Chapter 5 Troubleshooting
100
Problem
Cause
Countermeasure
The lamp voltage is too low.
Adjust the brightness with the brightness
control dial.
(p.30)
An ND filter is placed in the optical path.
Remove the ND filter from the optical path.
(p.40)
The brightness is insufficient.
(Refer to the troubleshooting for
the electric system too.)
The aperture diaphragm is stopped down too
far.
Open the diaphragm to a suitable size.
(p.42)
A polarizer, analyzer or the fluorescent unit is
placed in the optical path although the
bright-field microscopy is to be performed.
Remove the polarizer, the analyzer, or the
fluorescent unit from the optical path.
(p.43,
50)
A halogen lamp is used for a dark sample.
Replace the light source to the brighter one.
(p.56)
The used objective is not suitable for the
microscopy.
Use the designated objective.
(p.32)
The room is too bright. (for the dark-field
microscopy or the epi-fl microscopy)
Darken the room.
Diascopic microscopy
The condenser position is too low.
Adjust the condenser focus knob so that the
field diaphragm image is focused on the
sample surface.
(p.59)
The eyepiece diopters are not adjusted.
Adjust the diopters.
(p.35)
The objective hits the sample
when switched from low to high
magnification. The sample goes
out of focus when switching
objectives.
The eyepieces are not attached correctly.
Mount the eyepieces correctly by aligning the
positioning grooves.
(p.81)
The sample does not move
smoothly.
The sample holder is not secured correctly on
the stage.
Secure the sample holder correctly.
(p.80)
The interpupillary distance is not adjusted.
Adjust the interpupillary distance.
(p.34)
When viewing through the
binocular eyepiece, the image
does not resolve into a single
image.
The eyepiece diopters are not adjusted.
Adjust the diopters.
(p.35)
The interpupillary distance is not adjusted.
Adjust the interpupillary distance.
(p.34)
The eyepiece diopters are not adjusted.
Adjust the diopters.
(p.35)
The brightness is not appropriate.
Adjust the brightness with the brightness
control dial or ND filters.
(p.30, 40)
Eye strain develops while viewing.
Eyepieces with different view-field numbers
are used for the left and right eyes.
Use eyepieces with the same view-field
number.
The coarse focus ring is heavy in
rotation.
The coarse torque adjustment ring is tightened
too much.
Loosen the coarse torque adjustment ring
adequately. (p.36)
The revolving nosepiece falls on
its own weight and the image goes
out of focus.
The coarse torque adjustment ring is loosened
too much.
Tighten the coarse torque adjustment ring
adequately. (p.36)
No polarizer or analyzer is placed in the
optical path.
Place it into the optical path.
(p.43)
The analyzer and the polarizer are not at the
crossed Nicol’s position.
Adjust the orientation of the polarizer to make
the crossed Nicol’s position.
(p.44)
No DIC prism is placed in the optical path.
Place it into the optical path.
(p.46, 47)
The 1/4
λ
plate is not attached to the polarizer.
(For Senarmont method)
Place the polarizer equipped with a 1/4
λ
plate
into the optical path.
(p.43)
No interference color is seen in
the DIC microscopy.
The combination of the objective and the DIC
prism is wrong.
Place an appropriate objective into the optical
path. (p.46)
