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14. As needed, engage the Z-stepper-motor (used to do Z-stacks) by pulling up gently
(on back right of microscope).
Shutdown:
1. If no one is signed up 2 hours after your session is over:
a. Raise objectives fully up and move up the focus.
b. Clean off oil objectives with lens paper and replace lens in its holder.
c. Reverse of Turn on, items 6
1.
2. If someone is signed up after you:
a. Turn off EZ-C1 software.
b. Leave everything else on.
Overview
The C1si is a confocal laser-scanning microscope with four lasers for multi-color
imaging. It has 4 lasers with these laser lines: 408nm, 457nm, 488nm, 514nm, 561nm,
638nm. It has 2 types of detectors: one with 3 standard band pass detectors and one
with a spectral detector that can image 32 different channels at the same time (they can
be set to be 2.5nm, 5nm, and 10nm wide). It is on an upright Nikon FN1 microscope with
a large electrophysiology stage. The max penetration depth into tissue is approximately
100–200 µm depending on the opacity of the sample.
To move the objective turret between the two lens positions, center the lever so that both
lenses are in the up position and then press the button on the upper right part of the
carriage and move forward or back. Make sure that it is in the very center so that when
you change positions it does not fall down and get damaged.
Note: the viewed area may change because of slight differences in lens position
between the two holders.
