter Control Unit Communications Commands 29 1 Communication controlled operation 29 1 1 Communications system...

Page 11: ... adapter 13 Connect to PC serial port 15 Stage up kit 16 Epi fl cassette holder 17 Epi fl attachment 4 Three laser unit 7 Solid state laser model CDPS532M 020 6 He Ne laser G 543 nm Y 594 nm R 633 nm 14 TIRF cassette holder 8 Solid state laser adapter 5 Air cooled argon laser model 161C 163 161C 161C 163 G Y R G Y 18 TV camera 19 Monitor 12 Eyepiece shutter 11 Power cable 1 Inverted microscope TE2...

Page 12: ...lter ND4 ND8 3 Motorized shutter 4 Optical fiber cable 6 ND filter ND2 ND8 ND32 7 ND filter ND16 8 Condenser lens 9 Dichroic mirror Detach the excitation filter 10 Objective 11 Specimen 5 Collimator lens 2 ND filter ND4 ND8 3 Motorized shutter 1 Dichroic mirror for combining excitation light Three laser unit Two laser unit TIRF attachment TIRF cassette holder Laser from either of the units ...

Page 13: ... cable Excitation light emitted by this unit forms an image on the pupil surface of the objective To protect your eyes and skin from exposure to the laser do not detach the TIRF attachment from the microscope TIRF attachment 1 Optical fiber cable 2 Laser position adjustment knobs 3 Field diaphragm centering knob 4 ND filter 5 Condenser lens locking screw 5 Indices 5 Focal point adjustment knob 3 F...

Page 14: ...aser position adjustment knob never attempt to look into the objective or the areas near the objective from above or below the stage as the lens emits a powerful laser beam toward the top and bottom sides of the stage To protect your eyes and skin from exposure to laser beam reflections never place or mount any reflective materials on the stage in the room or on the chamber ceiling 3 Field diaphra...

Page 15: ...a hexagonal screwdriver Move the focal point adjustment knob back and forth while observing the laser beam on the ceiling of the room or chamber Try to minimize the spot illuminated by the laser try to reduce the width of the illuminated spot by at least half When adjustment is complete tighten the condenser lens locking screw with a hexagonal screwdriver Indices circumferential grooves are provid...

Page 16: ...n cover 6 Specimen cover This cover employs a filter that can attenuate the intensity of light in the proximity of 488 nm and 523 nm wavelengths by a factor of up to 1000 Make sure that the cover is always in place except when you are adjusting the laser focal point 6 Specimen cover ...

Page 17: ...blocks 2 Shutter lever Move the lever clockwise to move the shutter into the optical path thus interrupting the laser beam In general leave this lever in the O open position and manipulate the shutter control unit to interrupt the laser beam with the motorized shutter 3 Filter block mount cover Make sure that all lasers are off when replacing filter blocks Remove this cover to replace filter block...

Page 18: ...r 532 nm output manufactured by JDS Uniphase Model CDPS532M 020 Three laser unit This unit can incorporate a total of three lasers namely one air cooled argon laser and two He Ne lasers or one air cooled argon laser one He Ne laser and one solid state laser Air cooled argon laser 488 nm output manufactured by Spectra Physics Model 161C 030 or 163 C02 He Ne laser 633 nm outputs manufactured by JDS ...

Page 19: ...light optical fiber cable This inlet accommodates the optical fiber cable for excitation light 6 Caution label CAUTION Removing the cover to inspect the unit may result in exposure to laser beam and eye injury Never attempt to remove the cover OUT Pull out IN Push in Three laser unit 4 ND filter slider 5 Inlet for excitation light optical fiber cable 6 Caution label 2 He Ne laser 3 He Ne laser Sol...

Page 20: ...s of the AC adapter the eyepiece shutter and the laser unit at the locations shown in the figure To prevent malfunctions always turn off the system power before attaching or detaching any of the cables 1 Power switch Turns the 12 VDC power from the AC adapter on or off 2 Power lamp Lights green when the power is on 3 AC adapter connector Connects the AC adapter output 4 Eyepiece shutter connector ...

Page 21: ...ith the shutter button Disabling shutter switch A command can be issued to disable shutter operation with the shutter switch 8 Shutter switch Opens or closes the motorized shutters With the microscope mounted eyepiece shutter closed so that no light entering the eyepiece you can open or close the selected motorized shutter by repeatedly pressing this switch The eyepiece shutter is a safety device ...

Page 22: ...odule blocks the optical path to the binocular eyepiece during TIRF observation while the mechanism closes the shutter in the laser unit during normal observation observation using eyepieces so that the laser beam is not guided into the TIRF attachment An illumination pillar switch to be mounted on the microscope s illumination pillar is supplied with the eyepiece shutter However ignore this switc...

Page 23: ...ns Items to check before turning on the laser Before turning on the laser check that 1 an objective or cap mounted on each of the sockets on the nosepiece of the microscope 2 that the diascopic illumination lamphouse is attached and 3 that a cassette holder is attached ...

Page 24: ...the controller At this time make sure that Run mode is selected Three lamps on the remote controller will light green in approximately one minute Laser output will stabilize in approximately 15 minutes Argon laser remote controller Be sure to read the Instruction Manual supplied with the air cooled argon laser Always select Run mode when using the argon laser Image quality may be degraded if Run m...

Page 25: ...s He Ne laser power unit Be sure to read the Instruction Manual supplied with the He Ne laser 3 Emitting the Solid State Laser Turn on the switch of the solid state laser s power unit The output will be stable and ready for use in approximately five minutes Solid State ｌaser power unit Make sure to refer to the Instruction Manual accompanying the solid state laser 4 Setting in the ND filter To pre...

Page 26: ...mphouse when the laser is on Never attempt to remove the diascopic illumination lamphouse when the laser is on Removing the lamphouse may cause the laser beam to be directed through the microscope s reflective optical system and emitted through the mounting hole for the lamphouse Do not remove the cassette holder when the laser is on Never attempt to remove the cassette holder when the laser is on...

Page 27: ...r the objective from above or below the stage as the lens emits a powerful laser beam toward the top and bottom sides of the stage To protect your eyes and skin from exposure to laser beam reflections never place or mount any reflective materials on the stage in the room or on the chamber ceiling 8 Adjust the laser position adjustment knobs to provide TIRF illumination See 2 Laser position adjustm...

Page 28: ...be observed with the following optical settings 4 2 Attachment Setup Procedure 1 Fluorescent filter block upper Setup Fit the G excitation filter and dichroic mirror for UV excitation to the filter block Do not put any barrier filters Here remove the TIRF filter block lower from the optical path Explanation With these settings interference fringe components in the light from the specimen image wil...

Page 29: ...s correct Use only the specified lamp The lamp and nearby parts are extremely hot when the lamp is lit or immediately after it has been turned off Before replacing the lamp turn off power and allow these parts to cool Avoid touching the lamp with bare hands Dirt or fingerprints on the lamp will cause uneven illumination and shorten the life of the lamp Wear gloves when handling lamps 1 Switch off ...

Page 30: ... handled by trained service personnel Any attempt by unauthorized staff to perform these tasks during transfer or relocation of the system may result in exposure of the eyes or skin to laser beams or degrade system performance If you need to move or relocate the system please contact your dealer Detach the laser from the laser unit Detach the optical fiber cable from the laser unit Detach the opti...

Page 31: ...r other devices through the RS 232C interface Create your own control program based on the information given in this manual 1 1 Communications system Start stop serial communication 1 2 Connector specification The following signals are used with the system s serial interface connector D sub 9 pin connector male Pin No Signal I O 1 Unused 2 RxD Input 3 TxD Output 4 Unused 5 GND GND 6 Unused 7 Unuse...

Page 32: ...when the system receives data from external equipment is referred to as command format while that used when the system sends data is called response format In the following description indicates a delimiter and not data characters that is actually sent or received 1 5 1 Command format A BBB C C CR A Command identifier one lower case alphabet character ASCII code one byte Identifier Description c O...

Page 33: ...or code ASCII code CR Carriage return 0x0D LF Line feed 0x0A Error codes Code Type Description 1 Command error Command identifier improper command name 2 Parameter error Improper parameter 4 Receive buffer overflow Received data exceeds the permissible amount 32 bytes 6 Command overflow Number of commands that can be processed is exceeded during continuous transmission of commands five commands 7 ...

Page 34: ...ime Unit 0 1 sec 100 0 sec max 6 Error code cTSW 4 oTSW nTSW 6 Enables disables the laser open close switch on the control panel 4 1 Enable 0 Disable 6 Error code 2 r command Data request command r command a Normal response n Error response Description of operation rTSR aTSR 1 nTSR 6 Calling of whether any shutter is open 1 0 All shutters closed 1 3 Number of open shutter 6 Error code rTTS 1 aTTS ...

Page 35: ...t Switch the optical path to the TV camera port Inappropriate filters dichroic mirror and absorption filter and laser wavelength setting Use filters and wavelength appropriate for the fluorescence reagent used Excessively low laser power Adjust the ND filters of the laser unit and the TIRF attachment Epi fl attachment s filter block in optical path Remove the filter block from the optical path Dar...

Page 36: ...anufacturer Spectra Physics Two laser unit Model Laser head 161C 030 laser power source 263 C04 Remote controller 361C Three laser unit Model Laser head 161C 030 laser power source 263 C04 Remote controller 361C Laser head 163 C02 laser power source 263 C04 Remote controller 361C He Ne laser system Manufacturer JDS Uniphase Model Operation wavelength 594 nm Laser head 1677P Laser power source 1207...

Page 37: ...rective EN61010 1 EN60825 1 1994 A2 GS Approved EMC Electro Magnetic Compatibility EN61326 EN55011 Group 1 Class B EN61000 4 2 4 3 4 4 4 5 4 6 4 8 4 11 Australia EMI regulation AS NZS2064 Class B FCC Part 15B Class A Canada EMI regulation This equipment has been tested and found to comply with the limits for a Class A digital devices pursuant to Part 15 of the FCC Rules These limits are designed t...

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