12
view or lighting is not
even, can not see full
field of view.
not clean
Nosepiece position is not right
Lift nosepiece to the highest
position
Attachment has not been set properly
Push the attachment stick the slot
surface
Slider loosen
Tighten slider draw in clockwise
Oil or dust is found in
the field of view.
There is oil or dust on the eyepiece
lens.
Wipe the eyepiece.
Defocusing or low
resolution
The objective is damaged.
Repair the objective (by a
professional).
There is oil or dust on the surface of
the lens of the
Wipe the objective or the
eyepiece.
The aperture of the aperture
diaphragm is too small.
Adjust the aperture of the aperture
diaphragm based on the objective
magnification (or numerical
aperture) used.
The objective deviates from the light
path.
Turn the nosepiece to the fixed
position.
Sample slider cover glass is too thick
or thickness
Prepare sample slider according
to objective parameters
The focal plane of the
image is inclined
(brighter on one side and
darker on the other)
。
The lighting bulb is seriously inclined. Reposition the lighting bulb.
The specimen is not laid flatly.
Lay the specimen flatly on the
object stage and hold it stably.
Surrounding is too bright
Create darker surrounding when
observing fluorescence
Phase contrast
performance is not good
enough
Phase contrast condenser is not
matched with phase contrast ring
Choose matched times phase
contrast condenser and phase
contrast ring
Phase contrast plate central is out of
phase contrast condenser light center
Adjust phase contrast center to
make two light circle overlay to
each other.
Sample is not suitable for phase
contrast observation
Change suitable sample
Mechanical
system
The image cannot
remain clear during
observation.
The focusing mechanism flows (slides
down) automatically.
Adjust the coarse adjusting hand
wheel.
The fine focusing mechanism fails
Check and have professional
Summary of Contents for MF52-N
Page 2: ...Inverted Fluorescence Microscope MF52 N ...
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