45
III. Operation of Each Part
23
Image Capturing
Images under the microscope observation can be captured by attaching a camera head to the
trinocular eyepiece tube.
For detailed information, refer to the operating manual provided with the camera head or camera
control software.
Proper adjustment of light intensity and focus on the microscope are important for obtaining clear
images. Listed below are key considerations in capturing clear images.
Adjusting light intensity
•
Lamp voltage:
If accurate color reproduction is important, adjust the brightness control knob
to the “three o’clock” position. When adjusting the brightness, use ND filters.
•
Filter:
Place a commercially available color compensation filter on the filter pocket at
the microscope base, as necessary.
Adjusting the condenser
•
Focus and center the condenser always.
•
For normal operations, set the diaphragm aperture to 70 to 80% of the N.A. of the objective.
Confirming the photomicrographic range
The image on the monitor represents the photomicrographic range.
Confirming the focus
Check the focus by viewing through the eyepiece and viewing the monitor. If the focal positions for
the two images differ, adjust the focal position adjustment screw at the camera port.
Making Adjustments to Keep out Ambient Light
•
Field diaphragm:
Stop down the diaphragm to a setting just slightly wider than the area shown
on the monitor.
•
Eyepiece:
Cover the eyepiece with a piece of cloth.
Anti-vibration measures
If the exposure is less than 1/8 of one second, reduce light intensity with ND filters to make
exposures longer than 1/8 of one second. (If accurate color reproduction is not important, you can
use the brightness control knob to reduce light intensity.)
