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NanoEnTek ADAMII, User Manual

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NanoEnTek ADAMII User Manual Download Page 14

14

Calibration

Calibration    
 procedure

6. Attach dry-proof closure tape to prevent drying.

[Attach dry-proof closure tape]

Low volume

Correct volume

Warning: 

• Calibration bead loading error

   - Ensure that 25μL of calibration bead is fully loaded on the ADAMII™ 
     assay slide.   
   - The instrument will not detect in low or high loading volumes.  

CAUTION: 

• Avoid bubbles as they may negatively affect results.

• Boundary between black and white on the tape should be lined up 

   with the edge of the slide. 

Dry-proof closure tape 

Summary of Contents for ADAMII

Page 1: ...ADAMII 2016 NanoEnTek Inc 1 User manual Bench top Fluorescence Cell Counter...

Page 2: ...e that all the information contained in the ADAMII User Manual is correct at the time of printing However NanoEntek Inc reserves the right to make any changes necessary without notice as part of ongoi...

Page 3: ...peration Insert assay slide Measure menu Initial mode Focusing mode Data menu Setting menu Cleaning Maintenance Troubleshooting Error code Warranty Safety precautions Safety symbols Product specificat...

Page 4: ...a maxi mum of 136 images and which increases the accuracy of analysis The obtained images are processed by image analysis software integrated in the system All proce dures are automatic once the stain...

Page 5: ...low were shipped If any component is missing or damaged contact your local distributor or e mail ivdst nanoentek com Product components ADAMII 1 EA Operating laptop Please note Laptop will be packaged...

Page 6: ...and connection of camera and serial perform cor rectly Perform all procedures of automatic counting Insert and eject the slide from the ADAMII Functions as load and unload State LED Insert Eject butt...

Page 7: ...Serial Camera Control the ADAMII operating Control the camera of the ADAMII Power on off Power supply Serial connection port Camera connection port Power supply port Power switch Power supply port Po...

Page 8: ...material foam in the box 2 Carefully lift the instrument out of the box 3 Place the instrument on a flat level surface 1 Plug the power cord into the electrical outlet and connect two USB cables 2 Co...

Page 9: ...be ejected automatically 2 Double click ADAMII icon on Desktop to start the software CAUTION You must turn on the ADAMII before running the software program Note The ADAMII will be ready to use after...

Page 10: ...to log in Default ID and password are both master Click User management when you need to manage accounts e g ID addition password change To create a new ID fill out the blank user name user ID passwo...

Page 11: ...ick Audit trail to search and trace detailed information of each user 1 Enter the User ID and set the date that you want to search 2 Click Search button 3 Information of the selected user will appear...

Page 12: ...12 Calibration 1 For calibration go to Setting menu and click Calibrate 2 Then calibration window will appear Calibration procedure...

Page 13: ...ly or vortex briefly for 5 seconds before use 4 Once again vortex for 4 seconds and pipette for 25 L immediately 5 Load 25 L of calibration beads on the ADAMII assay slide Shake bottle vigorously Vort...

Page 14: ...ng Calibration bead loading error Ensure that 25 L of calibration bead is fully loaded on the ADAMII assay slide The instrument will not detect in low or high loading volumes CAUTION Avoid bubbles as...

Page 15: ...for 20 seconds need time for beads to settle 8 Insert the slide with calibration beads into the slide port of the instrument and press Insert sample button 9 Enter lot number of calibration beads 10...

Page 16: ...ircumference of the beads Dark and clear contrast to the background Bright Bad Focus Blurry and unclear bead circumference FL Good Focus Bright fluorescence beads with clear contrast to the background...

Page 17: ...after adjusting focus The focus of other fluorescence channels will be set automatically 14 Press the Run button to start the auto calibration Calibration will take about 3 minutes to count the 24 fra...

Page 18: ...15 When the calibration is completed the results will be displayed 16 When you need previous results of calibration click Record button Note If the results are not within the range on the tube please...

Page 19: ...nc 19 Calibration Calibration procedure 17 Calibration result list records will appear 18 Click Save button to save the calibration records The result will be saved in CSV format 19 Press Close button...

Page 20: ...ample into the ADAMII assay slide and attach a dry proof closure tape Load 25 L sample Attach dry proof closure tape Note Please refer to page 14 to check cautions for loading correct volume and attac...

Page 21: ...he assay slide into the slide holder Note Assay slide should be inserted to the end of the slide holder in the direction of arrow on the slide facing up Incomplete insertion of slide may cause erroneo...

Page 22: ...lustrations of various possible cases of erroneous assay slide insertion are presented below Warning Using ADAMII assay slide Do not reuse the ADAMII assay slide as leftover dye from the previous read...

Page 23: ...on on the screen The slide will automatically be moved into the instrument Note LED light of the Insert Eject button shows the status of the slide holder Slide inserted Off Slide ejected On Slide inse...

Page 24: ...asure menu 1 Counting mode 2 Display area 3 Detection channel 4 Insert sample 5 Result area 6 Test information Operation Measure menu Initial mode 1 4 5 6 2 3 Note Above screen and upcoming screen ima...

Page 25: ...ber of frames Viability Check the circle to include to get viability data Captured image of sample will be displayed It will be activated after inserting sample Refer to page 28 The selected channel w...

Page 26: ...the sample will be displayed It will be activated after inserting sample Refer to page 33 Date sample patient ID and operator information will be displayed Operation Measure menu initial mode Result...

Page 27: ...is inserted focusing mode will appear 1 Display area 2 Detection channel 3 Focus option 4 Save focus 5 Run sample 6 Eject Operation Measure menu Focusing mode 1 4 5 6 2 3 Note Above screen and upcomi...

Page 28: ...nnels correspond to the markers below PerCP CD45 PE CD34 FITC live dead marker 1 Focus can be adjusted manually or automatically Focusing knob is right side of the instrument Operation Measure menu Fo...

Page 29: ...is selected 2ch manual focus Set the focuses for two channels Bright and PerCP manually then the other two fluorescent channels will be automatically focused when this option circle is selected Opera...

Page 30: ...ad focus 1 Unclear cell image no contrast to the background Bad focus 2 Brightandwhitecellbodyundistinguishabletothebackground Bad focus 3 Blurry and unclear cell image Bright Good Focus Bright Bad Fo...

Page 31: ...od Focus Clear cell image bright cell body in contrast to the dark background Bad focus Unclear cell image blurry cell image and donut shaped cell image CD45 PerCP Good Focus CD34 PE Good Focus FITC G...

Page 32: ...ase check the circle under the viability circle 2 Press the Run sample button 3 Enter the sample ID patient ID antibody lot number and slide lot number 4 Counting the 136 frames is recommended When th...

Page 33: ...e finished assay slide insert the new sample loaded assay slide repeat focusing and running procedure Click Eject button when you want to manually eject the sample There is no need to use this button...

Page 34: ...s are able to review save and export the data 1 Test information 2 Sample information 3 Captured image 4 Sample volume information 5 Result area 6 Histogram 7 Raw data table 8 Data list 9 Export data...

Page 35: ...plays sample ID Patient ID displays patient ID Operator displays operator s ID User can check the frame numbers and sample volumes of captured images Sample type display the sample type HPC A Mobilize...

Page 36: ...y the cells counted through a particular channel ADAMII circles the cells that were analyzed as follows Yellow cells counted in bright channel Purple cells counted in PerCP channel CD45 inADAMII CD34...

Page 37: ...enu Captured image Double click the captured image then you can check preview images of selected channel on the right side of the screen To check the preview im ages of other channels select that chan...

Page 38: ...HPC A total volume 275 L sample volume 20 L Mobilized PB total volume 60 L sample volume 20 L Control total volume 275 L sample volume 20 L Result area displays reportable counting results of the sel...

Page 39: ...n and select Delete menu Then the Result area will show the re calculated data reflecting the deleted frame s User can check the cell size graph of each channel Cell size gating is available by managi...

Page 40: ...ion in specific area of sample that frame should be deleted 2 Dust when there is dust inside the slide that frame should be deleted 3 Bubble After loading the sample if there is bubble in the slide th...

Page 41: ...peration 4 Cell debris when there is cell debris that frame should be deleted 5 Overlapped circle when there are several circles in one cell that frame should be deleted Data menu Raw data table Br im...

Page 42: ...f Click All button to export the data in 3 types of formats at once csv file data table containing comprehensive information in a spreadsheet jpg file raw images of each channel collected for each fie...

Page 43: ...name a file select the data file and click Rename button to change name of the selected data Select all Click Select all button to select all of the listed data You can also select all of the sample d...

Page 44: ...l user preferences and other Settings It allows users to edit settings about the basic operation software and firmware information 1 Date Time 2 Counting settings 3 Calibration 4 Sample volume 5 Entry...

Page 45: ...es instrument more sensitive to objects While adjusting the sensitivity lower makes the instrument less sensitive and is useful if there is a lot of background Circularity used to determine which obje...

Page 46: ...the data of calibration briefly including date time lot number user ID and result of each channel Select the sample type Enter the total volume sample volume RBC lysis buffer and sample volume Then cl...

Page 47: ...ive and plug into the laptop 2 Press SW update button 3 Find the updated software in the USB drive and double click the file to execute 4 When you see the window below the update completed successfull...

Page 48: ...to the laptop 2 Press FW update button 3 Move to the folder where Firmware update file is located and click the file 4 Press OK button when the automatic update is completed 5 Program will be restart...

Page 49: ...connecting status between cable and the instrument Please check the connec tion status if Disconnected is displayed Disk info indicates available hard disk space Please back up or delete your data fr...

Page 50: ...le or service ADAMII by yourself Unauthorized repairs may damage ADAMII or alter its functionality which will void your warranty Contact ivdst nanoentek com or your local distributor to arrange for se...

Page 51: ...still having problem request technical support Set the focus with 2ch manual focus function Request technical support First check the sample type Second if you used the bottom of ADAMII CD34 Reagent t...

Page 52: ...econd steps change the bead If none of the above 3 steps solve the problem please request technical support ADAMII does not insert and count the slide when the slide holder cap opened But if sensor of...

Page 53: ...era connection fail E0002 Serial connection fail E0010 Serial disconnected E0020 Camera disconnected E0030 Open the slide holder E0031 Open the slide door Error code Reason Message Position Driver Sta...

Page 54: ...54 Error code E0040 Data storage is full E0050 Sample density is too high E0060 Update fail Error code Reason Message Position Data save Measuring SW update...

Page 55: ...arts 4 Use of fittings or spare parts supplied by anyone other than NanoEnTek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For you...

Page 56: ...instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet a...

Page 57: ...agement de l instrument N inserez jamais aucun objet dans les orifices d aeration de l instrument car cela pourrait entrainer un choc electrique des blessures chez les utilisateurs et des dommages d e...

Page 58: ...led bin symbol is attached to a product it means the product is covered by the European Directive 2012 19 EU 2 All electrical and electronic products should be disposed of separately from the municipa...

Page 59: ...onochrome CCD Stage Automated X Y Z stage Objective lens Olympus lens 10X 35 tube lens Exported formats Image JPEG BMP Raw data CSV Operating power 100 240 V 1 5 A 50 60 Hz Electronic input 12VDC 5 0A...

Page 60: ...r Main instrument Power cord USB connection cable CPU 6th Generation Intel Core i3 6100U Processor OS Windows 8 1 64 bit RAM 4 GB DDR3L 1600MHz HDD 500GB 7200rpm Hard Drive 14 Full HD 1920 X 1080 Anti...

Page 61: ...or technical assistance please call or email Email ivdst nanoentek com Website www nanoentek com Manufactured by NanoEnTek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82...

Page 62: ...62 Memo...

Page 63: ...ADAMII 2016 NanoEnTek Inc 63 Memo...

Page 64: ...aseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7942 Fax 82 2 6220 7999 MT Promedt Consulting GmbH Altenhofstrasse 80 66386 St Ingbert Germany US Branch NanoEnTek USA Inc 5627 Stoneridge Drive Suite 3...

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