AccuPoint 2 User’s Guide
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INTRODUCTION
Neogen’s AccuPoint 2 ATP Sanitation Monitoring System is a lightweight, handheld
system for validating a sanitation program’s effectiveness by detecting food residue
and microorganisms on surfaces and in liquids. The system’s easy-to-use AccuPoint
samplers automatically mix self-contained reagents to quickly generate results.
When used with the AccuPoint Data Manager V2 software, the AccuPoint instrument can
record the testing results at specific site locations, along with the date, time and user.
The test site information is entered in the Data Manager program and uploaded to the
AccuPoint instrument. The user simply toggles to the correct site and takes the test.
Up to 999 unique testing sites can be tracked with each AccuPoint instrument. Results
from up to 4,000 tests can be retained at any time and are not lost if the instrument
loses power. Four rechargeable nickel metal hydride (NiMH) batteries provide power.
Operating principles and technology
The AccuPoint 2 system utilizes ATP bioluminescence to determine the cleanliness
of test samples. Adenosine triphosphate (ATP) is a chemical compound found in all
living cells, including bacteria, food debris, yeast and mold. Bioluminescence is a
chemical reaction that produces light. ATP bioluminescence occurs when ATP from a
sample comes into contact with luciferase, an enzyme found in fireflies, and luciferin,
a substrate. The amount of light emitted in this reaction is proportional to the amount
of ATP detected in a sample. After a sample is taken the sampler is pressed into its
cartridge, breaking its seal and initiating the mixing of reagents. The cartridge contains
a buffering solution, which flushes the ATP onto a pad containing the reagents. The
reaction takes place within the cartridge and a detector measures the amount of light
produced. The reading is displayed on the LCD display in relative light units (RLU).
According to preset limits, an icon is displayed indicating a pass, marginal or fail result.
These limits are defined by the operator or by using the system presets.
Sampler
Compartment
Eject Button
Toggle Button
Battery
Compartment
Full-color
Display
Power Button
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